{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T02:24:09Z","timestamp":1789611849641,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T00:00:00Z","timestamp":1647475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Integr. Zool."],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Potential zoonotic pathogens may be transmitted from wildlife to humans through the illegal wild meat trade, which has become a pressing issue. However, research on the antimicrobial resistance genes (ARGs) of Malayan pangolin\n                    <jats:italic>(Manis javanica)<\/jats:italic>\n                    intestinal bacteria is limited. Here, multidrug\u2010resistant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    M172\u20101 (ST354) isolated from Malayan pangolin feces in 2019 was found to be resistant to 13 antibiotics. BGWAS analysis revealed 4 plasmids, namely, pM172\u20101.1, pM172\u20101.2, pM172\u20101.3, and pM172\u20101.4, in the isolate. The pM172\u20101.2, pM172\u20101.3, and pM172\u20101.4 plasmids carried ARGs, namely, IncHI2\u2010HI2A, IncX1\u2010X1, and IncX1, respectively. pM172\u20101.3 and pM172\u20101.4 contained intact IntI1 integrons (\n                    <jats:italic>Is26\/IntI1\/arr2\/cmlA5\/bla<\/jats:italic>\n                    <jats:sub>OXA\u201010<\/jats:sub>\n                    <jats:italic>\/ant(3\u2033)\u2010IIA\/dfrA14\/Is26<\/jats:italic>\n                    ). Notably, pM172\u20101.3 resulted from the fusion of 2 pM172\u20101.4 copies and carried many more ARGs. In addition to pM172\u20101.3 from the same host, other drug\u2010resistant bacteria (\n                    <jats:italic>E. coli<\/jats:italic>\n                    M159\u20101 (ST48),\n                    <jats:italic>E. coli<\/jats:italic>\n                    S171\u20101 (ST206), and\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    S174\u20101 (ST2354)) in the same\n                    <jats:italic>Malayan pangolin<\/jats:italic>\n                    fecal samples also carried 3 plasmids with 100% gene coverage of pM172\u20101.4 and 99.98% identity. Therefore, ARGs in IncX1 might spread in the intestinal flora of Malayan pangolin and between species via the illegal food chain, posing a potential threat to public health and safety.\n                  <\/jats:p>","DOI":"10.1111\/1749-4877.12637","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T13:48:20Z","timestamp":1645537700000},"page":"289-298","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Characteristics of the multiple replicon plasmid IncX1\u2010X1 in multidrug\u2010resistant\n                    <i>Escherichia coli<\/i>\n                    \u00a0from Malayan pangolin (\n                    <i>Manis javanica<\/i>\n                    )"],"prefix":"10.1111","volume":"18","author":[{"given":"Fang","family":"JI","sequence":"first","affiliation":[{"name":"Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology Guangdong Academy of Science  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelan","family":"LIU","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Zhejiang Center of Disease Control and Prevention  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"WANG","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology Guangdong Academy of Science  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianan","family":"ZHAO","sequence":"additional","affiliation":[{"name":"Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology 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