{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:59:43Z","timestamp":1762271983547,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,11,20]],"date-time":"2015-11-20T00:00:00Z","timestamp":1447977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Staphylococ<\/jats:italic>\n                    c\n                    <jats:italic>us aureus<\/jats:italic>\n                    (\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ) can cause mastitis in cattle and, therefore, can be present in milk. This study was undertaken to determine the prevalence of coagulase positive\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and its enterotoxin genes\n                    <jats:italic>sea, seb<\/jats:italic>\n                    , and\n                    <jats:italic>sec<\/jats:italic>\n                    in isolates recovered from raw milk, feta cheese, and human hand swabs of milk and cheese handlers in Beni\u2010Suef province, Egypt. A total of 100 samples of raw milk and 50 samples of pasteurized\u2010milk feta cheese were collected. In addition, 50 hand swabs from milk handlers and 25 hand swabs from cheese handlers were examined for the presence of coagulase positive\n                    <jats:italic>S. aureus<\/jats:italic>\n                    . The isolates were characterized by multiplex PCR for detection of\n                    <jats:italic>sea<\/jats:italic>\n                    ,\n                    <jats:italic>seb<\/jats:italic>\n                    , and\n                    <jats:italic>sec<\/jats:italic>\n                    genes, and for resistance to 5 classes of commonly used antibiotics. Twelve (12\/100), 12 (6\/50), and 17% (13\/75) of milk, cheese, and hand swab samples, respectively, were positive for coagulase positive\n                    <jats:italic>S. aureus<\/jats:italic>\n                    . One isolate was obtained from each positive sample (31 isolates), and none contained genes for SEA or SEC production. Twenty\u2010five percent, 33%, and 31%, respectively, of the isolates contained the genes for SEB, resulting in 3%, 4%, and 5% of samples being positive for toxin producing coagulase positive\n                    <jats:italic>S. aureus<\/jats:italic>\n                    , respectively. At least one isolate was resistant to each of the antibiotics tested. Despite the low potential for SEB production shown, preventative measures, such as maintenance of the cold\u2010chain and good hygienic practices should be implemented to further reduce the potential risk to public health from SEB, and to reduce the spread of antimicrobial resistance.\n                  <\/jats:p>","DOI":"10.1111\/1750-3841.13147","type":"journal-article","created":{"date-parts":[[2015,11,20]],"date-time":"2015-11-20T15:18:28Z","timestamp":1448032708000},"source":"Crossref","is-referenced-by-count":17,"title":["The Use of Multiplex PCR to Determine the Prevalence of Enterotoxigenic\n                    <i>Staphylococcus aureus<\/i>\n                    Isolated from Raw Milk, Feta Cheese, and Hand Swabs"],"prefix":"10.1111","volume":"80","author":[{"given":"Mohamed M.A.","family":"Zeinhom","sequence":"first","affiliation":[{"name":"Food Hygiene &amp; Control Dept., Faculty of Veterinary Medicine Beni\u2010Suef Univ  Beni\u2010Suef 62512 Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gihan K.","family":"Abdel\u2010Latef","sequence":"additional","affiliation":[{"name":"Hygiene, Management and Zoonoses Dept., Faculty of Veterinary Medicine BeniSuef Univ  Beni\u2010Suef 62512 Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kieran","family":"Jordan","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Centre Moorepark  Fermoy, Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,11,20]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"229","article-title":"Enterotoxigenic Staphylococcus aureus in raw milk in the north of Palestine","volume":"29","author":"Adwan G","year":"2005","journal-title":"Turk J Biol"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2007.03.010"},{"key":"e_1_2_5_4_1","first-page":"826","article-title":"Detection of Staphylococcus aureus enterotoxins A to E from clinical sample by PCR","volume":"3","author":"Anvari SH","year":"2008","journal-title":"Res J Biol Sci"},{"volume-title":"Standard methods for examination of dairy products","year":"1992","author":"APHA","key":"e_1_2_5_5_1"},{"volume-title":"Compendium of methods for the microbiological examination of food","year":"2004","author":"APHA","key":"e_1_2_5_6_1"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07487-11"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268802007951"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3869-3872.2004"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1962.tb01113.x"},{"key":"e_1_2_5_11_1","first-page":"83","article-title":"Pitfalls in the routine diagnosis of Staphylococcus aureus","volume":"4","author":"Bello CSS","year":"2005","journal-title":"Afr J Biotechnol"},{"key":"e_1_2_5_12_1","first-page":"463","volume-title":"Staphylococcus aureus","author":"Bergdoll MS.","year":"1989"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02349.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-7135(02)00109-3"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.2.232-260.2001"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/09603120120110031"},{"volume-title":"Performance standards for antimicrobial susceptibility testing; 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