{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T17:04:54Z","timestamp":1789405494596,"version":"build-2803163510"},"reference-count":73,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Integr. Zool."],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Amphibians host a diverse array of macro and microparasites, and these relationships can provide relevant information for assessing and monitoring population and ecosystem health. The dynamics and outcomes of these host\u2013parasite interactions are influenced by several biotic and abiotic factors, as well as by the characteristics of both the parasite and the host. This study aims to identify hemoparasites in\n                    <jats:italic>Pleurodema thaul<\/jats:italic>\n                    (four\u2010eyed frog) (Leptodactylidae) across its extensive distribution in Chile and to explore potential relationships between parasite occurrence, host traits, and habitat variables. To achieve this, we surveyed\n                    <jats:italic>P. thaul<\/jats:italic>\n                    populations from 40 localities between the extreme north and south of Chile (22\u201343\u00b0S), in a latitudinal gradient of 2500 km. In each locality, individuals were actively searched along the margin of water bodies, manually captured, sexed, and measured using standard morphometric and biosecurity protocols. Blood samples were collected via facial vein puncture using needles and heparinized capillary tubes. Smears were prepared on\u2010site, fixed with methanol, and stained with 10% Giemsa for subsequent microscopic analysis at 40\u00d7 and 100\u00d7 magnification. Hemoparasites were detected in 61% (221\/363) of individuals, with three genera identified: the hemogregarines\n                    <jats:italic>Hepatozoon<\/jats:italic>\n                    sp. and\n                    <jats:italic>Dactylosoma<\/jats:italic>\n                    sp. (Adeleorina), with a prevalence of 7% and 39%, respectively, and the hemococcidium\n                    <jats:italic>Lankesterella<\/jats:italic>\n                    sp. (Eimeriorina), with 22%. The study describes the key characteristics of these parasites in both intra\u2010 and extracellular developmental stages. These findings contribute to the understanding of anuran hemoparasites by expanding knowledge on their distribution, morphological traits, and local and regional host\u2013parasite interactions.\n                  <\/jats:p>","DOI":"10.1111\/1749-4877.70055","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T15:11:00Z","timestamp":1768230660000},"page":"1105-1117","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hemoparasites of the Four\u2010Eyed Frog (\n                    <i>Pleurodema thaul<\/i>\n                    ) From Chile"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4208-7577","authenticated-orcid":false,"given":"Roc\u00edo","family":"Sanhueza\u2010Caba","sequence":"first","affiliation":[{"name":"PhD Program in Conservation Medicine Universidad Andres Bello  Santiago Chile"},{"name":"One Health Institute Universidad Andres Bello  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2077-4666","authenticated-orcid":false,"given":"Daniela","family":"Doussang","sequence":"additional","affiliation":[{"name":"PhD Program in Conservation Medicine Universidad Andres Bello  Santiago Chile"},{"name":"Veterinary Medicine School Universidad Andres Bello  Vi\u00f1a del Mar Chile"},{"name":"One Health Institute Universidad Andres Bello  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9201-7886","authenticated-orcid":false,"given":"Claudio","family":"Azat","sequence":"additional","affiliation":[{"name":"PhD Program in Conservation Medicine Universidad Andres Bello  Santiago Chile"},{"name":"One Health Institute Universidad Andres Bello  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvex.2008.03.006"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11010170"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201096535\u2010w"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010018\u20105822\u2010x"},{"issue":"1","key":"e_1_2_5_6_1","first-page":"190","article-title":"Haematology of Amphibians and Reptiles: A Review","volume":"10","author":"Arikan H.","year":"2014","journal-title":"North\u2010Western Journal of Zoology"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/8259712"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.13610"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2024.105288"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.2307\/3282527"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.15.9031"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","unstructured":"Bilhalva L. 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