{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T10:43:11Z","timestamp":1776681791491,"version":"3.51.2"},"reference-count":53,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFD1401200"],"award-info":[{"award-number":["2024YFD1401200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Crustacean cardioactive peptide receptor (CCAPR), a G protein\u2010coupled receptor, is critical for pupal ecdysis in\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    and\n                    <jats:italic>Acyrthosiphon pisum<\/jats:italic>\n                    , both of which possess a single\n                    <jats:italic>ccapr<\/jats:italic>\n                    gene. In this study, we identified two\n                    <jats:italic>ccapr<\/jats:italic>\n                    genes in\n                    <jats:italic>Henosepilachna vigintioctomaculata<\/jats:italic>\n                    , a potato\u2010defoliating ladybird widely distributed in Asia, and found that the two receptors have diverged functionally in their requirement for pupal ecdysis. Bayesian phylogenetic analysis recovered well\u2010supported within\u2010lineage relationships and placed HvCCAPR1 and HvCCAPR2 as a sister pair within the coleopteran clade. Spatiotemporal expression analysis showed that\n                    <jats:italic>Hvccapr2<\/jats:italic>\n                    was upregulated immediately before the onset of pupal ecdysis and exhibited a stronger bias toward neural and muscular tissues. Injection of\n                    <jats:italic>in vitro<\/jats:italic>\n                    \u2010synthesized dsRNA or oral administration of bacterially expressed dsRNA targeting the common region shared by both genes, as well as ds\n                    <jats:italic>ccapr2<\/jats:italic>\n                    but not ds\n                    <jats:italic>ccapr1<\/jats:italic>\n                    , impaired larval\u2013pupal transition. Moreover, knockdown of\n                    <jats:italic>Hvccapr1<\/jats:italic>\n                    +\n                    <jats:italic>Hvccapr2<\/jats:italic>\n                    or\n                    <jats:italic>Hvccapr2<\/jats:italic>\n                    , but not\n                    <jats:italic>Hvccapr1<\/jats:italic>\n                    , markedly prolonged the durations of ecdysis and postecdysis behavioral sequences. As a consequence, most\n                    <jats:italic>Hvccapr1<\/jats:italic>\n                    +\n                    <jats:italic>Hvccapr2<\/jats:italic>\n                    and\n                    <jats:italic>Hvccapr2<\/jats:italic>\n                    RNAi prepupae failed to shed the old larval cuticle. In contrast, pupal cuticles beneath the retained larval exoskeleton became pigmented in developmentally arrested\n                    <jats:italic>Hvccapr1<\/jats:italic>\n                    +\n                    <jats:italic>Hvccapr2<\/jats:italic>\n                    and\n                    <jats:italic>Hvccapr2<\/jats:italic>\n                    RNAi beetles. Together, these findings demonstrate that CCAPR2, rather than CCAPR1, plays the predominant role in coordinating pupal ecdysis behavior in\n                    <jats:italic>H. vigintioctomaculata<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70289","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T09:42:38Z","timestamp":1776678158000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CCAP receptor 2, rather than CCAPR1, functions in pupal ecdysis in the 28\u2010spotted larger potato ladybird"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1514-5566","authenticated-orcid":false,"given":"Yi\u2010Kuan","family":"Wu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing 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of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7449-8433","authenticated-orcid":false,"given":"Guo\u2010Qing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing 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