{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T14:49:53Z","timestamp":1785336593702,"version":"3.55.0"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T00:00:00Z","timestamp":1534723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ther Apher Dial"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The accurate estimation of ABO antibody titers is of the utmost importance in organ transplants involving ABO incompatibility. We aim to compare five different methods of titration and analyze the data. Samples of 48 O group blood donors who donated during the month of December 2015 to January 2016 in our institution were subjected to ABO antibody titration by five different methods: immediate spin (IS) tube titer, antihuman globulin phase tube titer, Coomb's gel card titer, gel card titer after dithiotreitol (DTT) treatment of plasma, and the solid phase red cell adherence method. The mean number of titer serial dilution steps in the different titer estimation methods was compared using the paired <jats:italic>t<\/jats:italic>\u2010test and McNemar test. A correlation between the methods was tested using Spearman's rho and kappa statistics. The median antiglobulin (AHG) phase tube titers were found to be the highest anti\u2010A (128) and anti\u2010B (192) titers. Significant differences in the ABO antibody titer readings among the five different methods were noted. Titers were reduced by DTT treatment in nearly 50% samples tested for both anti\u2010A and anti\u2010B titers. Average agreements between the DTT\u2010applied AHG phase gel card titers and the solid phase red cell adherence (SPRCA) titers was observed for anti\u2010A (<jats:italic>\u03ba<\/jats:italic> = 0.473) and anti\u2010B (<jats:italic>\u03ba<\/jats:italic> = 0.530). The AHG phase tube and gel cards titers showed poor agreements. There are differences in the interpretability of the ABO antibody titer among different techniques. Consistent and uniform application of the method for titration throughout the treatment of a patient is highly essential.<\/jats:p>","DOI":"10.1111\/1744-9987.12745","type":"journal-article","created":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T10:51:53Z","timestamp":1534762313000},"page":"86-91","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Comparative Evaluation of Five Different Methods of Anti\u2010ABO Antibody Titration: An Aid for ABO\u2010Incompatible Organ Transplants"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7941-5473","authenticated-orcid":false,"given":"Sweta","family":"Nayak","sequence":"first","affiliation":[{"name":"Transfusion Medicine Indraprastha Apollo Hospital  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raj Nath","family":"Makroo","sequence":"additional","affiliation":[{"name":"Transfusion Medicine Indraprastha Apollo Hospital  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bindu","family":"Prakash","sequence":"additional","affiliation":[{"name":"Transfusion Medicine Indraprastha Apollo Hospital  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trilok","family":"Chandra","sequence":"additional","affiliation":[{"name":"Transfusion Medicine Indraprastha Apollo Hospital  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soma","family":"Agrawal","sequence":"additional","affiliation":[{"name":"Transfusion Medicine Indraprastha Apollo Hospital  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohit","family":"Chowdhry","sequence":"additional","affiliation":[{"name":"Transfusion Medicine Indraprastha Apollo Hospital  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archisman","family":"Mohapatra","sequence":"additional","affiliation":[{"name":"Transfusion Medicine Indraprastha Apollo Hospital  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,20]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"337","volume-title":"Technical Manual","author":"Storry JR","year":"2014"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1309\/LM1O8F7MLLYIPIMZ"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3343\/lmo.2011.1.1.9"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2014.34.4.300"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03049.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3089.2006.00296.x"},{"issue":"2","key":"e_1_2_6_8_1","first-page":"177","article-title":"Platelet antibody screening by flow cytometry is more sensitive than solid phase red cell adherence assay and lymphocytotoxicity technique: a comparative study in Thai patients","volume":"28","author":"Buakaew J","year":"2010","journal-title":"Asian Pac J Allergy Immunol"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-6247.137442"},{"key":"e_1_2_6_10_1","unstructured":"The Drug and Cosmetics Act 1940 and the Drug and Cosmetics Rules 1945 as amended up to 30th June 2005. Schedule F. Part XIIB. Central Drugs Standard Control Organization. Director General of Health Services. Ministry of Health and Family Welfare. Government of India; 268\u2013288. Available from URL:http:\/\/www.cdsco.nic.in\/writereaddata\/drugs&cosmeticact.pdf"},{"key":"e_1_2_6_11_1","unstructured":"Standards For Blood Banks & Blood Transfusion Services National AIDS Control Organization Ministry of Health and Family Welfare Government of India New Delhi 2007"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-4065.202402"},{"issue":"1","key":"e_1_2_6_13_1","first-page":"87","article-title":"ABO\u2010incompatible solid\u2010organ transplantation","volume":"125","author":"Warner PR","year":"2006","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000438210.27218.9e"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13770"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2014.34.6.456"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02478.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2008.01043.x"}],"container-title":["Therapeutic Apheresis and Dialysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1744-9987.12745","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-9987.12745","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T12:34:35Z","timestamp":1694694875000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-9987.12745"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,20]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1111\/1744-9987.12745"],"URL":"https:\/\/doi.org\/10.1111\/1744-9987.12745","archive":["Portico"],"relation":{},"ISSN":["1744-9979","1744-9987"],"issn-type":[{"value":"1744-9979","type":"print"},{"value":"1744-9987","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,20]]},"assertion":[{"value":"2017-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}