{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T21:09:11Z","timestamp":1766869751573,"version":"3.44.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,12,8]],"date-time":"2024-12-08T00:00:00Z","timestamp":1733616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Integr. Zool."],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Desmans belong to the subfamily Desmaninae, which are members of the family Talpidae. Desmans and moles show limited sexual dimorphism, making unclear sex discrimination by phenotypic assessment. The Iberian desman (<jats:italic>Galemys pyrenaicus<\/jats:italic>) is an endangered species with a severe population decline. Knowledge of sex and sex ratio is essential for conservation and management. Based on these arguments and although previous conventional PCR studies amplifying DBX\/DBY genes were relatively successful in sexing the desman, high\u2010resolution sex\u2010specific PCR has been requested. All these reasons encouraged us to develop new species\u2010specific RT\u2010qPCR assays by TaqMan probes to determine the sex in desman, especially with genetic material from non\u2010invasive samples. Accordingly, efficiency, limit of detection (LOD), specificity, and DNA analysis from faeces were verified. The target genes DBX and DBY were amplified with gDNA from both sexes, with Y\u2010chromosome consistently absent in the female. Despite the modest efficiency, regression analysis (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> &gt; 0.999) indicated a linear range of the DBX and DBY assays extending from 20 to 0.2 ng\/\u00b5L DNA. LOD analyses estimated that twice as much gDNA was needed in males as in females for DBX detection. Paradoxically, the Y\u2010chromosome required three times as much gDNA as the X\u2010chromosome using a male sample. Therefore, an unexpected dosage imbalance in the genome in favour of the X chromosome was discussed in light of an apparent multicopy nature of the DBX gene and with a sexing success rate of 49.9% of the non\u2010invasive samples, supporting Fisher's principle for the mammalian XX\/XY sex system, as expected.<\/jats:p>","DOI":"10.1111\/1749-4877.12933","type":"journal-article","created":{"date-parts":[[2024,12,8]],"date-time":"2024-12-08T21:29:46Z","timestamp":1733693386000},"page":"1047-1055","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Method Based on Hydrolysis Probe Assays to Identify the Sex Chromosomes of Iberian Desman (<i>Galemys pyrenaicus<\/i>) Using Non\u2010Invasive Sampling"],"prefix":"10.1111","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7786-1173","authenticated-orcid":false,"given":"Adriana","family":"Ripa","sequence":"first","affiliation":[{"name":"Genetic and Animal Breeding, Faculty of Veterinary Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9924-2290","authenticated-orcid":false,"given":"Mar\u00eda Jes\u00fas","family":"Palacios\u2010Gonzalez","sequence":"additional","affiliation":[{"name":"Direcci\u00f3n General de Sostenibilidad Junta de Extremadura  M\u00e9rida Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8866-5626","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"D\u00edaz\u2010Caballero","sequence":"additional","affiliation":[{"name":"Direcci\u00f3n General de Sostenibilidad Junta de Extremadura  M\u00e9rida Spain"},{"name":"\u00c1rea del Medio Natural, Sociedad de Gesti\u00f3n P\u00fablica de Extremadura (GPEX) Junta de Extremadura  M\u00e9rida Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4659-9149","authenticated-orcid":false,"given":"Antonio","family":"Espinosa","sequence":"additional","affiliation":[{"name":"Genetic and Animal Breeding, Faculty of Veterinary Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Javier","family":"Zalba","sequence":"additional","affiliation":[{"name":"\u00c1rea del Medio Natural, Sociedad de Gesti\u00f3n P\u00fablica de Extremadura (GPEX) Junta de Extremadura  M\u00e9rida Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1419-1672","authenticated-orcid":false,"given":"Juan Luis","family":"Garc\u00eda\u2010Zapata","sequence":"additional","affiliation":[{"name":"Department of Mathematics Universidad de Extremadura  Badajoz Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4328-7772","authenticated-orcid":false,"given":"Jos\u00e9 Luis","family":"Fern\u00e1dez\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Genetic and Animal Breeding, Faculty of Veterinary Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748\u20107692.2001.tb01011"},{"volume-title":"Conservation and the Genetics of Populations","year":"2012","author":"Allendorf F. W.","key":"e_1_2_10_3_1"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2010.04.030"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2003.11.025"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2018.03.022"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0250169"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2017.60"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/zoo.21417"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anbehav.2012.03.010"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1055-7903(03)00119-2"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986706776872943"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03192502"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1163\/002829669X00107"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(92)90307-5"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920207780368141"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.16.7276"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-animal-050622-043424"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2005.12.007"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199612)45:4<491::AID-MRD12>3.0.CO;2-#"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-7692.2011.00543.x"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2008.02324.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2005.02651.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.1998.tb00179.x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10592-009-9947-4"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.12141"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10592-007-9371-6"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.2307\/3503939"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1515\/mamm.1956.20.4.405"},{"key":"e_1_2_10_31_1","first-page":"118","article-title":"Dimorphisme Sexuel De la Ceinture Pelvienne D'un Mammifere Insectivore","volume":"244","author":"Peyre A.","year":"1957","journal-title":"Galemys Pyrenaicus G C R Hebd Seances Academy of Sciences"},{"key":"e_1_2_10_32_1","first-page":"434","article-title":"La Formule Chromosomique Du Desman Des Pyrenees Galemys pyrenaicus","volume":"82","author":"Peyre A.","year":"1957","journal-title":"K Bulletin de la Soci\u00e9t\u00e9 Zoologique de France"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaz2582"},{"key":"e_1_2_10_34_1","first-page":"467","article-title":"Le carrefour Tracheen Dans L'adaptation Du Desman Des Pyrenees (Galemys pyrenaicus) a la Vie Dulcaquicole","volume":"39","author":"Richard P. B.","year":"1986","journal-title":"Mammalia"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13071136"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-023-09010-2"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10592-005-9105-6"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp045"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10344-012-0683-6"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/jeb.12415"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10592-009-9913-1"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.2193\/0022-541X(2005)69[1419:NGSTFW]2.0.CO;2"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.5122\/cbirds.2009.0009"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1086\/657918"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(04)01044\u20108"}],"container-title":["Integrative Zoology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1749-4877.12933","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T02:25:51Z","timestamp":1758853551000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1749-4877.12933"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,8]]},"references-count":44,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/1749-4877.12933"],"URL":"https:\/\/doi.org\/10.1111\/1749-4877.12933","archive":["Portico"],"relation":{},"ISSN":["1749-4877","1749-4877"],"issn-type":[{"type":"print","value":"1749-4877"},{"type":"electronic","value":"1749-4877"}],"subject":[],"published":{"date-parts":[[2024,12,8]]},"assertion":[{"value":"2024-05-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}