{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T09:57:24Z","timestamp":1776419844191,"version":"3.51.2"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The sterile insect technique (SIT) is a highly effective biologically\u2010based method for the suppression of many insect pest populations. SIT efficacy could be improved by methods of male sterilization that avoid the use of irradiation that can result in diminished fitness and mating competitiveness. Alternative sterilization methods include conditional disruption of genes for male fertility, or using their sperm\u2010specific promoters to drive the expression of genes for lethal effectors. Testing has begun for the testis\u2010specific\n                    <jats:italic>\u03b22\u2010tubulin<\/jats:italic>\n                    gene, though additional male fertility genes are required for redundancy or replacement, and for species where the\n                    <jats:italic>\u03b22\u2010tubulin<\/jats:italic>\n                    isoform does not exist or is not testis\u2010specific. Here we had the goal of identifying and characterizing the sequence and transcriptional expression of two genes in the caribfly,\n                    <jats:italic>Anastrepha suspensa<\/jats:italic>\n                    , that are cognates of\n                    <jats:italic>D. melanogaster<\/jats:italic>\n                    spermatocyte\u2010specific male fertility genes. In\n                    <jats:italic>Drosophila<\/jats:italic>\n                    ,\n                    <jats:italic>wampa<\/jats:italic>\n                    encodes a coiled\u2010coil dynein subunit required for axonemal assembly essential to microtubule\u2010based sperm motility, while\n                    <jats:italic>Pros\u03b16T<\/jats:italic>\n                    is a proteasome subunit gene required for spermatid individualization and nuclear maturation. In\n                    <jats:italic>A. suspensa<\/jats:italic>\n                    a cognate to\n                    <jats:italic>wampa<\/jats:italic>\n                    exhibited testis\u2010specific transcript expression, which was minimal in both male and female body tissue. A\n                    <jats:italic>Pros\u03b16T<\/jats:italic>\n                    cognate was not apparent in\n                    <jats:italic>A. suspensa<\/jats:italic>\n                    , but its constitutive isoform,\n                    <jats:italic>Pros\u03b1<\/jats:italic>\n                    6, expresses in male testes, but also in male and female body tissue. Thus, for\n                    <jats:italic>A. suspensa<\/jats:italic>\n                    ,\n                    <jats:italic>wampa<\/jats:italic>\n                    remains a strong candidate gene for male sterility strategies for SIT including a direct target for gene\u2010editing knockout and use of its promoter for testis\u2010specific toxicity or cell death in conditional expression systems.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70161","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T07:12:38Z","timestamp":1757315558000},"page":"505-516","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sequence and expression analysis of potential spermatogenesis\u2010specific gene cognates in the Caribbean fruit fly,\n                    <i>Anastrepha suspensa<\/i>"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2644-6038","authenticated-orcid":false,"given":"Alfred M.","family":"Handler","sequence":"first","affiliation":[{"name":"USDA\/ARS, Center for Medical Agricultural and Veterinary Entomology  Gainesville Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard B.","family":"Furlong","sequence":"additional","affiliation":[{"name":"USDA\/ARS, Center for Medical Agricultural and Veterinary Entomology  Gainesville Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Entomology &amp; Nematology University of Florida  Gainesville Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel A.","family":"Hahn","sequence":"additional","affiliation":[{"name":"Department of Entomology &amp; Nematology University of Florida  Gainesville Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-78225-6_8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781003035572-11"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2020.04.006"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.2009.23"},{"key":"e_1_2_8_6_1","first-page":"107","article-title":"Proteasome gene duplications in mammals, flies and plants","volume":"1","author":"Belote J.M.","year":"2005","journal-title":"Recent Research Developments in Genes & Genomes"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.01-08-0423"},{"key":"e_1_2_8_8_1","first-page":"149","article-title":"A review of the invasion of Drosophila suzukii in Europe and a draft research agenda for integrated pest 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