{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:00:31Z","timestamp":1790146831491,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772676"],"award-info":[{"award-number":["31772676"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Science"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Spider silk possesses excellent material properties and demonstrates significant potential for application in the fields of biomedicine and the military. However, the cannibalistic and territorial nature of spiders precludes farm\u2010based mass production of spider silk. To overcome this limitation, we employed targeted genome editing to enable the biosynthetic production of spider silk proteins in silkworm silk gland bioreactors. Specifically, one fold and double repeats of the\n                    <jats:italic>cre\u2010MaSp1<\/jats:italic>\n                    gene from the black widow spider were inserted into the 3' end of the silkworm\n                    <jats:italic>FibL<\/jats:italic>\n                    gene via site\u2010directed integration using transcription activator\u2010like effector nuclease (TALEN)\u2010mediated homology\u2010directed repair. By introducing the T2A self\u2010cleavage peptide, which allows normal cross\u2010linking of intact FibL to FibH, cre\u2010MaSp1 are efficiently secreted into the cocoon shell, while mitigating the damage to cocoon economic traits caused by previous FibL direct fusion. The tensile test results showed that the expression of the\n                    <jats:italic>cre\u2010MaSp1<\/jats:italic>\n                    gene was beneficial for changing the maximum strain of the resulting transgenic silk fibers. Notably, as the number of repeats increases, the maximum strain and stress of the transgenic silk shows a rising trend, pointing the way toward improved mechanical properties of the silk. Furthermore, AlphaFold 3 prediction and Fourier\u2010transform infrared (FTIR) spectroscopic analyses indicated that the transgenic silk contained a higher content of\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010helix and random coil structures than wild\u2010type silk. RNA sequencing results further suggested that the integration of the\n                    <jats:italic>cre\u2010MaSp1<\/jats:italic>\n                    gene into the\n                    <jats:italic>FibL<\/jats:italic>\n                    locus of the silkworm interfered with ribosome biogenesis. This study provides a promising strategy for the biofabrication of silkworm\u2013spider composite silk.\n                  <\/jats:p>","DOI":"10.1111\/1744-7917.70362","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:09:01Z","timestamp":1790143741000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering silkworm\u2013spider composite silk via the fibroin light chain fusion system in\n                    <i>Bombyx mori<\/i>"],"prefix":"10.1111","author":[{"given":"Shihua","family":"Yu","sequence":"first","affiliation":[{"name":"Institute of Silkworm and Bee Research, College of Animal Sciences Zhejiang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Cui","sequence":"additional","affiliation":[{"name":"Intelligent Optimization and Bioinformatics Lab, College of Information Engineering Zhejiang University of Technology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Dentistry Nanchang University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Dai","sequence":"additional","affiliation":[{"name":"Suposik Bioscience Technologies Ltd.  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3917-5222","authenticated-orcid":false,"given":"Xiaogang","family":"Ye","sequence":"additional","affiliation":[{"name":"Institute of Silkworm and Bee Research, College of Animal Sciences Zhejiang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangping","family":"Dai","sequence":"additional","affiliation":[{"name":"Institute of Silkworm and Bee Research, College of Animal Sciences Zhejiang University Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Intelligent Optimization and Bioinformatics Lab, College of Information Engineering Zhejiang University of Technology Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0087-8839","authenticated-orcid":false,"given":"Boxiong","family":"Zhong","sequence":"additional","affiliation":[{"name":"Institute of Silkworm and Bee Research, College of Animal Sciences Zhejiang University Hangzhou China"},{"name":"Suposik Bioscience Technologies Ltd.  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson J.","year":"2024","journal-title":"Nature"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3390\/insects13010028","article-title":"Advances in editing silkworms (Bombyx mori) genome by using the CRISPR\u2010Cas system","volume":"13","author":"Baci G.M.","year":"2021","journal-title":"Insects"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1126\/science.1178811","article-title":"Breaking the code of DNA binding specificity of TAL\u2010type III effectors","volume":"326","author":"Boch 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