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Zool."],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new species of <jats:italic>Dugesia<\/jats:italic> (Platyhelminthes, Tricladida, Dugesiidae) from northern China is described on the basis of an integrative approach, involving morphology, karyology, histology, molecular distance, molecular phylogeny, and mitochondrial gene order. Here, we present the complete mitogenome of the new species <jats:italic>Dugesia constrictiva<\/jats:italic> Chen &amp; Dong, <jats:bold>sp. nov<\/jats:bold>. This new species is mainly characterized by the presence of the following features: asymmetrical openings of the oviducts; large, cuboidal copulatory bursa; vasa deferentia opening through the ventro\u2010lateral wall of the seminal vesicle; laterally compressed seminal vesicle; ventrally displaced ejaculatory duct, opening at the blunt tip of the penis papilla; long duct intercalated between seminal vesicle and diaphragm; chromosome complement diploid, with 16 metacentric chromosomes; mitochondrial gene order as follows: <jats:italic>cox1\u2010E\u2010nad6\u2010nad5\u2010S2\u2010D\u2010R\u2010cox3\u2010I\u2010Q\u2010K\u2010atp6\u2010V\u2010nad1\u2010W\u2010cox2\u2010P\u2010nad3\u2010A\u2010nad2\u2010M\u2010H\u2010F\u2010rrnS\u2010L1\u2010Y\u2010G\u2010S1\u2010rrnL\u2010L2\u2010T\u2010atp8\u2010C\u2010N\u2010cob\u2010nad4l\u2010nad4<\/jats:italic>. In triclads, mitochondrial gene order is considerably conserved between freshwater planarians and land flatworms, whereas it is variable between marine planarians and both freshwater and land flatworms. The secondary structures of tRNAs are all equipped with 4 arms, excepting <jats:italic>tRNA S1<\/jats:italic> and <jats:italic>tRNA S2<\/jats:italic>, which lack the D arm and have excessively enlarged loops. Numerous transpositions of tRNA are present between <jats:italic>D. constrictiva<\/jats:italic> and its congeners. Mitochondrial gene arrangements may form a new, additional tool for taxonomic studies. The phylogenetic tree based on analysis of the mitochondrial genome basically corroborates current classification of the higher taxa of planarian flatworms.<\/jats:p>","DOI":"10.1111\/1749-4877.12605","type":"journal-article","created":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T07:52:25Z","timestamp":1637049145000},"page":"1193-1214","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Integrative taxonomy unveils a new species of <i>Dugesia<\/i> (Platyhelminthes, Tricladida, Dugesiidae) from the southern portion of the Taihang Mountains in northern China, with the description of its complete mitogenome and an exploratory analysis of mitochondrial gene order as a taxonomic character"],"prefix":"10.1111","volume":"17","author":[{"given":"Lei","family":"WANG","sequence":"first","affiliation":[{"name":"College of Life Science Henan Normal University  Xinxiang China"},{"name":"Medical College, Xinxiang University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"WANG","sequence":"additional","affiliation":[{"name":"College of Life Science Henan Normal University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zimei","family":"DONG","sequence":"additional","affiliation":[{"name":"College of Life Science Henan Normal University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangwen","family":"CHEN","sequence":"additional","affiliation":[{"name":"College of Life Science Henan Normal University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"SLUYS","sequence":"additional","affiliation":[{"name":"Naturalis Biodiversity Center  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dezeng","family":"LIU","sequence":"additional","affiliation":[{"name":"College of Life Science Henan Normal University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq291"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2008.01.032"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1163\/26660644-04802007"},{"key":"e_1_2_7_5_1","first-page":"237","article-title":"Cytotaxonomical study of Dugesia indonesiana Kawakatsu (Tricladida Paludicola)","volume":"58","author":"Benazzi M","year":"1975","journal-title":"Atti della Accademia Nazionale dei Lincei. 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