{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:51:25Z","timestamp":1791546685742,"version":"4.3.4"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072483"],"award-info":[{"award-number":["32072483"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1400600"],"award-info":[{"award-number":["2023YFD1400600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Arthropods utilize olfactory function to detect informational substances within their natural habitats, thereby facilitating essential life activities such as foraging, mating, and oviposition. Niemann\u2013Pick protein type C2 (NPC2), a putative odorant carrier, has not been fully described in the olfactory perception of predatory mites. In this study, we characterized in detail the olfactory perception functions of NbNPC2\u20102 and NbNPC2\u20103 in the important predatory mite\n                    <jats:italic>Neoseiulus barkeri<\/jats:italic>\n                    . The results show that 2 NPC2 proteins, with 6 conserved cysteine sites, are significantly upregulated (by 2.93\u2010fold for\n                    <jats:italic>NbNPC2\u20102<\/jats:italic>\n                    and 1.66\u2010fold for\n                    <jats:italic>NbNPC2\u20103<\/jats:italic>\n                    ) with methyl salicylate (MeSA), an important herbivore\u2010induced plant volatile (HIPV). Knocking down both\n                    <jats:italic>NbNPC2\u20102<\/jats:italic>\n                    and\n                    <jats:italic>NbNPC2\u20103<\/jats:italic>\n                    prevented\n                    <jats:italic>N. barkeri<\/jats:italic>\n                    from preferring pest\u2010infested plants, and reduced their crawling speed to 2.92 and 3.12 mm\/s under MeSA treatment. The affinity analysis revealed that NPC2\u20102 binds to HIPVs\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010ionone, MeSA, and ocimene (Ki = 28.55 \u00b1 2.12, 40.73 \u00b1 3.89, and 45.15 \u00b1 2.22, respectively), and NPC2\u20103 binds to 4\u2010ethylbenzaldehyde (Ki = 51.67 \u00b1 0.23). Molecular docking further confirmed hydrogen bonding interactions between NPC2\u20102, NPC2\u20103, and these HIPVs. These results indicated that NPC2\u20102 and NPC2\u20103 act as odor carriers involved in the olfactory perception of mites toward HIPVs. This enhances understanding of the molecular mechanism of phytoseiid olfaction.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70143","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T11:28:47Z","timestamp":1754393327000},"page":"1915-1926","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Functional characterization of Niemann\u2013Pick proteins type C2 as odorant carriers in\n                    <i>Neoseiulus barkeri<\/i>\n                    olfactory perception"],"prefix":"10.1111","volume":"33","author":[{"given":"Haozhuo","family":"Yang","sequence":"first","affiliation":[{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, College of Plant Protection Southwest University  Chongqing China"},{"name":"Yibin Academy of Southwest University  Yibin Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiandi","family":"Niu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, College of Plant Protection Southwest University  Chongqing China"},{"name":"Yibin Academy of Southwest University  Yibin Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongjiang","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, College of Plant Protection Southwest University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Xiang","sequence":"additional","affiliation":[{"name":"Institute of Vegetable Xizang Autonomous Region Academy of Agriculture and Animal Husbandry Sciences  Lhasa China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7031-4556","authenticated-orcid":false,"given":"Huai","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, College of Plant Protection Southwest University  Chongqing China"},{"name":"Yibin Academy of Southwest University  Yibin Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanqiu","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Vegetable Xizang Autonomous Region Academy of Agriculture and Animal Husbandry Sciences  Lhasa China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6594-4729","authenticated-orcid":false,"given":"Yaying","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, College of Plant Protection Southwest University  Chongqing China"},{"name":"Yibin Academy of Southwest University  Yibin Sichuan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10886-016-0669-z"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2022.103801"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12677"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JOEC.0000017976.60630.8c"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JOEC.0000048784.79031.5e"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00979614"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-024-03871-2"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2017.07.010"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-010715-023523"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evw048"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2016.09.004"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1323928111"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2014.05.013"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.08.011"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-120811-153635"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.11158\/saa.25.8.5"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2023.105451"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6352"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1086\/324068"},{"key":"e_1_2_7_21_1","first-page":"556","article-title":"A spatzle protein involved in the immune response of Neoseiulus barkeri (Acari: Phytoseiidae) against Beauveria bassiana","volume":"28","author":"Niu T.D.","year":"2023","journal-title":"Systematic and Applied Acarology"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants10040754"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00320"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12339"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1570-7458.1998.00360.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12368"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-020117-043322"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00172"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5325"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0180654"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6104"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20200170"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4100"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1017\/S000748531800072X"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13156"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbd.2019.01.004"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033589"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2018.03.004"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10526-021-10117-3"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-019-6281-1"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12473"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00052"}],"container-title":["Insect Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.70143","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1744-7917.70143","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.70143","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:00:25Z","timestamp":1791543625000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-7917.70143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,5]]},"references-count":41,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/1744-7917.70143"],"URL":"https:\/\/doi.org\/10.1111\/1744-7917.70143","archive":["Portico"],"relation":{},"ISSN":["1672-9609","1744-7917"],"issn-type":[{"value":"1672-9609","type":"print"},{"value":"1744-7917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,5]]},"assertion":[{"value":"2025-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}