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RT\u2010qPCR analysis showed that mitophagy\u2010promoting genes, such as <jats:italic>Pink1<\/jats:italic>, <jats:italic>Prkn<\/jats:italic>, <jats:italic>Tomm7<\/jats:italic>, <jats:italic>Mnf2<\/jats:italic>, <jats:italic>Lc3<\/jats:italic>, <jats:italic>Optn<\/jats:italic>, <jats:italic>Gabarap<\/jats:italic>, and <jats:italic>Nbr1<\/jats:italic> were all upregulated in LP. Fluorescence <jats:italic>in situ<\/jats:italic> hybridization indicated that <jats:italic>Pink1<\/jats:italic> expression was present in spermatogonia in SP, while in LP, <jats:italic>Pink1<\/jats:italic> expression extended to almost all germ cell types with significantly higher mean optical density. <jats:italic>Prkn<\/jats:italic> expression was found in all germ cell types in both LP and SP, with a significantly higher mean optical density of 10\u2010week\u2010old LP males. Transmission electron microscopy showed normal mitochondrial morphology with clear membranes in SP, while the LP group had reduced cristae in mitochondria and damaged mitochondria undergoing autophagy. This study suggests that mitophagy may be involved in the photoperiodic spermatogenesis in Brandt's voles, providing insights into the role of photoperiod in seasonal reproduction in wild animals.<\/jats:p>","DOI":"10.1111\/1749-4877.12818","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T03:29:13Z","timestamp":1711942153000},"page":"1105-1120","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Photoperiod\u2010regulated mitophagy in the germ cells of Brandt's voles (<i>Lasiopodomys brandtii<\/i>)"],"prefix":"10.1111","volume":"19","author":[{"given":"Lijuan","family":"ZHAO","sequence":"first","affiliation":[{"name":"School of Life Sciences Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxiao","family":"CHEN","sequence":"additional","affiliation":[{"name":"School of Life Sciences Zhengzhou University  Zhengzhou Henan 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