{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T22:52:07Z","timestamp":1787611927541,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,8,13]],"date-time":"2022-08-13T00:00:00Z","timestamp":1660348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2020JJ4405"],"award-info":[{"award-number":["2020JJ4405"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ther Apher Dial"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To elucidate the mechanism of miR\u2010128\u20103p in peritoneal fibrosis (PF).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peritoneal mesothelial cells (PMCs) were dealt with high glucose (HG) for 3\u2009days. The expressions of miR\u2010128\u20103p, p21\u2010activated kinase 2 (PAK2), spleen tyrosine kinase (SyK), and transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21) were detected with quantitative real\u2010time reverse transcription polymerase chain reaction. The levels of IL\u20101\u03b2, TNF\u2010\u03b1, IL\u20106, and monocyte chemotactic protein\u20101 in supernatant were measured by ELISA. Proteins of TGF\u2010\u03b21, SyK, PAK2, \u03b1\u2010SMA, collagen I, vimentin, ERK\/AP\u20101, and I\u03baB\u03b1\/NF\u2010\u03baB pathway related proteins were measured by Western blot. The correlation between miR\u2010128\u20103p and PAK2 was found by bioinformatics analysis and luciferase reporter gene analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>miR\u2010128\u20103p was decreased while PAK2, SyK, and TGF\u2010\u03b21 were increased in HG\u2010induced PMCs. Moreover, miR\u2010128\u20103p inhibited HG\u2010induced fibrosis and inflammation in PMCs by targeting PAK2. PAK2 activated SyK, which induced TGF\u2010\u03b21 expression through ERK\/AP\u20101 and I\u03baB\u03b1\/NF\u2010\u03baB pathways to promote HG\u2010induced fibrosis of PMCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>miR\u2010128\u20103p inhibited HG\u2010induced PMCs fibrosis via PAK2\/SyK\/TGF\u2010\u03b21 axis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1744-9987.13912","type":"journal-article","created":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T12:28:40Z","timestamp":1659011320000},"page":"343-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>miR<\/scp>\u2010128\u20103p inhibits high\u2010glucose\u2010induced peritoneal mesothelial cells fibrosis via <scp>PAK2<\/scp>\/<scp>SyK<\/scp>\/<scp>TGF<\/scp>\u2010\u03b21 axis"],"prefix":"10.1111","volume":"27","author":[{"given":"Yao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Nephrology and Laboratory of Kidney Disease Hunan Provincial People's Hospital, First Affiliated Hospital of Hunan Normal University, Changsha Clinical Research Center for Kidney Disease, Hunan Clinical Research Center for Chronic Kidney Disease  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu\u2010Hao","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Laboratory of Kidney Disease Hunan Provincial People's Hospital, First Affiliated Hospital of Hunan Normal University, Changsha Clinical Research Center for Kidney Disease, Hunan Clinical Research Center for Chronic Kidney Disease  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Xiong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Laboratory of Kidney Disease Hunan Provincial People's Hospital, First Affiliated Hospital of Hunan Normal University, Changsha Clinical Research 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