{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T21:20:54Z","timestamp":1793049654582,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T00:00:00Z","timestamp":1748131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010957","name":"Hamamatsu Photonics K.K.","doi-asserted-by":"publisher","award":["FLP\u2010H4200b"],"award-info":[{"award-number":["FLP\u2010H4200b"]}],"id":[{"id":"10.13039\/501100010957","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ther Apher Dial"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Activated neutrophils play a crucial role in the development of neutrophilic dermatoses, including pustular psoriasis. Adsorptive granulocyte and monocyte apheresis (GMA) is an effective treatment for skin disorders. Although previous basic research has demonstrated neutrophil activation, no efficient method exists to assess it in clinical settings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We measured neutrophil activity in patients with GPP who underwent GMA therapy using the FLP\u2010H4200, a newly developed neutrophil activity evaluation system. Blood samples were collected before the first and after the fifth GMA session.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In Case 1, which responded to GMA, the neutrophil activation value decreased from 118\u2009603 to 26\u2009234, reaching the normal range. In Case 2, the response to GMA was poor, and the neutrophil activation value decreased from 140\u2009623 to 76\u2009780, which remained significantly above the normal level.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that the neutrophil activity evaluation system may be useful for assessing disease severity and therapeutic efficacy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1744-9987.70043","type":"journal-article","created":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T04:40:19Z","timestamp":1748234419000},"page":"702-706","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of Neutrophil Activity Before and After Granulocyte and Monocyte Adsorptive Apheresis in Patients With Pustular Psoriasis"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3867-0555","authenticated-orcid":false,"given":"Yuko","family":"Higashi","sequence":"first","affiliation":[{"name":"Department of Dermatology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"},{"name":"Kagoshima City Hospital  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2349-1765","authenticated-orcid":false,"given":"Atsuko","family":"Ibusuki","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Fukushige","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoko","family":"Sagara","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Risako","family":"Yonezawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natsumi","family":"Terada","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisao","family":"Kawahira","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8628-5785","authenticated-orcid":false,"given":"Kimiko","family":"Kazumura","sequence":"additional","affiliation":[{"name":"Global Strategic Challenge Center Hamamatsu Photonics K.K.  Shizuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3904-5954","authenticated-orcid":false,"given":"Takuro","family":"Kanekura","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,25]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"457","volume-title":"Fitzpatrick's Dermatology","author":"Kang S.","year":"2019"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.14523"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2012.09.037"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.631821"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232012137"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42477-y"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbsr.2024.100722"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201093887\u20101"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.24-61"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.22\u2010109"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.23-50"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0200573"},{"key":"e_1_2_7_14_1","unstructured":"K.Kazumura M.Hattori andY.Hatano \u201cCalculation Method Calculation Device and Calculation Program \u201d US Patent Application Publication 0168483 Al 2023."},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060815-014442"},{"issue":"1297","key":"e_1_2_7_16_1","article-title":"Genetics of Generalized Pustular Psoriasis: Current Understanding and Implications for Future Therapeutics","volume":"14","author":"Yang S. F.","year":"2023","journal-title":"Genes (Basel)"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2020.06.020"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.16700"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.14471"}],"container-title":["Therapeutic Apheresis and Dialysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1744-9987.70043","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T02:12:10Z","timestamp":1751422330000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1744-9987.70043"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,25]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/1744-9987.70043"],"URL":"https:\/\/doi.org\/10.1111\/1744-9987.70043","archive":["Portico"],"relation":{},"ISSN":["1744-9979","1744-9987"],"issn-type":[{"value":"1744-9979","type":"print"},{"value":"1744-9987","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,25]]},"assertion":[{"value":"2024-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}