{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T14:56:14Z","timestamp":1767884174461,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003579","name":"University of Toronto","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003579","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Calcitonin\u2010like diuretic hormone (CT\/DH) is a pleiotropic neuropeptide in insects that plays a role in regulating diuresis in\n                    <jats:italic>Rhodnius prolixus<\/jats:italic>\n                    , a blood\u2010feeding insect and a vector of Chagas\u2019 disease. Here, we examine the role of this neuropeptide in another feeding\u2010dependent event, that of reproduction in female\n                    <jats:italic>R. prolixus<\/jats:italic>\n                    . CT\/DH\u2010like immunoreactivity is present in the female reproductive system, including cells of the tropharium and pedicel of the ovariole, and nerve processes over the oviducts and calyx; tissues involved in egg maturation, ovulation, and egg laying. qPCR analysis reveals that the transcripts for the Rhopr\u2010CT\/DH receptors,\n                    <jats:italic>Rhopr\u2010CT\/DH\u2010R\u20101B\/C<\/jats:italic>\n                    and\n                    <jats:italic>Rhopr\u2010CT\/DH\u2010R\u20102B<\/jats:italic>\n                    are present in reproductive tissues and fat body, and appear to be regulated post blood meal, a stimulus that triggers diuresis and reproduction. Knockdown of the two receptors using RNA interference reduces the number of eggs produced and their hatching rate. Downregulation of\n                    <jats:italic>Rhopr\u2010CT\/DH\u2010Rs<\/jats:italic>\n                    does not alter protein levels, including vitellogenin, in the fat body or hemolymph and does not change transcript expression of\n                    <jats:italic>RhoprVg1<\/jats:italic>\n                    in the fat body. Knockdown does lead to a decrease in transcript expression of\n                    <jats:italic>RhoprVg1<\/jats:italic>\n                    and the Vg receptor,\n                    <jats:italic>RhoprVgR<\/jats:italic>\n                    , in the ovaries.\n                    <jats:italic>In vitro<\/jats:italic>\n                    analyses show that incubation of tissues with Rhopr\u2010CT\/DH leads to a notable but not significant increase in transcript expression of\n                    <jats:italic>RhoprVg1<\/jats:italic>\n                    and a significant increase in\n                    <jats:italic>RhoprVgR<\/jats:italic>\n                    in the ovaries but has no effect on fat body\n                    <jats:italic>RhoprVg1<\/jats:italic>\n                    or vitellogenin release. Rhopr\u2010CT\/DH causes a dose\u2010dependent increase in phasic contraction of the oviducts and increases the amplitude and frequency of intrinsic rhythmic contractions. Collectively, these results suggest the involvement of Rhopr\u2010CT\/DH in coordinating aspects of reproduction in female\n                    <jats:italic>R. prolixus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70211","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T11:35:30Z","timestamp":1767872130000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Calcitonin\u2010like diuretic hormone influences reproduction in adult female\n                    <i>Rhodnius prolixus<\/i>"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8510-2721","authenticated-orcid":false,"given":"Areej N.","family":"Al\u2010Dailami","sequence":"first","affiliation":[{"name":"Department of Biology University of Toronto Mississauga  Mississauga Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Orchard","sequence":"additional","affiliation":[{"name":"Department of Biology University of Toronto Mississauga  Mississauga Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela B.","family":"Lange","sequence":"additional","affiliation":[{"name":"Department of Biology University of Toronto Mississauga  Mississauga Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.jinsphys.2007.10.012","article-title":"Vitellogenesis in the hematophagous Dipetalogaster maxima (Hemiptera: Reduviidae), a vector of Chagas\u2019 disease","volume":"54","author":"Aguirre S.A.","year":"2008","journal-title":"Journal Insect Physiology"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","DOI":"10.3389\/finsc.2022.1096089","article-title":"Investigating the role of glycoprotein hormone GPA2\/GPB5 signaling in reproduction in adult female Rhodnius prolixus","volume":"2","author":"Al\u2010Dailami A.N.","year":"2022","journal-title":"Frontiers in Insect Science"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2023.1279929","article-title":"Corticotropin\u2010releasing factor\u2010like diuretic hormone acts as a gonad\u2010inhibiting hormone in adult female Rhodnius prolixus","volume":"14","author":"Al\u2010Dailami A.N.","year":"2023","journal-title":"Frontiers in Endocrinology"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.ygcen.2024.114611","article-title":"RhoprCAPA\u20102 acts as a gonadotropin regulating reproduction in adult female, Rhodnius prolixus","volume":"358","author":"Al\u2010Dailami A.N.","year":"2024","journal-title":"General and Comparative Endocrinology"},{"key":"e_1_2_8_6_1","first-page":"21","article-title":"The role of ecdysteroid in the regulation of ovarian growth and oocyte maturation in Rhodnius prolixus, a vector of Chagas disease","volume":"225","author":"Benrabaa S.A.M.","year":"2022","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1038\/s41598-019-38761-x","article-title":"Multiple neuropeptides produced by sex\u2010specific neurons control activity of the male accessory glands and gonoducts in the silkworm Bombyx mori","volume":"9","author":"\u010ci\u017em\u00e1r D.","year":"2019","journal-title":"Scientific Reports"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1242\/jeb.204.10.1795","article-title":"The Drosophila melanogaster homologue of an insect calcitonin\u2010like diuretic peptide stimulates V\u2010ATPase activity in fruit fly Malpighian tubules","volume":"204","author":"Coast G.M.","year":"2001","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.jinsphys.2006.10.002","article-title":"The interaction of feeding and mating in the hormonal control of egg production in Rhodnius prolixus","volume":"53","author":"Davey K.","year":"2007","journal-title":"Journal of Insect Physiology"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","DOI":"10.1242\/jeb.151092","article-title":"Evolution of neuropeptide signalling systems","volume":"221","author":"Elphick M.R.","year":"2018","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1139\/z65-092","article-title":"The effect of nutrient intake on the development and the egg production of Rhodnius prolixus St\u00e5hl (Hemiptera: Reduviidae)","volume":"43","author":"Friend W.G.","year":"1965","journal-title":"Canadian Journal of Zoology"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"6469","DOI":"10.1073\/pnas.97.12.6469","article-title":"Cockroach diuretic hormones: characterization of a calcitonin\u2010like peptide in insects","volume":"97","author":"Furuya K.","year":"2000","journal-title":"Proceedings of the National Academy of Sciences USA"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1016\/j.regpep.2012.05.001","article-title":"Mini\u2010review: the evolution of neuropeptide signaling","volume":"177","author":"Grimmelikhuijzen C.J.","year":"2012","journal-title":"Regulatory Peptides"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jmor.1051380102","article-title":"A cytological study of the ovary of Rhodnius prolixus. III. Cytoarchitecture and development of the trophic chamber","volume":"138","author":"Huebner E.","year":"1972","journal-title":"Journal of Morphology"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/BF00214685","article-title":"Immunoreactive material resembling vertebrate neuropeptides in the corpus cardiacum and corpus allatum of the insect Leucophaea maderae","volume":"225","author":"Hansen B.L.","year":"1982","journal-title":"Cell and Tissue Research"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1101\/gr.169901","article-title":"Neuropeptides and neuropeptide receptors in the Drosophila melanogaster genome","volume":"11","author":"Hewes R.S.","year":"2001","journal-title":"Genome Research"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1242\/jeb.00818","article-title":"Drosophila CG8422 encodes a functional diuretic hormone receptor","volume":"207","author":"Johnson E.C.","year":"2004","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.peptides.2011.04.009","article-title":"The effects of linear and cyclic analogs of Locmi\u2010DH, Dippu\u2010DH46 and Dippu\u2010DH31 on appetitive behavior in Locusta migratoria","volume":"34","author":"Kaskani C.","year":"2012","journal-title":"Peptides"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","first-page":"14211","DOI":"10.1073\/pnas.0603459103","article-title":"Central peptidergic ensembles associated with organization of an innate behavior","volume":"103","author":"Kim Y.J.","year":"2006","journal-title":"Proceedings of the National Academy of Sciences USA"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/0016-6480(82)90047-8","article-title":"Ovarian motility in mated Rhodnius prolixus requires an intact cerebral neurosecretory system","volume":"48","author":"Kriger F.L.","year":"1982","journal-title":"General and Comparative Endocrinology"},{"key":"e_1_2_8_21_1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1472-6793-10-14","article-title":"Peristalsis in the junction region of the Drosophila larval midgut is modulated by DH31 expressing enteroendocrine cells","volume":"10","author":"LaJeunesse D.R.","year":"2010","journal-title":"BMC Physiology"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.ygcen.2022.114030","article-title":"The hormonal and neural control of egg production in the historically important model insect, Rhodnius prolixus: a review, with new insights in this post\u2010genomic era","volume":"321","author":"Lange A.B.","year":"2022","journal-title":"General and Comparative Endocrinology"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0008516","article-title":"What happens after a blood meal? A transcriptome response from the main tissues involved in egg production in Rhodnius prolixus, an insect vector of Chagas disease","volume":"14","author":"Leyria J.","year":"2020","journal-title":"PLoS Neglected Tropical Disease"},{"key":"e_1_2_8_24_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3390\/ijms24010007","article-title":"Crosstalk between nutrition, insulin, juvenile hormone, and ecdysteroid signaling in the classical insect model, Rhodnius prolixus","volume":"24","author":"Leyria J.","year":"2022","journal-title":"International Journal of Molecular Sciences"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.mce.2024.112211","article-title":"Endocrine factors modulating vitellogenesis and oogenesis in insects: an update","volume":"587","author":"Leyria J.","year":"2024","journal-title":"Molecular and Cellular Endocrinology"},{"key":"e_1_2_8_26_1","first-page":"1","volume-title":"Advances in Experimental Medicine and Biology","author":"Leyria J.","year":"2024"},{"key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real\u2010time quantitative PCR and the 2\u2013\u0394\u0394CT method","volume":"25","author":"Livak K.J.","year":"2001","journal-title":"Methods (San Diego, Calif.)"},{"key":"e_1_2_8_28_1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1242\/jeb.51.1.71","article-title":"Secretion by the Malpighian tubules of Rhodnius. The movements of ions and water","volume":"51","author":"Maddrell S.H.P.","year":"1969","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_8_29_1","doi-asserted-by":"crossref","DOI":"10.1098\/rsob.220174","article-title":"Endocrine cybernetics: neuropeptides as molecular switches in behavioural decisions","volume":"12","author":"N\u00e4ssel D.R.","year":"2022","journal-title":"Open Biology"},{"key":"e_1_2_8_30_1","first-page":"250","article-title":"Peptides and serotonin control feeding\u2010related events in Rhodnius prolixus","volume":"1","author":"Orchard I.","year":"2009","journal-title":"Frontiers in Bioscience"},{"key":"e_1_2_8_31_1","first-page":"225","volume-title":"Hormonal control of diuresis in insects, Advances in Invertebrate(Neuro)Endocrinology, Apple Academic Press","author":"Orchard I.","year":"2020"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2021.722487","article-title":"Fluid secretion by Malpighian tubules of Rhodnius prolixus: neuroendocrine control with new insights from a transcriptome analysis","volume":"12","author":"Orchard I.","year":"2021","journal-title":"Frontiers in Endocrinology"},{"key":"e_1_2_8_33_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0001896","article-title":"Mapping peptidergic cells in Drosophila: where DIMM fits in","volume":"3","author":"Park D.","year":"2008","journal-title":"PLoS ONE"},{"key":"e_1_2_8_34_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-022-18384-5","article-title":"Role of Methoprene\u2010tolerant in the regulation of oogenesis in Dipetalogaster maxima","volume":"12","author":"Ramos F.O.","year":"2022","journal-title":"Scientific Reports"},{"key":"e_1_2_8_35_1","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real\u2010time PCR data by the comparative C\n                  T method","volume":"3","author":"Schmittgen T.D.","year":"2008","journal-title":"Nature Protocols"},{"key":"e_1_2_8_36_1","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.peptides.2013.04.003","article-title":"The female reproductive system of the kissing bug, Rhodnius prolixus: arrangement of muscles, distribution and myoactivity of two endogenous FMRFamide\u2010like peptides","volume":"53","author":"Sedra L.","year":"2014","journal-title":"Peptides"},{"key":"e_1_2_8_37_1","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1074\/jbc.M115.664003","article-title":"Evidence for conservation of the calcitonin superfamily and activity\u2010regulating mechanisms in the basal chordate Branchiostoma floridae: insights into the molecular and functional evolution in chordates","volume":"291","author":"Sekiguchi T.","year":"2016","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_8_38_1","doi-asserted-by":"crossref","DOI":"10.1590\/0074-02760200479","article-title":"Chagas disease in the context of the 2030 agenda: global warming and vectors","volume":"117","author":"Souza R.D.C.M.D.","year":"2022","journal-title":"Memorias do Instituto Oswaldo Cruz"},{"key":"e_1_2_8_39_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.peptides.2004.08.025","article-title":"Presence and activity of a Dippu\u2010DH31\u2010like peptide in the blood\u2010feeding bug, Rhodnius prolixus","volume":"26","author":"Te Brugge V.A.","year":"2005","journal-title":"Peptides"},{"key":"e_1_2_8_40_1","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1242\/jeb.013771","article-title":"Amino acid sequence and biological activity of a calcitonin\u2010like diuretic hormone (DH31) from Rhodnius prolixus","volume":"211","author":"Te Brugge V.A.","year":"2008","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_8_41_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/BF00502085","article-title":"Immunocytochemical localization of peptidergic neurons and neurosecretory cells in the neuroendocrine system of the Colorado potato beetle with antisera to vertebrate regulatory peptides","volume":"82","author":"Veenstra J.A.","year":"1985","journal-title":"Histochemistry"},{"key":"e_1_2_8_42_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ygcen.2015.04.018","article-title":"Genomic and functional characterization of a methoprene\u2010tolerant gene in the kissing\u2010bug Rhodnius prolixus","volume":"216","author":"Villalobos\u2010Sambucaro M.J.","year":"2015","journal-title":"General and Comparative Endocrinology"},{"key":"e_1_2_8_43_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.ygcen.2023.114358","article-title":"Allatotropic peptides modulate muscle contraction of the female reproductive system in Rhodnius prolixus (ST\u00c4L)","volume":"343","author":"Villalobos\u2010Sambucaro M.J.V.","year":"2023","journal-title":"General and Comparative Endocrinology"},{"key":"e_1_2_8_44_1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1242\/jcs.s2-79.313.91","article-title":"The function of the corpus allatum in the growth and reproduction of Rhodnius prolixus (Hemiptera)","volume":"2","author":"Wigglesworth V.B.","year":"1936","journal-title":"Journal of Cell Science"},{"key":"e_1_2_8_45_1","unstructured":"WHO. (2025)Chagas disease (American trypanosomiasis).https:\/\/www.who.int\/health\u2010topics\/chagas\u2010disease#tab=tab_1."},{"key":"e_1_2_8_46_1","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1016\/j.ibmb.2012.06.006","article-title":"Calcitonin\u2010like diuretic hormones in insects","volume":"42","author":"Zandawala M.","year":"2012","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"e_1_2_8_47_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.mce.2010.08.012","article-title":"Isolation and characterization of the cDNA encoding DH31 in the kissing bug, Rhodnius prolixus","volume":"331","author":"Zandawala M.","year":"2011","journal-title":"Molecular and Cellular Endocrinology"},{"key":"e_1_2_8_48_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0082466","article-title":"Isolation and functional characterization of calcitonin\u2010like diuretic hormone receptors in Rhodnius prolixus","volume":"8","author":"Zandawala M.","year":"2013","journal-title":"PLoS ONE"}],"container-title":["Insect Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-7917.70211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T11:35:37Z","timestamp":1767872137000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-7917.70211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,8]]},"references-count":47,"alternative-id":["10.1111\/1744-7917.70211"],"URL":"https:\/\/doi.org\/10.1111\/1744-7917.70211","archive":["Portico"],"relation":{},"ISSN":["1672-9609","1744-7917"],"issn-type":[{"value":"1672-9609","type":"print"},{"value":"1744-7917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,8]]},"assertion":[{"value":"2025-07-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1744-7917.70211"}}