{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:54:56Z","timestamp":1772996096689,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,3,11]],"date-time":"2013-03-11T00:00:00Z","timestamp":1362960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Coagulase\u2010negative staphylococci (CoNS), which are generally neglected as foodborne bacteria, are emerging as significant opportunistic pathogens that may be highly resistant to available antimicrobial drugs. In this study, antimicrobial susceptibility patterns,\n                    <jats:italic>mecA<\/jats:italic>\n                    gene occurrence, and virulence\u2010associated characteristics were evaluated in CoNS isolated from soft cheese in Brazil. A total of 227 bacterial isolates were recovered from 35 cheese samples belonging to 5 batches with 7 different trademarks. The CoNS counts ranged from 10\n                    <jats:sup>6<\/jats:sup>\n                    to 10\n                    <jats:sup>7<\/jats:sup>\n                    CFU\/g. High antimicrobial resistance percentages were observed for oxacillin (76.2%), penicillin (78.5%), erythromycin (67.8%), gentamicin (47.2%), clindamycin (35.7%), rifampicin (26.8%), azithromycin (14.7%), tetracycline (14.7%), levofloxacin (14.2%), and sulfamethoxazole\u2010trimethoprim (11.9%). A low antimicrobial resistance percentage was observed for chloramphenicol (2.3%), and all of the tested bacteria were susceptible to vancomycin and linezolid. In total, a multiple antibiotic resistance (MAR) index of &gt;0.2 was observed for 80.6% of the isolated CoNS. However, the MAR index ranged from 50% to 92.6% when only bacterial cheese isolates belonging to the same trademark were considered. Regarding to the prevalence of CoNS carrying\n                    <jats:italic>mecA<\/jats:italic>\n                    gene, 81.5% of the isolated strains were\n                    <jats:italic>mecA<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    , and 76.2% of these were phenotypically resistant to oxacillin. Three isolates carried the\n                    <jats:italic>enterotoxin A<\/jats:italic>\n                    gene (\n                    <jats:italic>sea<\/jats:italic>\n                    ), 29.5% produced biofilm in a laboratory test, and \u03b1\u2010 or \u00df\u2010hemolysis were observed for 3% and 5.2%, respectively. This study highlights the extent of the antimicrobial resistance phenomenon in neglected foodborne microorganisms and the potential public health risks that are related to the consumption of CoNS\u2010contaminated soft cheese.\n                  <\/jats:p>","DOI":"10.1111\/1750-3841.12088","type":"journal-article","created":{"date-parts":[[2013,3,13]],"date-time":"2013-03-13T16:02:19Z","timestamp":1363190539000},"source":"Crossref","is-referenced-by-count":20,"title":["Prevalence, Antimicrobial Resistance, and Virulence Characteristics of\n                    <i>mecA<\/i>\n                    \u2010Encoding Coagulase\u2010Negative Staphylococci Isolated from Soft Cheese in Brazil"],"prefix":"10.1111","volume":"78","author":[{"given":"Cl\u00e1udia Oliveira","family":"Fontes","sequence":"first","affiliation":[{"name":"Dept. of Parasitology Microbiology and Immunology, Federal Univ. of Juiz de Fora  Juiz de Fora Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e2nia L\u00facia","family":"Silva","sequence":"additional","affiliation":[{"name":"Dept. of Parasitology Microbiology and Immunology, Federal Univ. of Juiz de Fora  Juiz de Fora Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayara Rodrigues Brand\u00e3o","family":"de Paiva","sequence":"additional","affiliation":[{"name":"Dept. of Parasitology Microbiology and Immunology, Federal Univ. of Juiz de Fora  Juiz de Fora Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafaela Alvim","family":"Garcia","sequence":"additional","affiliation":[{"name":"Dept. of Parasitology Microbiology and Immunology, Federal Univ. of Juiz de Fora  Juiz de Fora Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Alves","family":"Resende","sequence":"additional","affiliation":[{"name":"Dept. of Parasitology Microbiology and Immunology, Federal Univ. of Juiz de Fora  Juiz de Fora Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra Barbosa","family":"Ferreira\u2010Machado","sequence":"additional","affiliation":[{"name":"Dept. of Parasitology Microbiology and Immunology, Federal Univ. of Juiz de Fora  Juiz de Fora Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udio Galuppo","family":"Diniz","sequence":"additional","affiliation":[{"name":"Dept. of Parasitology Microbiology and Immunology, Federal Univ. of Juiz de Fora  Juiz de Fora Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,3,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms10041538"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0255-0857(21)01748-5","article-title":"Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA","volume":"27","author":"Anand KB","year":"2009","journal-title":"Indian J Med Microbiol"},{"key":"e_1_2_7_4_1","first-page":"826","volume-title":"Science against microbial pathogens: communicating current research and technological advances","author":"Ara\u00fajo P","year":"2011"},{"key":"e_1_2_7_5_1","volume-title":"Report from the American Academy of Microbiology","author":"[ASM] American Society for Microbiology","year":"2009"},{"key":"e_1_2_7_6_1","unstructured":"BRASIL.2001.Minist\u00e9rio da Sa\u00fade \u2013 Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria (ANVISA). 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