{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:43:48Z","timestamp":1792028628995,"version":"4.4.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T00:00:00Z","timestamp":1627603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2021M690577"],"award-info":[{"award-number":["2021M690577"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Integr. Zool."],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Identifying the mechanism for sex determination in amphibians is challenging. Very little is known about sex determination mechanisms of <jats:italic>Rana dybowskii<\/jats:italic>, a species of importance to evolutionary and conservation biology. We screened for sex\u2010linked molecular markers in <jats:italic>R. dybowskii<\/jats:italic> in China using target region amplification polymorphism with 2 fixed primers against the sequences of <jats:italic>Dmrt1<\/jats:italic>. We found 2 male\u2010linked molecular markers in <jats:italic>R. dybowskii<\/jats:italic>, which were 222 bp and 261 bp long. The detection rates of 222 bp marker in males form Xinglong, Huadian, and Dandong were 93.79%, 69.64%, and 13.64%, respectively, while the rate in females from Huadian was 27.50%. Besides, the detection rates of 261 bp marker in the above 3 regions were only observed in males at the rate of 93.79%, 87.50%, and 32.73%, respectively. The inheritance patterns of sex\u2010linked molecular markers showed that the 2 sex\u2010linked molecular markers were heterozygous. Compared to the XY\u2010male parent, progeny from XX\u2010pseudo\u2010male parent possessed lower sex reversal ratio at the same rearing temperature, and the proportion of female froglets from an XX\u2010pseudo\u2010male parent was more than 95% at low rearing temperature (15\u00b0C). Our findings suggest that <jats:italic>R. dybowskii<\/jats:italic> displays male heterogamety, and the 2 sex\u2010linked molecular markers may have a guiding significance for the protection and utilization of <jats:italic>R. dybowskii<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/1749-4877.12577","type":"journal-article","created":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T06:53:35Z","timestamp":1626159215000},"page":"105-114","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Male heterogametic sex determination in <i>Rana dybowskii<\/i> based on sex\u2010linked molecular markers"],"prefix":"10.1111","volume":"17","author":[{"given":"Yuan","family":"XU","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Northeast Agricultural University  Harbin China"},{"name":"College of Life Sciences Northeast Agricultural University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiheng","family":"DU","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northeast Agricultural University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"LIU","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northeast Agricultural University  Harbin China"},{"name":"Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences  Qiqihar China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"SU","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northeast Agricultural University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangyong","family":"NING","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northeast Agricultural University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiquan","family":"CUI","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northeast Agricultural University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"WANG","sequence":"additional","affiliation":[{"name":"College of Agricultural Eastern Liaoning University  Dandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"LIU","sequence":"additional","affiliation":[{"name":"Yili Animal Science Research Institute  Yining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanshuai","family":"REN","sequence":"additional","affiliation":[{"name":"Animal Husbandry Administration of Huadian  Huadian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengwei","family":"DI","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northeast Agricultural University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiujuan","family":"BAI","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northeast Agricultural University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2010.04607.x"},{"key":"e_1_2_7_3_1","unstructured":"Amphibia Web(2007).Rana dybowskii: Dybovsky's frog. 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