{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T15:36:52Z","timestamp":1762184212323,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T00:00:00Z","timestamp":1402358400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2014,6]]},"abstract":"Abstract<\/jats:title>\n \n This study evaluated the genetic similarity and prevalence of the\n stx1<\/jats:italic>\n ,\n stx2<\/jats:italic>\n ,\n eae<\/jats:italic>\n , and\n ehxA<\/jats:italic>\n genes in\n Escherichia coli<\/jats:italic>\n isolated from pasteurized cow milk. Eighty\u2010seven\n E. coli<\/jats:italic>\n isolates from pasteurized cow milk from 22 dairies located in northwestern Paran\u00e1 state, Brazil, were analyzed. Genetic similarity was evaluated using enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC\u2010PCR) and repetitive extragenic palindromic sequence PCR (REP\u2010PCR).\n E. coli<\/jats:italic>\n isolates were also analyzed by PCR to investigate the presence of the\n stx1<\/jats:italic>\n ,\n stx2<\/jats:italic>\n ,\n eae<\/jats:italic>\n , and\n ehxA<\/jats:italic>\n genes. ERIC\u2010PCR and REP\u2010PCR clustered 87 bacterial isolates in 76 and 81 genomic profiles, respectively. Both techniques revealed high genetic diversity among the\n E. coli<\/jats:italic>\n isolates, confirming the possibility of their use in epidemiological studies. The\n stx1<\/jats:italic>\n ,\n stx2<\/jats:italic>\n ,\n eae<\/jats:italic>\n , and\n ehxA<\/jats:italic>\n virulence genes were not detected in\n E. coli<\/jats:italic>\n isolates, indicating a low prevalence of Shiga toxin\u2010producing\n E. coli<\/jats:italic>\n in milk produced in the region studied.\n <\/jats:p>","DOI":"10.1111\/1750-3841.12477","type":"journal-article","created":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T20:20:23Z","timestamp":1402431623000},"source":"Crossref","is-referenced-by-count":9,"title":["Shiga\u2010Toxin Genes and Genetic Diversity of\n Escherichia coli<\/i>\n Isolated from Pasteurized Cow Milk in Brazil"],"prefix":"10.1111","volume":"79","author":[{"given":"Simone Aparecida","family":"Hoffmann","sequence":"first","affiliation":[{"name":"Graduate Program in Food Sciences Center of Agrarian Sciences State Univ. of Maring\u00e1 Colombo Ave. 5790, Block J\u201045 Maring\u00e1 Paran\u00e1 87020\u2013900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriella Giani","family":"Pieretti","sequence":"additional","affiliation":[{"name":"Graduate Program in Food Sciences Center of Agrarian Sciences State Univ. of Maring\u00e1 Colombo Ave. 5790, Block J\u201045 Maring\u00e1 Paran\u00e1 87020\u2013900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Fiorini","sequence":"additional","affiliation":[{"name":"Federal Univ. of Paran\u00e1 Pioneiro Street 2153 Palotina Paran\u00e1 CEP 85950\u2013970 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliana Val\u00e9ria","family":"Patussi","sequence":"additional","affiliation":[{"name":"Dept. of Clinical Analyses and Biomedicine State Univ. of Maring\u00e1 Colombo Ave. 5790, Block T20 Maring\u00e1 Paran\u00e1 87020\u2013900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosilene Fressatti","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Dept. of Clinical Analyses and Biomedicine State Univ. of Maring\u00e1 Colombo Ave. 5790, Block T20 Maring\u00e1 Paran\u00e1 87020\u2013900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane Martha Graton","family":"Mikcha","sequence":"additional","affiliation":[{"name":"Graduate Program in Food Sciences Center of Agrarian Sciences State Univ. of Maring\u00e1 Colombo Ave. 5790, Block J\u201045 Maring\u00e1 Paran\u00e1 87020\u2013900 Brazil"},{"name":"Dept. of Clinical Analyses and Biomedicine State Univ. of Maring\u00e1 Colombo Ave. 5790, Block T20 Maring\u00e1 Paran\u00e1 87020\u2013900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,6,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2009.01.005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.311-319.2004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822007000300032"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2008.05.017"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2005.10.010"},{"key":"e_1_2_7_7_1","unstructured":"CDC (Centers for Disease Control and Prevention).2012.Escherichia coliO157:H7 and other Shiga toxin\u2010producingEscherichia coli(STEC). 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