{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T07:12:36Z","timestamp":1772694756578,"version":"3.50.1"},"reference-count":116,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T00:00:00Z","timestamp":1637884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Integr. Zool."],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The antioxidant defense system is essential for animals to cope with homeostasis disruption and overcome oxidative stress caused by adverse environmental conditions such as hypoxia. However, our understanding of how this system works in subterranean rodents remains limited. In this study, Brandt's vole <jats:italic>Lasiopodomys brandtii<\/jats:italic> was exposed to normoxia (21% O<jats:sub>2<\/jats:sub>) or hypoxia (mild or severe hypoxia: 10% or 5% O<jats:sub>2<\/jats:sub>) for 6 h. Changes in key enzymes of the classic enzymatic antioxidant system at both mRNA and enzyme activity levels, and tissue antioxidant levels of the low\u2010molecular\u2010weight antioxidant system were determined in brain, liver, and kidney. Transcript levels of the upstream regulator NF\u2010E2\u2010related factor 2 (<jats:italic>Nrf2<\/jats:italic>) were also measured. We found that the mRNA expression of <jats:italic>Nrf2<\/jats:italic> and its downstream antioxidant enzyme genes in <jats:italic>L. brandtii<\/jats:italic> were relatively conserved in response to hypoxia in most tissues and genes tested, except in the liver. Hepatic <jats:italic>Nrf2<\/jats:italic>, <jats:italic>Cu\/Zn SOD<\/jats:italic>, <jats:italic>GPx1<\/jats:italic>, and <jats:italic>GPx3<\/jats:italic> levels were significantly upregulated in response to mild hypoxia, whereas <jats:italic>Mn SOD<\/jats:italic> level decreased significantly in severe hypoxia. Unmatched with changes at the RNA level, constitutively high and relatively stable antioxidant enzyme activities were maintained throughout. For the low\u2010molecular\u2010weight antioxidant system, an abrupt increase of cerebral ascorbic acid (AA) levels in hypoxia indicated a tissue\u2010specific antioxidant response. Although hypoxia did not cause significant oxidative damage in most tissues tested, the significant decrease in antioxidant enzyme activities (GPX and GR) and increase in lipid peroxidation in the kidney suggest that prolonged hypoxia may pose a critical threat to this species.<\/jats:p>","DOI":"10.1111\/1749-4877.12602","type":"journal-article","created":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T08:04:00Z","timestamp":1635494640000},"page":"581-595","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Antioxidant response to severe hypoxia in Brandt's vole <i>Lasiopodomys brandtii<\/i>"],"prefix":"10.1111","volume":"17","author":[{"given":"Luye","family":"SHI","sequence":"first","affiliation":[{"name":"School of Life Sciences Zhengzhou University  Zhengzhou China"},{"name":"State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bojian","family":"CHEN","sequence":"additional","affiliation":[{"name":"College of Environmental Science and Engineering Tongji University  Shanghai China"},{"name":"Jiaxing\u2010Tongji Environmental Research Institute  Jiaxing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinrui","family":"WANG","sequence":"additional","affiliation":[{"name":"School of Life Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maolin","family":"HUANG","sequence":"additional","affiliation":[{"name":"School of Life Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congcong","family":"QIAO","sequence":"additional","affiliation":[{"name":"School of Life Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingou","family":"WANG","sequence":"additional","affiliation":[{"name":"School of Life Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenlong","family":"WANG","sequence":"additional","affiliation":[{"name":"School of Life Sciences Zhengzhou University  Zhengzhou China"},{"name":"Centre for Nutritional Ecology Zhengzhou University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00584-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-3414fje"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2018.07.001"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmammal\/gyw104"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/WOX.0b013e3182439613"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2012.11.020"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00359-006-0111-z"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S275595"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00343-019-7345-4"},{"key":"e_1_2_8_11_1","first-page":"4","article-title":"Antioxidant response to acute cold exposure and following recovery in juvenile Chinese soft\u2010shelled turtles, Pelodiscus sinensis","volume":"222","author":"Chen B","year":"2019","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2017.02.014"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-0981(02)00329-5"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2020.113904"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11756-020-00469-z"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2012.09.018"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709472105"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-018-5318-1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12983-020-00356-y"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01969-1"},{"key":"e_1_2_8_21_1","volume-title":"Choosing and Using Statistics","author":"Dytham","year":"2011"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdr2.1142"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410320706"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.4705"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00484-020-02037-1"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00831.2013"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2020.05.018"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1532-0456(02)00080-7"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/aje.12448"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.17582\/journal.pjz\/2019.51.4.1519.1526"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2010.12.009"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9493"},{"key":"e_1_2_8_33_1","first-page":"124","article-title":"Reactive oxygen species \u2010 sources, functions, oxidative damage","volume":"48","author":"Jakubczyk K","year":"2020","journal-title":"Polski Merkuriusz Lekarski"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12237-016-0169-y"},{"key":"e_1_2_8_35_1","first-page":"179","article-title":"Study on the habitat characteristics of Brandt's vole","volume":"20","author":"Jiang Y","year":"2012","journal-title":"Acta Agrestia Sinica"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(02)48630-2"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.46.120604.141046"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200318182"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph16132320"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2009.10.005"},{"key":"e_1_2_8_41_1","first-page":"559","article-title":"Life Underground: The biology of subterranean rodents","volume":"107","author":"Lacey E","year":"2010","journal-title":"Ethology"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0b013e32833370cf"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.206896"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00010.2014"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-013-9835-1"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862-017-0995-y"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-020-01117-w"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.20465"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.15012"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22658"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.215905"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1890\/13-1499.1"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2010.10.004"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2006.02.014"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2007.07.007"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2005.01.006"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2018.03.290"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0952836901000346"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-615"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11160-005-7846-4"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403061200"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(84)90353-8"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2011.05.004"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.27.956"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2018.10.013"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2012.09.008"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R900010200"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(02)00132-0"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90738-3"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-445X(01)00226-0"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2007.11.006"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(02)01137-1"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(01)00284-3"},{"key":"e_1_2_8_74_1","first-page":"534","article-title":"Effects of transportation on antioxidant status in cynomolgus macaques (Macaca fascicularis)","volume":"55","author":"Pan X","year":"2016","journal-title":"Journal of the American Association for Laboratory Animal Science"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab3896"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2011.11.042"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1139\/a06-006"},{"key":"e_1_2_8_78_1","first-page":"127","article-title":"The impact of voles density on plant community structure and root system","volume":"26","author":"Qin J","year":"2009","journal-title":"Pratacultural Science"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.116112"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03193172"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.11.038"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-13905-z"},{"key":"e_1_2_8_83_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_8_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2012.03.019"},{"key":"e_1_2_8_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2018.03.001"},{"key":"e_1_2_8_86_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-010-0195-y"},{"key":"e_1_2_8_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2005.09.003"},{"key":"e_1_2_8_88_1","doi-asserted-by":"publisher","DOI":"10.1139\/Z10-049"},{"key":"e_1_2_8_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/1043-6618(95)86904-2"},{"key":"e_1_2_8_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2018.06.013"},{"key":"e_1_2_8_91_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-008-9145-7"},{"key":"e_1_2_8_92_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0240435"},{"key":"e_1_2_8_93_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1427.015"},{"key":"e_1_2_8_94_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2006.03.019"},{"key":"e_1_2_8_95_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00563"},{"key":"e_1_2_8_96_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtherbio.2018.11.003"},{"key":"e_1_2_8_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2020.125819"},{"key":"e_1_2_8_98_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2021.01.015"},{"key":"e_1_2_8_99_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)40252-4"},{"key":"e_1_2_8_100_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2016.0135"},{"key":"e_1_2_8_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2006.07.001"},{"key":"e_1_2_8_102_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803833105"},{"key":"e_1_2_8_103_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S142008"},{"key":"e_1_2_8_104_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.735903"},{"key":"e_1_2_8_105_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201708007"},{"key":"e_1_2_8_106_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.180068"},{"key":"e_1_2_8_107_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.5.2062"},{"key":"e_1_2_8_108_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006817806010"},{"key":"e_1_2_8_109_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-019-2726-y"},{"key":"e_1_2_8_110_1","first-page":"1253","article-title":"Change trend and regional differentiation of precipitation over the Mongolian Plateau in recent 54 years","volume":"06","author":"Yin\u2010Tai N","year":"2019","journal-title":"Arid Land Geography"},{"key":"e_1_2_8_111_1","first-page":"51","article-title":"On the colonial structure of Brandt's vole in burrow units","volume":"1","author":"Zhang J","year":"1981","journal-title":"Acta Theriologica Sinica"},{"key":"e_1_2_8_112_1","doi-asserted-by":"publisher","DOI":"10.5194\/bg-7-1443-2010"},{"key":"e_1_2_8_113_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2011.07.026"},{"key":"e_1_2_8_114_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2009.08.018"},{"key":"e_1_2_8_115_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-019-01206-y"},{"key":"e_1_2_8_116_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00710"},{"key":"e_1_2_8_117_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2016.10.014"}],"container-title":["Integrative Zoology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1749-4877.12602","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1749-4877.12602","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1749-4877.12602","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T09:07:38Z","timestamp":1692781658000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1749-4877.12602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,26]]},"references-count":116,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1111\/1749-4877.12602"],"URL":"https:\/\/doi.org\/10.1111\/1749-4877.12602","archive":["Portico"],"relation":{},"ISSN":["1749-4877","1749-4877"],"issn-type":[{"value":"1749-4877","type":"print"},{"value":"1749-4877","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,26]]},"assertion":[{"value":"2021-11-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}