{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T18:48:45Z","timestamp":1774810125170,"version":"3.50.1"},"reference-count":122,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T00:00:00Z","timestamp":1774742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T00:00:00Z","timestamp":1774742400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"publisher","award":["NE\/V012053\/1"],"award-info":[{"award-number":["NE\/V012053\/1"]}],"id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/K003240\/1"],"award-info":[{"award-number":["BB\/K003240\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/S002928\/"],"award-info":[{"award-number":["BB\/S002928\/"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["109356\/Z\/15\/Z"],"award-info":[{"award-number":["109356\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["WT097835MF"],"award-info":[{"award-number":["WT097835MF"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["WT101650MA"],"award-info":[{"award-number":["WT101650MA"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Microbiome have been shown to play an important role in host susceptibility to infections in some hosts. However, less is known about whether microbiota\u2010mediated effects are consistent across host species, as our understanding of such interactions may be affected by publication bias. Following on from a large study of 36 species of Drosophilidae challenged with four bacterial pathogens, we identified two candidate host species that might have protective microbiomes based on low susceptibility and high abundance of culturable microbiota; we selected two other host species for comparison. We tested whether germ\u2010reduced flies, and flies with natural or re\u2010constituted microbiomes varied in their susceptibility to systemic infection with two bacterial pathogens (\n                    <jats:italic>Providencia rettgeri<\/jats:italic>\n                    and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ) and one viral pathogen (Drosophila C Virus). The composition and abundance of the bacterial microbiota varied between host species and microbiome treatments. We found an overall interaction between host species and pathogen type, confirming previous work that host species vary in their susceptibility in a pathogen specific manner. Similarly, we found that microbiome treatments had differing effects on host survival among host species, although some effect sizes tended to be small. In\n                    <jats:italic>D. putrida<\/jats:italic>\n                    individuals with manipulated microbiomes showed increased susceptibility to all pathogens tested; in other hosts altered susceptibility was pathogen dependent. While there are always challenges to manipulating microbiomes, especially across multiple host species, our results indicate that host microbiota play limited roles on survival in systemic infection in these four species. This work demonstrates that caution is required when generalizing about potential beneficial impact of microbiomes.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70275","type":"journal-article","created":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T18:35:13Z","timestamp":1774809313000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of the microbiome on pathogen susceptibility across four Drosophilidae species"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7272-7205","authenticated-orcid":false,"given":"Hongbo","family":"Sun","sequence":"first","affiliation":[{"name":"Centre for Ecology &amp; Conservation, Faculty of Environment Science, and Economy, University of Exeter  Penryn UK"},{"name":"CNRS UPR9022, Institut de Biologie Mol\u00e9culaire et Cellulaire Universit\u00e9 de Strasbourg  Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6936-1697","authenticated-orcid":false,"given":"Ben","family":"Longdon","sequence":"additional","affiliation":[{"name":"Centre for Ecology &amp; Conservation, Faculty of Environment Science, and Economy, University of Exeter  Penryn UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3730-0985","authenticated-orcid":false,"given":"Ben","family":"Raymond","sequence":"additional","affiliation":[{"name":"Centre for Ecology &amp; Conservation, Faculty of Environment Science, and Economy, University of Exeter  Penryn UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1002\/bimj.200410141","article-title":"A version of the EM algorithm for proportional hazard model with random effects","volume":"47","author":"Abrahantes J.C.","year":"2005","journal-title":"Biometrical Journal"},{"key":"e_1_2_12_3_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2024.105221","article-title":"Evidence for immune priming specificity and cross\u2010protection against sympatric and allopatric Vibrio splendidus strains in the oyster Magalana (Crassostrea) gigas","volume":"159","author":"Arfatahery N.","year":"2024","journal-title":"Developmental & Comparative Immunology"},{"key":"e_1_2_12_4_1","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2305649120","article-title":"Resistance to host antimicrobial peptides mediates resilience of gut commensals during infection and aging in Drosophila","volume":"120","author":"Arias\u2010Rojas A.","year":"2023","journal-title":"Proceedings of the National Academy of Sciences USA"},{"key":"e_1_2_12_5_1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/insr.12214","article-title":"A tutorial on multilevel survival analysis: methods, models and applications","volume":"85","author":"Austin P.C.","year":"2017","journal-title":"International Statistical Review"},{"key":"e_1_2_12_6_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2024.114087","article-title":"Microbiome\u2010derived acidity protects against microbial invasion in Drosophila","volume":"43","author":"Barron A.J.","year":"2024","journal-title":"Cell Reports"},{"key":"e_1_2_12_7_1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1186\/s40168-020-00875-0","article-title":"Microbiome definition re\u2010visited: old concepts and new challenges","volume":"8","author":"Berg G.","year":"2020","journal-title":"Microbiome"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02199\u201017"},{"key":"e_1_2_12_9_1","doi-asserted-by":"crossref","unstructured":"Blum J.E. 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