{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T09:34:59Z","timestamp":1771148099924,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T00:00:00Z","timestamp":1514505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31701715"],"award-info":[{"award-number":["31701715"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Human norovirus (HuNoV) is a major foodborne virus causing gastroenteritis outbreaks in humans. Salad products can be vectors of transmission for foodborne viruses such as HuNoV when these products are contaminated naturally or through unsanitary food handling. Therefore, development of simple, reliable and sensitive techniques for the detection of HuNoV in salad products is needed to ensure food safety. The purpose of our study was to optimize a method for the detection of HuNoV in artificially contaminated salad products. To this end, 2 different kinds of salads (fruit salads and vegetable salads) were experimentally inoculated with HuNoV GI, HuNoV GII, and MS2 suspensions. The selected method was based on treatment with pectinase followed by Trizol\u2010chloroform purification, and the recovery efficiencies were 6.07% to 26.52% for HuNoV GI and 5.54% to 37.36% for HuNoV GII. MS2 was used as the process control, and the recovery efficiencies for fruit salad and vegetable salad samples were 38.57% and 41.13%, respectively. The optimized method could be applied in diagnostic laboratories to identify NoV contamination in composite foods, such as salad products, should an event of foodborne outbreak occur.<\/jats:p>","DOI":"10.1111\/1750-3841.14022","type":"journal-article","created":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T18:16:39Z","timestamp":1514571399000},"page":"393-400","source":"Crossref","is-referenced-by-count":18,"title":["Evaluation of an Extraction Method for the Detection of GI and GII Noroviruses in Fruit and Vegetable Salads"],"prefix":"10.1111","volume":"83","author":[{"given":"Dongqing","family":"Cheng","sequence":"first","affiliation":[{"name":"Coll. of Medical Technology Zhejiang Chinese Medical Univ.  Hangzhou 310053 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songyan","family":"Zou","sequence":"additional","affiliation":[{"name":"Dept. of Nutrition and Food Safety Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou 310006 China"},{"name":"Coll. of Medical Technology Zhejiang Chinese Medical Univ.  Hangzhou 310053 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1914-2319","authenticated-orcid":false,"given":"Ningbo","family":"Liao","sequence":"additional","affiliation":[{"name":"Dept. of Nutrition and Food Safety Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou 310006 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofeng","family":"Shi","sequence":"additional","affiliation":[{"name":"Coll. of Medical Technology Zhejiang Chinese Medical Univ.  Hangzhou 310053 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Dept. of Nutrition and Food Safety Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou 310006 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Dept. of Nutrition and Food Safety Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou 310006 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Sun","sequence":"additional","affiliation":[{"name":"Dept. of Nutrition and Food Safety Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou 310006 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronghua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Dept. of Nutrition and Food Safety Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou 310006 China"},{"name":"Coll. of Medical Technology Zhejiang Chinese Medical Univ.  Hangzhou 310053 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01248-06"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.12.020"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.02.017"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2014.1821"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.05.003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-65.12.1962"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.02.026"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12161-011-9262-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1969.tb10330.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2016.09.001"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2012.12.007"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis251"},{"key":"e_1_2_7_14_1","first-page":"491","article-title":"Vital signs: foodborne norovirus outbreaks\u2014United States, 2009\u20132012","volume":"63","author":"Hall AJ.","year":"2014","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2009.03.020"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2016.10.021"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750-3841.12462"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2013.06.035"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02576-13"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03538-15"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.2217\/fvl.13.128"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2014.01.042"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2007.08.024"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2011.12.026"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2007.05.005"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.06.008"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2012.04.006"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni176"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2011.10.014"},{"key":"e_1_2_7_30_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.3920\/QAS2013.0250","article-title":"A comparison of proteinase K and PEG8000 on the recovery of calicivirus and norovirus in artificially contaminated food","volume":"7","author":"Tibollo S","year":"2014","journal-title":"Qual Assur Saf Crop"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/345036a0"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2012.11.038"},{"key":"e_1_2_7_33_1","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1007\/s00217-014-2272-2","article-title":"Detection of GI and GII noroviruses in drinking water and vegetables using filtration and real\u2010time RT\u2010PCR","volume":"239","author":"Xu D","year":"2014","journal-title":"Eur Food Res Technol"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750-3841.12899"}],"container-title":["Journal of Food Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1750-3841.14022","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.14022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T11:04:30Z","timestamp":1762167870000},"score":1,"resource":{"primary":{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/10.1111\/1750-3841.14022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,29]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/1750-3841.14022"],"URL":"https:\/\/doi.org\/10.1111\/1750-3841.14022","archive":["Portico"],"relation":{},"ISSN":["0022-1147","1750-3841"],"issn-type":[{"value":"0022-1147","type":"print"},{"value":"1750-3841","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,29]]}}}