{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T09:02:47Z","timestamp":1773651767211,"version":"3.50.1"},"reference-count":87,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The Asian tiger mosquito (\n                    <jats:italic>Aedes albopictus<\/jats:italic>\n                    ) is a rapidly expanding invasive species and a global vector of arboviruses. Its transmission depends on the female's blood meal\u2010driven reproductive cycle. Although mosquito PIWI\u2010interacting RNAs (piRNAs) research has predominantly focused on antiviral immunity, their roles in reproduction remain poorly understood. Here we profiled sex\u2010biased piRNA expression in\n                    <jats:italic>Ae. albopictus<\/jats:italic>\n                    and identified a female\u2010enriched piRNA, AalpiRNA\u201018529. This piRNA accumulates in pre\u2010vitellogenic ovaries and is rapidly downregulated after a blood meal. Gain\u2010of\u2010function experiments showed that AalpiRNA\u201018529 mimics impair follicle development, reduces fecundity and egg hatching, and disrupts nurse\u2010cell apoptosis required for oocyte maturation. Mechanistically, AalpiRNA\u201018529 represses\n                    <jats:italic>Gadd45a<\/jats:italic>\n                    , a conserved stress\u2010response regulator.\n                    <jats:italic>Gadd45a<\/jats:italic>\n                    knockdown phenocopies AalpiRNA\u201018529 gain\u2010of\u2010function, leading to reduced phosphorylation of c\u2010Jun N\u2010terminal kinase (JNK) and thus indicating defective activation of the\n                    <jats:italic>Gadd45a<\/jats:italic>\n                    \u2013JNK apoptotic axis. Collectively, our findings reveal a piRNA\u2010mediated pathway that controls ovarian development in\n                    <jats:italic>Ae. albopictus<\/jats:italic>\n                    during vitellogenesis and highlight the AalpiRNA\u201018529\u2013\n                    <jats:italic>Gadd45a<\/jats:italic>\n                    \u2013JNK cascade as a potential molecular target for genetic strategies to disrupt mosquito reproduction.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70262","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T08:21:25Z","timestamp":1773649285000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An ovary\u2010enriched piRNA controls ovarian development via\n                    <i>Gadd45a<\/i>\n                    \u2013JNK\u2010mediated nurse\u2010cell apoptosis in the arbovirus vector\n                    <i>Aedes albopictus<\/i>"],"prefix":"10.1111","author":[{"given":"Lu","family":"Yang","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research Key Laboratory of Infectious Diseases Research in South China (Southern Medical University), Ministry of Education, School of Public Health, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonghui","family":"Gao","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research Key Laboratory of Infectious Diseases Research in South China (Southern Medical University), Ministry of Education, School of Public Health, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulan","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research Key Laboratory of Infectious Diseases Research in South China (Southern Medical University), Ministry of Education, School of Public Health, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuyi","family":"Ren","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research Key Laboratory of Infectious Diseases Research in South China (Southern Medical University), Ministry of Education, School of Public Health, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongzheng","family":"Lu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research Key Laboratory of Infectious Diseases Research in South China (Southern Medical University), Ministry of Education, School of Public Health, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifan","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research Key Laboratory of Infectious Diseases Research in South China (Southern Medical University), Ministry of Education, School of Public Health, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peiwen","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangzhou Center for Disease Control and Prevention  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khadija","family":"Batool","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute Cornell University  Ithaca New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7927-691X","authenticated-orcid":false,"given":"Xiao\u2010Guang","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research Key Laboratory of Infectious Diseases Research in South China (Southern Medical University), Ministry of Education, School of Public Health, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7145-9922","authenticated-orcid":false,"given":"Jianxia","family":"Tang","sequence":"additional","affiliation":[{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key Laboratory Jiangsu Institute of Parasitic Diseases  Wuxi Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7933-4534","authenticated-orcid":false,"given":"Jinbao","family":"Gu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research Key Laboratory of Infectious Diseases Research in South China (Southern Medical University), Ministry of Education, School of Public Health, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.3389\/finsc.2022.1073308","article-title":"Characterizing oogenesis and programmed cell death in the eastern tree hole mosquito Aedes (Protomacleaya) triseriatus","volume":"2","author":"Airs P.M.","year":"2023","journal-title":"Frontiers in Insect 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