{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T19:05:37Z","timestamp":1783969537839,"version":"3.55.0"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007932","name":"Gansu Agricultural University","doi-asserted-by":"publisher","award":["KLGE\u20102024\u201002"],"award-info":[{"award-number":["KLGE\u20102024\u201002"]}],"id":[{"id":"10.13039\/501100007932","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272566"],"award-info":[{"award-number":["32272566"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Integr. Zool."],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sterility control is one of the key tools for regulating pest rodent population density. An in\u2010depth analysis of the molecular mechanism of sterility caused by control agents is of great significance for further exploration of novel sterility controls and the development of alternative drugs. In this study, male plateau zokors (\n                    <jats:italic>Eospalax baileyi<\/jats:italic>\n                    ) in the breeding period were tested to explore the molecular mechanism of quinestrol\u2010induced sterility. We used RNA\u2010seq technology to investigate key genes and signaling pathways associated with the inhibition of testicular development and spermatogenesis, and validated these findings through qPCR. The findings indicated that in plateau zokors treated with quinestrol, 420 genes were down\u2010regulated and 127 genes were up\u2010regulated.\n                    <jats:italic>Notch3<\/jats:italic>\n                    ,\n                    <jats:italic>Ppp2r3c<\/jats:italic>\n                    ,\n                    <jats:italic>Lipe<\/jats:italic>\n                    ,\n                    <jats:italic>Il1b<\/jats:italic>\n                    , and\n                    <jats:italic>Tlr2<\/jats:italic>\n                    are the potential new targets for quinestrol to affect testicular development in plateau zokors. Gene ontology (GO) analysis showed that DEGs were enriched in the inflammatory response, positive regulation of ERK1 and ERK2 cascades, and positive regulation of MAPK cascades. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs were enriched in pathways such as metabolism of xenobiotics by cytochrome P450. GSEA analysis revealed that treatment with quinestrol induced pathway changes related to the positive regulation of the ERK1 and ERK2 cascades and the positive regulation of PI3K\/AKT signaling in plateau zokors. Quinestrol influences the ERK1\/2 signaling pathway within the MAPK cascade in spermatogonia of plateau zokor testes via the GPER1 receptor, inducing oxidative stress and resulting in male infertility.\n                  <\/jats:p>","DOI":"10.1111\/1749-4877.70059","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T10:16:27Z","timestamp":1769595387000},"page":"928-942","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Mechanism of Sterilization by Quinestrol, a Rodent Sterility Control Agent"],"prefix":"10.1111","volume":"21","author":[{"given":"Yanan","family":"Deng","sequence":"first","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem (Ministry of Education), College of Grassland Science Gansu Agricultural University Lanzhou China"},{"name":"Gansu Agricultural University\u2013Massey University Research Centre for Grassland Biodiversity Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukun","family":"Kang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem (Ministry of Education), College of Grassland Science Gansu Agricultural University Lanzhou China"},{"name":"Gansu Agricultural University\u2013Massey University Research Centre for Grassland Biodiversity Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"An","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem (Ministry of Education), College of Grassland Science Gansu Agricultural University Lanzhou China"},{"name":"Gansu Agricultural University\u2013Massey University Research Centre for Grassland Biodiversity Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baohui","family":"Yao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem (Ministry of Education), College of Grassland Science Gansu Agricultural University Lanzhou China"},{"name":"Gansu Agricultural University\u2013Massey University Research Centre for Grassland Biodiversity Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Tan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem (Ministry of Education), College of Grassland Science Gansu Agricultural University Lanzhou China"},{"name":"Gansu Agricultural University\u2013Massey University Research Centre for Grassland Biodiversity Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kejie","family":"Ou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem (Ministry of Education), College of Grassland Science Gansu Agricultural University Lanzhou China"},{"name":"Gansu Agricultural University\u2013Massey University Research Centre for Grassland Biodiversity Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyao","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem (Ministry of Education), College of Grassland Science Gansu Agricultural University Lanzhou China"},{"name":"Gansu Agricultural University\u2013Massey University Research Centre for Grassland Biodiversity Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihong","family":"Ji","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem (Ministry of Education), College of Grassland Science Gansu Agricultural University Lanzhou China"},{"name":"Gansu Agricultural University\u2013Massey University Research Centre for Grassland Biodiversity Gansu Agricultural University  Lanzhou China"},{"name":"School of Biological Sciences University of Auckland Auckland New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5309-1303","authenticated-orcid":false,"given":"Junhu","family":"Su","sequence":"additional","affiliation":[{"name":"Key Laboratory of Grassland Ecosystem (Ministry of Education), College of Grassland Science Gansu Agricultural University Lanzhou China"},{"name":"Gansu Agricultural University\u2013Massey University Research Centre for Grassland Biodiversity Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11020306"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1677\/JOE-07-0017"},{"key":"e_1_2_9_4_1","first-page":"367","article-title":"Research Progress on Toll\u2010Like Receptor 2 (TLR2) with Mycobacterium tuberculosis Infection","volume":"40","author":"Chen R. F.","year":"2024","journal-title":"Chinese Journal of Cellular and Molecular Immunology"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12568"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms221810043"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1000411"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2810359"},{"key":"e_1_2_9_9_1","first-page":"142","article-title":"General Biology of Quinestrol","volume":"12","author":"Giannina T.","year":"1967","journal-title":"International Journal of Fertility"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072282"},{"key":"e_1_2_9_11_1","first-page":"373","article-title":"Regulation of Steroidogenic Function of Mouse Leydig Cells: G\u2010Coupled Membrane Estrogen Receptor and Peroxisome Proliferator\u2010Activated Receptor Partnership","volume":"69","author":"Gorowska\u2010Wojtowicz E.","year":"2018","journal-title":"Journal of Physiology and Pharmacology"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12074"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12623"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10030485"},{"key":"e_1_2_9_15_1","first-page":"259","article-title":"Regulation of Spermatogenesis by Paracrine\/Autocrine Testicular Factors","volume":"6","author":"Huleihel M.","year":"2004","journal-title":"Asian Journal of Andrology"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12588"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/19396368.2013.830234"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14223240"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-78752-1"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12642"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2014.05.012"},{"key":"e_1_2_9_22_1","first-page":"422","article-title":"Protective Effect of Shugan Yiyang Capsule Against Quinestrol\u2010Induced Testicular Disturbances and Oxidative Stress in Male Rats","volume":"50","author":"Li J.","year":"2019","journal-title":"Acta Veterinaria Et Zootechnica Sinica"},{"key":"e_1_2_9_23_1","first-page":"381","article-title":"The Role of MAPK Signaling Pathway in Quinestrol\u2010Induced Apoptosis of Spermatogenic Cells in Rat","volume":"47","author":"Li J.","year":"2016","journal-title":"Acta Veterinaria et Zootechnica Sinica"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2013.09.002"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2009-0148"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12774"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12236"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD10221"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-4877.2012.00296.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv732"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2006.08.064"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12727"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.2478\/bile-2022-0009"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2003.10.020"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/2314808X.2023.2290837"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-023-00822-7"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2021-0132"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2013.03.018"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3733"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2011.10.010"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12733"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2020.02.010"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-061516-045037"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-4877.2007.00067.x"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12598"},{"key":"e_1_2_9_46_1","first-page":"1116","article-title":"Stages and Characteristics of Grassland Rodent Pests Control and Research in China","volume":"30","author":"Su J. 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