{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:41:49Z","timestamp":1787013709396,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272542"],"award-info":[{"award-number":["32272542"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Carbon dioxide (CO\n                    <jats:sub>2<\/jats:sub>\n                    ) is a critical cue for\n                    <jats:italic>Holotrichia oblita<\/jats:italic>\n                    larvae (white grubs), a widely distributed soil\u2010dwelling pest, in locating host plant roots. However, the molecular mechanisms underlying the perception of CO\n                    <jats:sub>2<\/jats:sub>\n                    in white grubs remain elusive. Here, our study demonstrated that CO\n                    <jats:sub>2<\/jats:sub>\n                    could induce electrophysiological and behavioral responses in third\u2010instar larvae. Twelve to fourteen S4 sensilla and one digitiform organ potentially involved in CO\n                    <jats:sub>2<\/jats:sub>\n                    detection were observed on the antenna and maxillary palp of\n                    <jats:italic>H. oblita<\/jats:italic>\n                    larvae, respectively. Three gustatory receptor (GR) genes\u2014\n                    <jats:italic>HoblGR1<\/jats:italic>\n                    ,\n                    <jats:italic>HoblGR2<\/jats:italic>\n                    , and\n                    <jats:italic>HoblGR3<\/jats:italic>\n                    \u2014were cloned and were predominantly expressed in the antennae and maxillary palps of third\u2010instar larvae. The two\u2010electrode voltage clamp (TEVC) recording system demonstrated that oocytes co\u2010expressing HoblGR1 and HoblGR3, or HoblGR1, HoblGR2, and HoblGR3, gave robust responses to CO\n                    <jats:sub>2<\/jats:sub>\n                    . Additionally, silencing of\n                    <jats:italic>HoblGR1<\/jats:italic>\n                    or\n                    <jats:italic>HoblGR3<\/jats:italic>\n                    for 72 h significantly reduced the electrophysiological and behavioral responses of white grubs to commercial CO\n                    <jats:sub>2<\/jats:sub>\n                    as well as root\u2010emitted CO\n                    <jats:sub>2<\/jats:sub>\n                    . Our work reveals that HoblGR1 and HoblGR3 are indispensable and sufficient for CO\n                    <jats:sub>2<\/jats:sub>\n                    sensing in\n                    <jats:italic>H. oblita<\/jats:italic>\n                    larvae, and this finding may aid in the development of CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010based trapping strategies for white grubs.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70261","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:56:40Z","timestamp":1772981800000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mechanisms of CO\n                    <sub>2<\/sub>\n                    sensing mediated by gustatory receptors in\n                    <i>Holotrichia oblita<\/i>\n                    larvae"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1206-7395","authenticated-orcid":false,"given":"Ertao","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanhuan","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafei","family":"Qu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Plant Protection Yangzhou University  Yangzhou Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenglong","family":"Cui","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kebin","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences  Beijing 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