{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T19:03:41Z","timestamp":1788807821789,"version":"build-2803163510"},"reference-count":76,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170511"],"award-info":[{"award-number":["32170511"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    V\u2010ATPases are crucial for animal development and survival, but their functions in fertility are largely unknown. Here, we found that knockdown of\n                    <jats:italic>VhaM9.7\u2010d<\/jats:italic>\n                    in germ cells induced complete sterility in male\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    , but had no effects on female fertility. Depletion of\n                    <jats:italic>VhaM9.7\u2010d<\/jats:italic>\n                    did not severely impair spermatogenesis, as the mature sperm appeared in the seminal vesicles (SVs) of the testes. However, the sperm released from the SVs of the\n                    <jats:italic>VhaM9.7\u2010d<\/jats:italic>\n                    \u2010knockdown males rapidly lost their motility and were unable to move over long distances. These sperm could be transferred to the female's uterus during copulation, but failed to be stored in the seminal receptacle (SR) and fertilize the egg. Tandem mass tag (TMT) proteomic analyses of SVs, including their contents, identified 434 differentially expressed proteins (DEPs) when comparing the control group to the\n                    <jats:italic>VhaM9.7\u2010d<\/jats:italic>\n                    \u2010knockdown group. Many downregulated proteins were enriched in phagosome and oxidative phosphorylation (OxPHOS) pathways. Subsequent experiments, encompassing the LysoSensor Probe assay, CMXRos staining, and ATP measurement, confirmed that the knockdown of\n                    <jats:italic>VhaM9.7\u2010d<\/jats:italic>\n                    significantly disrupted phagolysosomal and mitochondrial functions, leading to diminished acidity, heightened levels of reactive oxygen species (ROS), and a decrease in both mitochondrial membrane potential and ATP contents in the SVs. These results suggest that\n                    <jats:italic>VhaM9.7\u2010d<\/jats:italic>\n                    plays an essential role in maintaining the homeostasis of sperm energy metabolism by regulating phagolysosomal activity and mitochondrial OxPHOS. Our data provide valuable insights for the further study of the mechanisms of related diseases such as human asthenospermia.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70212","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T11:12:11Z","timestamp":1767870731000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["V\u2010ATPase subunit M9.7\u2010d is essential for sperm motility in\n                    <i>Drosophila melanogaster<\/i>"],"prefix":"10.1111","author":[{"given":"Si\u2010Ying","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Pesticide &amp; Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences Central China Normal University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao\u2010Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide &amp; Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences Central China Normal University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide &amp; Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences Central China Normal University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo\u2010Shuo","family":"Ren","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide &amp; Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences Central China Normal University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide &amp; Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences Central China Normal University  Wuhan China"},{"name":"School of Life Sciences Shanghai Normal University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1823-373X","authenticated-orcid":false,"given":"Yu\u2010Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide &amp; Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences Central China Normal University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1152\/physiolgenomics.00233.2004","article-title":"Genome\u2010wide survey of V\u2010ATPase genes in Drosophila reveals a conserved renal phenotype for lethal alleles","volume":"22","author":"Allan A.K.","year":"2005","journal-title":"Physiological Genomics"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"4131","DOI":"10.1038\/sj.emboj.7601272","article-title":"Epididymal expression of the forkhead transcription factor Foxi1 is required for male fertility","volume":"25","author":"Blomqvist S.R.","year":"2006","journal-title":"The EMBO Journal"},{"key":"e_1_2_7_4_1","first-page":"318","article-title":"Regulation of luminal acidification by the V\u2010ATPase","volume":"28","author":"Breton S.","year":"2013","journal-title":"Physiology (Bethesda, 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