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Using a proteomics approach, we aimed to identify biological markers that predict the response to LDL\u2010A in patients with steroid\u2010resistant nephrotic syndrome (SRNS). Identification of plasma proteins bound to the dextran\u2010sulfate column at the first session of LDL\u2010A was determined by mass spectrometry. To investigate biological factors associated with the response to LDL\u2010A, we compared profiles of column\u2010bound proteins between responders (defined by more than 50% reduction of proteinuria after the treatment) and non\u2010responders by 2\u2010dimensional gel electrophoresis (2\u2010DE) coupled to mass spectrometry in seven patients with SRNS. Evaluation of proteins adsorbed to LDL\u2010A column in patients with SRNS revealed the identity of 62 proteins, which included apolipoproteins, complement components, and serum amyloid P\u2010component (SAP). Comparative analysis of the column\u2010bound proteins between responders and non\u2010responders by 2\u2010DE demonstrated that apolipoprotein E (APOE) and SAP levels were increased in non\u2010responders as compared with responders. These results were confirmed by western blotting. Moreover, serum levels of APOE and SAP were significantly higher in the non\u2010responder group than in the responder group by ELISA. Our data provide comprehensive analysis of proteins adsorbed by LDL\u2010A in SRNS, and demonstrate that the serum levels of APOE and SAP may be used to predict the response to LDL\u2010A in these patients.<\/jats:p>","DOI":"10.1111\/1744-9987.12356","type":"journal-article","created":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T12:10:39Z","timestamp":1452773439000},"page":"174-182","source":"Crossref","is-referenced-by-count":3,"title":["Proteomics Approach Identifies Factors Associated With the Response to Low\u2010Density Lipoprotein Apheresis Therapy in Patients With Steroid\u2010Resistant Nephrotic Syndrome"],"prefix":"10.1111","volume":"20","author":[{"given":"Emiko","family":"Kuribayashi\u2010Okuma","sequence":"first","affiliation":[{"name":"Departments of Internal Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Shibata","sequence":"additional","affiliation":[{"name":"Departments of Internal 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