{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:01:45Z","timestamp":1790233305422,"version":"4.1.0"},"reference-count":73,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32572833"],"award-info":[{"award-number":["32572833"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Horizontal gene transfer (HGT) has contributed substantially to the evolution of arthropod genomes, yet the functional significance of many horizontally acquired genes remains poorly understood. The hawthorn spider mite,\n                    <jats:italic>Amphitetranychus viennensis<\/jats:italic>\n                    , is a devastating agricultural pest whose high fecundity and overwintering diapause afford its exceptional ecological resilience. Through a genome\u2010wide screen, we identified 37 high\u2010confidence horizontally transferred genes (HTGs) in\n                    <jats:italic>A. viennensis<\/jats:italic>\n                    . Among these candidates, we prioritized\n                    <jats:italic>AvPBL<\/jats:italic>\n                    , a gene encoding pantothenate\u2010\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010alanine ligase, for functional characterization because it controls the rate\u2010limiting step of a distinctly non\u2010metazoan pantothenic acid (vitamin B5) biosynthesis pathway. RNAi\u2010mediated suppression of\n                    <jats:italic>AvPBL<\/jats:italic>\n                    significantly reduced transcript abundance and endogenous pantothenic acid levels, triggering a 23.7% reduction in cumulative fecundity and severely compromising the mites\u2019 ability to enter winter diapause. Importantly, exogenous pantothenic acid supplementation rescued these reproductive and diapause defects, directly linking the observed phenotypes to the disruption of pantothenic acid biosynthesis. Our results demonstrate that the horizontally transferred bacterial gene\n                    <jats:italic>AvPBL<\/jats:italic>\n                    has been functionally integrated into the endogenous metabolic network of\n                    <jats:italic>A. viennensis<\/jats:italic>\n                    , playing a critical role in vitamin B5 biosynthesis, reproduction, and diapause regulation. These findings provide direct evidence that horizontally acquired metabolic genes can shape key life\u2010history traits and drive adaptive evolution in arthropods.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70356","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:01:02Z","timestamp":1789624862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A horizontally transferred bacterial gene for pantothenic acid biosynthesis regulates diapause and reproduction in the spider mite\n                    <i>Amphitetranychus viennensis<\/i>"],"prefix":"10.1111","author":[{"given":"Meibin","family":"Liu","sequence":"first","affiliation":[{"name":"College of Plant Protection Shanxi Agricultural University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shanxi Agricultural University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yikang","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shanxi Agricultural University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shanxi Agricultural University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meifeng","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shanxi Agricultural University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shanxi Agricultural University  Taiyuan 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