{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T02:56:14Z","timestamp":1783047374520,"version":"3.54.6"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Integr. Zool."],"abstract":"ABSTRACT<\/jats:title>\n \n Sex determination and differentiation represent fundamental topics in reproductive biology. Sichuan taimen (\n Hucho bleekeri<\/jats:italic>\n ), a first\u2010class national protected fish species in China, lacks obvious secondary sexual characteristics, making it challenging to distinguish sex without resorting to invasive methods such as dissection. This limitation presents a significant obstacle to both artificial breeding and conservation efforts for the species. Herein, we performed transcriptome and proteome sequencing to identify sex\u2010biased genes of\n H. bleekeri<\/jats:italic>\n . A total of 25\u00a0258 significantly differentially expressed genes (DEGs) were identified in gonadal tissues, including 15\u00a0071 up\u2010regulated male\u2010biased DEGs. Proteomics analysis identified 2937 differentially expressed proteins (DEPs). Integrated analysis identified several key DEGs, including\n sdY<\/jats:italic>\n ,\n CYP11A1<\/jats:italic>\n ,\n 3\u03b2\u2010HSD1<\/jats:italic>\n ,\n CYP17A1<\/jats:italic>\n ,\n CYP11B<\/jats:italic>\n ,\n CYP19A1A<\/jats:italic>\n ,\n 17\u03b2\u2010HSD1<\/jats:italic>\n , and\n 17\u03b2\u2010HSD8<\/jats:italic>\n , in which\n CYP11A1<\/jats:italic>\n ,\n 3\u03b2\u2010HSD1<\/jats:italic>\n ,\n CYP17A1<\/jats:italic>\n , and\n CYP11B<\/jats:italic>\n exhibited high expression in the testis. Furthermore, the cDNA sequence of the\n sdY<\/jats:italic>\n gene was obtained, and in situ hybridization revealed that\n sdY<\/jats:italic>\n is exclusively expressed in the Sertoli cells of the testis. In addition, one pair of\n sdY<\/jats:italic>\n primers was designed to screen sex\u2010linked markers, and the results confirmed that\n sdY<\/jats:italic>\n can serve as a sex\u2010specific marker in\n H. bleekeri<\/jats:italic>\n . The present study would provide a foundation for future research on genes involved in sex determination, differentiation, and artificial breeding in\n H. bleekeri<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1111\/1749-4877.70115","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T08:19:53Z","timestamp":1777450793000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Gonadal Development\u2013Related Genes and a Male\u2010Specific Genetic Marker Identified in the Endangered Sichuan Taimen (\n Hucho bleekeri<\/i>\n )"],"prefix":"10.1111","author":[{"given":"Qinyao","family":"Wei","sequence":"first","affiliation":[{"name":"Key Laboratory of Bio\u2010Resources and Eco\u2010Environment of the Ministry of Education, College of Life Sciences Sichuan University Chengdu China"},{"name":"Fisheries Research Institute Sichuan Academy of Agricultural Sciences Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Li","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute Sichuan Academy of Agricultural Sciences Chengdu China"},{"name":"Observation and Research Station of Sichuan Province of Fish Resources and Environment in Upper Reaches of the Yangtze River Sichuan Academy of Agricultural Sciences Chengdu China"},{"name":"Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute Sichuan Academy of Agricultural Sciences Chengdu China"},{"name":"Observation and Research Station of Sichuan Province of Fish Resources and Environment in Upper Reaches of the Yangtze River Sichuan Academy of Agricultural Sciences Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanchao","family":"Yang","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute Sichuan Academy of Agricultural Sciences Chengdu China"},{"name":"Observation and Research Station of Sichuan Province of Fish Resources and Environment in Upper Reaches of the Yangtze River Sichuan Academy of Agricultural Sciences Chengdu China"},{"name":"Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Du","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute Sichuan Academy of Agricultural Sciences Chengdu China"},{"name":"Observation and Research Station of Sichuan Province of Fish Resources and Environment in Upper Reaches of the Yangtze River Sichuan Academy of Agricultural Sciences Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaobin","family":"Song","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio\u2010Resources and Eco\u2010Environment of the Ministry of Education, College of Life Sciences Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","DOI":"10.1098\/rstb.2020.0092","article-title":"Lessons from an Unusual Vertebrate Sex\u2010Determining Gene","volume":"376","author":"Bertho S.","year":"2021","journal-title":"Philosophical Transactions of the Royal Society B: Biological Sciences"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.cbpa.2010.01.006","article-title":"Molecular Characterization of cyp11a1 and cyp11b1 and Their Gene Expression Profile in Pejerrey (Odontesthes bonariensis) During Early Gonadal Development","volume":"156","author":"Blasco M.","year":"2010","journal-title":"Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-80651-4","article-title":"A Study on the Functional Role of the DHCR24 Gene in Gonadal Differentiation and Development of Macrobrachium nipponense","volume":"14","author":"Cai P.","year":"2024","journal-title":"Scientific Reports"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1038\/nrg.2017.60","article-title":"Vertebrate Sex Determination: Evolutionary Plasticity of a Fundamental Switch","volume":"18","author":"Capel B.","year":"2017","journal-title":"Nature Reviews Genetics"},{"key":"e_1_2_10_6_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2020.103934","article-title":"Full\u2010Length Transcriptome Sequencing and Identification of Immune\u2010Related Genes in the Critically Endangered Hucho bleekeri","volume":"116","author":"Chen Y.","year":"2021","journal-title":"Developmental and Comparative Immunology"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0301884","article-title":"Proteome Analysis Provides Insights into Sex Differences in Holothuria scabra","volume":"19","author":"Cheng C.","year":"2024","journal-title":"PLoS ONE"},{"key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"855","DOI":"10.15252\/msb.20156423","article-title":"Differential Dynamics of the Mammalian mRNA and Protein Expression Response to Misfolding Stress","volume":"12","author":"Cheng Z.","year":"2016","journal-title":"Molecular Systems Biology"},{"key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1126\/science.aap8328","article-title":"The Histone Demethylase KDM6B Regulates Temperature\u2010Dependent Sex Determination in a Turtle Species","volume":"360","author":"Ge C.","year":"2018","journal-title":"Science"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1002\/(SICI)1098-2795(199910)54:2<154::AID-MRD7>3.0.CO;2-5","article-title":"Involvement of Estrogens in the Process of Sex Differentiation in Two Fish Species: The Rainbow Trout (Oncorhynchus mykiss) and a Tilapia (Oreochromis niloticus)","volume":"54","author":"Guiguen Y.","year":"1999","journal-title":"Molecular Reproduction and Development"},{"key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"2955","DOI":"10.1073\/pnas.1018392109","article-title":"A Y\u2010Linked Anti\u2010M\u00fcllerian Hormone Duplication Takes over a Critical Role in Sex Determination","volume":"109","author":"Hattori R. S.","year":"2012","journal-title":"PNAS"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2022.739130","article-title":"Multi\u2010Locus Gene Editing Effectively Knocked Out cyp19a1a and foxl2 in Monopterus albus, a Hermaphroditic Fish","volume":"565","author":"Hou M.","year":"2023","journal-title":"Aquaculture"},{"key":"e_1_2_10_13_1","doi-asserted-by":"crossref","first-page":"2978","DOI":"10.1210\/endo.142.7.8244","article-title":"Cholesterol and Hydroxycholesterol Sulfotransferases: Identification, Distinction from Dehydroepiandrosterone Sulfotransferase, and Differential Tissue Expression","volume":"142","author":"Javitt N. B.","year":"2001","journal-title":"Endocrinology"},{"key":"e_1_2_10_14_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1002798","article-title":"A Trans\u2010Species Missense SNP in Amhr2 Is Associated with Sex Determination in the Tiger Pufferfish, Takifugu rubripes (Fugu)","volume":"8","author":"Kamiya T.","year":"2012","journal-title":"PLoS Genetics"},{"key":"e_1_2_10_15_1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jsbmb.2005.12.004","article-title":"Cloning and Characterization of a cDNA Encoding Cholesterol Side\u2010Chain Cleavage Cytochrome P450 (CYP11A1): Tissue\u2010Distribution and Changes in the Transcript Abundance in Ovarian Tissue of Japanese Eel, Anguilla japonica, During Artificially Induced Sexual Development","volume":"99","author":"Kazeto Y.","year":"2006","journal-title":"Journal of Steroid Biochemistry and Molecular Biology"},{"key":"e_1_2_10_16_1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1146\/annurev-animal-021122-113935","article-title":"Diversity and Convergence of Sex\u2010Determination Mechanisms in Teleost Fish","volume":"12","author":"Kitano J.","year":"2024","journal-title":"Annual Review of Animal Biosciences"},{"key":"e_1_2_10_17_1","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1007\/s002270050577","article-title":"Sexual Maturity and Hermaphroditism of the Red Porgy Pagrus pagrus (Teleostei: Sparidae)","volume":"134","author":"Kokokiris L.","year":"1999","journal-title":"Marine Biology"},{"key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1007882807782","article-title":"Endocrine Changes During the Annual Reproductive Cycle of the Red Porgy, Pagrus pagrus (Teleostei, Sparidae)","volume":"23","author":"Kokokiris L.","year":"2000","journal-title":"Fish Physiology and Biochemistry"},{"key":"e_1_2_10_19_1","doi-asserted-by":"crossref","DOI":"10.1038\/srep37357","article-title":"Knockout of Zebrafish Ovarian Aromatase Gene (cyp19a1a) by TALEN and CRISPR\/Cas9 Leads to All\u2010Male Offspring due to Failed Ovarian Differentiation","volume":"6","author":"Lau E. S.","year":"2016","journal-title":"Scientific Reports"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1005678","article-title":"A Tandem Duplicate of Anti\u2010M\u00fcllerian Hormone with a Missense SNP on the Y Chromosome Is Essential for Male Sex Determination in Nile Tilapia, Oreochromis niloticus","volume":"11","author":"Li M.","year":"2015","journal-title":"PLoS Genetics"},{"key":"e_1_2_10_21_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2024.741258","article-title":"Transcriptomic Analysis of Gonads in Malabar Red Snapper (Lutjanus malabaricus) Reveals Genes Associated with Gonad Development","volume":"592","author":"Liang B.","year":"2024","journal-title":"Aquaculture"},{"key":"e_1_2_10_22_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real\u2010Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak K. J.","year":"2001","journal-title":"Methods"},{"key":"e_1_2_10_23_1","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/nature751","article-title":"DMY Is a Y\u2010Specific DM\u2010Domain Gene Required for Male Development in the Medaka Fish","volume":"417","author":"Matsuda M.","year":"2002","journal-title":"Nature"},{"key":"e_1_2_10_24_1","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.gene.2012.10.064","article-title":"Cloning and Expression Analysis of the Cytochrome P450c17s Enzymes During the Reproductive Cycle in Ovoviviparous Korean Rockfish (Sebastes schlegeli)","volume":"512","author":"Mu W. J.","year":"2013","journal-title":"Gene"},{"key":"e_1_2_10_25_1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1534\/genetics.111.137497","article-title":"Tracing the Emergence of a Novel Sex\u2010Determining Gene in Medaka, Oryzias luzonensis","volume":"191","author":"Myosho T.","year":"2012","journal-title":"Genetics"},{"key":"e_1_2_10_26_1","doi-asserted-by":"crossref","first-page":"11778","DOI":"10.1073\/pnas.182314699","article-title":"A Duplicated Copy of DMRT1 in the Sex\u2010Determining Region of the Y Chromosome of the Medaka, Oryzias latipes","volume":"99","author":"Nanda I.","year":"2002","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"e_1_2_10_27_1","doi-asserted-by":"crossref","first-page":"6514","DOI":"10.1021\/acsomega.7b00577","article-title":"Unraveling the Stability of Plasma Proteins upon Interaction of Synthesized Androstenedione and Its Derivatives\u2014A Biophysical and Computational Approach","volume":"2","author":"Nerusu A.","year":"2017","journal-title":"ACS Omega"},{"key":"e_1_2_10_28_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1159\/000328823","article-title":"Dimorphic Expression of Various Transcription Factor and Steroidogenic Enzyme Genes During Gonadal Ontogeny in the Air\u2010Breathing Catfish, Clarias gariepinus","volume":"5","author":"Raghuveer K.","year":"2011","journal-title":"Sexual Development"},{"key":"e_1_2_10_29_1","doi-asserted-by":"crossref","first-page":"17926","DOI":"10.3390\/ijms140917926","article-title":"Regulation of 3\u03b2\u2010Hydroxysteroid Dehydrogenase\/\u03945\u2010\u03944 Isomerase: A Review","volume":"14","author":"Rasmussen M. K.","year":"2013","journal-title":"International Journal of Molecular Sciences"},{"key":"e_1_2_10_30_1","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1186\/s12864-025-11375-6","article-title":"A Detailed Transcriptome Study Uncovers the Epigenetic Characteristics Associated with Aromatase Inhibitor\u2010Induced Masculinization in Takifugu rubripes Larvae Gonads","volume":"26","author":"Shen X.","year":"2025","journal-title":"BMC Genomics"},{"key":"e_1_2_10_31_1","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1038\/346240a0","article-title":"A Gene from the Human Sex\u2010Determining Region Encodes a Protein with Homology to a Conserved DNA\u2010Binding Motif","volume":"346","author":"Sinclair A. H.","year":"1990","journal-title":"Nature"},{"key":"e_1_2_10_32_1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nature08298","article-title":"The Avian Z\u2010Linked Gene DMRT1 Is Required for Male Sex Determination in the Chicken","volume":"461","author":"Smith C. A.","year":"2009","journal-title":"Nature"},{"key":"e_1_2_10_33_1","doi-asserted-by":"crossref","first-page":"4155","DOI":"10.1038\/s41467-018-06697-x","article-title":"The H3K9 Methyltransferase SETDB1 Maintains Female Identity in Drosophila Germ Cells","volume":"9","author":"Smolko A. E.","year":"2018","journal-title":"Nature Communications"},{"key":"e_1_2_10_34_1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1677\/joe.0.1640247","article-title":"The Role of the StAR Protein in Steroidogenesis: Challenges for the Future","volume":"164","author":"Stocco D. M.","year":"2000","journal-title":"Journal of Endocrinology"},{"key":"e_1_2_10_35_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0003-9861(02)00547-7","article-title":"Ovine Steroid 17\u03b1\u2010hydroxylase Cytochrome P450: Characteristics of the Hydroxylase and Lyase Activities of the Adrenal Cortex Enzyme","volume":"409","author":"Swart P.","year":"2003","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"e_1_2_10_36_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aqrep.2025.102761","article-title":"Regulatory Patterns of Testis and Ovary Maturation in Largemouth Bass (Micropterus salmoides) Revealed by Transcriptome Profiling","volume":"42","author":"Wang Q.","year":"2025","journal-title":"Aquaculture Reports"},{"key":"e_1_2_10_37_1","doi-asserted-by":"crossref","DOI":"10.1098\/rstb.2020.0109","article-title":"Sex Determination Without Sex Chromosomes","volume":"376","author":"Weber C.","year":"2021","journal-title":"Philosophical Transactions of the Royal Society of London B: Biological Sciences"},{"key":"e_1_2_10_38_1","doi-asserted-by":"crossref","DOI":"10.1111\/jfd.14168","article-title":"The Pilot Study on the Histological and Ultrastructural Characteristics of Major Immune Organs (Spleen, Head Kidney, and Trunk Kidney) and Analysis of Pathological Features in Sichuan Taimen (Hucho bleekeri)","volume":"48","author":"Wei Q.","year":"2025","journal-title":"Journal of Fish Diseases"},{"key":"e_1_2_10_39_1","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.3390\/genes15101314","article-title":"Metagenomic Sequencing Elucidated the Microbial Diversity of Rearing Water Environments for Sichuan Taimen (Hucho bleekeri)","volume":"15","author":"Wei Q.","year":"2024","journal-title":"Genes (Basel)"},{"key":"e_1_2_10_40_1","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.ygcen.2017.01.033","article-title":"Primary Males Guide the Femaleness Through the Regulation of Testicular Dmrt1 and Ovarian Cyp19a1a in Protandrous Black Porgy","volume":"261","author":"Wu G. C.","year":"2018","journal-title":"General and Comparative Endocrinology"},{"key":"e_1_2_10_41_1","doi-asserted-by":"crossref","DOI":"10.1155\/jai\/5526431","article-title":"Cloning, Expression and Stability Analysis of Candidate Reference Genes in the Glacial Relict Hucho bleekeri Under Immune Stress","volume":"2025","author":"Wu X.","year":"2025","journal-title":"Journal of Applied Ichthyology"},{"key":"e_1_2_10_42_1","doi-asserted-by":"crossref","DOI":"10.1210\/endocr\/bqab205","article-title":"Cyp17a1 is Required for Female Sex Determination and Male Fertility by Regulating Sex Steroid Biosynthesis in Fish","volume":"162","author":"Yang L.","year":"2021","journal-title":"Endocrinology"},{"key":"e_1_2_10_43_1","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.cub.2012.05.045","article-title":"An Immune\u2010Related Gene Evolved into the Master Sex\u2010Determining Gene in Rainbow Trout, Oncorhynchus mykiss","volume":"22","author":"Yano A.","year":"2012","journal-title":"Current Biology"},{"key":"e_1_2_10_44_1","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1111\/eva.12032","article-title":"The Sexually Dimorphic on the Y\u2010Chromosome Gene (sdY) Is a Conserved Male\u2010Specific Y\u2010Chromosome Sequence in Many Salmonids","volume":"6","author":"Yano A.","year":"2013","journal-title":"Evolutionary Applications"},{"key":"e_1_2_10_45_1","doi-asserted-by":"crossref","first-page":"2519","DOI":"10.1242\/dev.048751","article-title":"Opposite Roles of DMRT1 and Its W\u2010Linked Paralogue, DM\u2010W, in Sexual Dimorphism of Xenopus laevis: Implications of a ZZ\/ZW\u2010Type Sex\u2010Determining System","volume":"137","author":"Yoshimoto S.","year":"2010","journal-title":"Development"},{"key":"e_1_2_10_46_1","doi-asserted-by":"crossref","first-page":"2444","DOI":"10.1007\/s11427-023-2694-5","article-title":"Unveiling Potential Sex\u2010Determining Genes and Sex\u2010Specific Markers in Autotetraploid Carassius auratus","volume":"67","author":"Zhang K.","year":"2024","journal-title":"Science China Life Sciences"}],"container-title":["Integrative Zoology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1749-4877.70115","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1749-4877.70115","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1749-4877.70115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T08:19:59Z","timestamp":1777450799000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1749-4877.70115"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,29]]},"references-count":45,"alternative-id":["10.1111\/1749-4877.70115"],"URL":"https:\/\/doi.org\/10.1111\/1749-4877.70115","archive":["Portico"],"relation":{},"ISSN":["1749-4877","1749-4877"],"issn-type":[{"value":"1749-4877","type":"print"},{"value":"1749-4877","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,29]]},"assertion":[{"value":"2025-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1749-4877.70115"}}