{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T21:45:14Z","timestamp":1768859114219,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Disruption of reproduction is an important pest control tactics for management of high\u2010fecundity pests like\n                    <jats:italic>Helicoverpa armigera<\/jats:italic>\n                    (H\u00fcbner). In this study, we investigated the role of the testis\u2010biased miR\u2010252a\u20105p in regulating gonad development and reproduction. Through a combination of bioinformatics prediction, dual luciferase reporter assays, functional injections (agomir, antagomir, and siRNA), and phenotypic evaluations, we identified\n                    <jats:italic>OVOL<\/jats:italic>\n                    as a key target gene of miR\u2010252a\u20105p. We found that miR\u2010252a\u20105p negatively regulates\n                    <jats:italic>OVOL<\/jats:italic>\n                    expression. Injection of miR\u2010252a\u20105p agomir or\n                    <jats:italic>OVOL<\/jats:italic>\n                    siRNA into newly emerged females delayed egg maturation and reduced hatching rates. In contrast, inhibiting miR\u2010252a\u20105p or knocking down\n                    <jats:italic>OVOL<\/jats:italic>\n                    in larval stages did not affect testis development or fertility. These results demonstrate that miR\u2010252a\u20105p specifically targets\n                    <jats:italic>OVOL<\/jats:italic>\n                    to modulate ovarian development and reproductive success in\n                    <jats:italic>H. armigera<\/jats:italic>\n                    . Both miR\u2010252a\u20105p and\n                    <jats:italic>OVOL<\/jats:italic>\n                    represent promising targets for reproduction\u2010based control of this and other lepidopteran pests.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70239","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T08:44:35Z","timestamp":1768812275000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["miR\u2010252a\u20105p targets\n                    <i>OVOL<\/i>\n                    to regulate ovary development and egg viability (hatchability) in\n                    <i>Helicoverpa armigera<\/i>"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3557-7278","authenticated-orcid":false,"given":"Zhongyuan","family":"Deng","sequence":"first","affiliation":[{"name":"Zhengzhou Research Base, State Key Laboratory of Cotton Bio\u2010breeding and Integrated Utilization, School of Agriculture and Biomanufacturing Zhengzhou University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leyao","family":"Li","sequence":"additional","affiliation":[{"name":"Zhengzhou Research Base, State Key Laboratory of Cotton Bio\u2010breeding and Integrated Utilization, School of Agriculture and Biomanufacturing Zhengzhou University  Zhengzhou China"},{"name":"School of Life Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liying","family":"Fang","sequence":"additional","affiliation":[{"name":"Zhengzhou Research Base, State Key Laboratory of Cotton Bio\u2010breeding and Integrated Utilization, School of Agriculture and Biomanufacturing Zhengzhou University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinzhi","family":"Ni","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS, Crop Genetics and Breeding Research Unit University of Georgia\u2014Tifton Campus  Tifton Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianchun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Entomology and BIO5 Institute University of Arizona  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-015-9880-x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep02292"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.03.005"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-031616-034925"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/g3journal\/jkae252","article-title":"Female germline expression of OVO transcription factor bridges Drosophila 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