{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:48:18Z","timestamp":1788472098530,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,9,14]],"date-time":"2018-09-14T00:00:00Z","timestamp":1536883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Portuguese Foundation for Science and Technology (FCT) in the project INNOFOOD - INNOvation in the FOOD","award":["NORTE-07-0124-FEDER-0000029"],"award-info":[{"award-number":["NORTE-07-0124-FEDER-0000029"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["BI\/INNOFOOD\/UTAD\/256\/2014"],"award-info":[{"award-number":["BI\/INNOFOOD\/UTAD\/256\/2014"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["SFRH\/BPD\/70378\/2010"],"award-info":[{"award-number":["SFRH\/BPD\/70378\/2010"]}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      The need to support food labelling has driven to the development of PCR\u2010based techniques suitable for food analysis. DNA\u2010based markers have been successfully employed for varietal tracing in Protected Designation of Origin (PDO) olive oils. In this study, we report a fast, sensitive, and easy\u2010to\u2010use strategy for PDO olive varietal identification. To achieve this aim four different DNA extraction methods were tested and compared, based on initial volume, extraction time, the gDNA concentration, and quality ratios. The optimized DNA extraction protocol from extra virgin olive oils, based on CTAB\u2013hexane\u2013chloroform extraction, proved to be the most effective. High\u2010resolution melting (HRM) DNA assay was developed based on nuclear microsatellites (gSSR) and plastid DNA (cpDNA) aiming an accurate identification of the olive varieties present in the olive oil samples. After PCR reproducibility evaluation, six molecular markers: three SSRs and three cpDNA\n                      <jats:italic>loci<\/jats:italic>\n                      were chosen based on their discrimination power. The SSR\u2010HRM strategy assays were designed to target\n                      <jats:italic>UDO99\u2010011<\/jats:italic>\n                      ,\n                      <jats:italic>UDO99\u2010039, UDO99\u2010024<\/jats:italic>\n                      , and\n                      <jats:italic>ssrOeUA\u2010DCA16 loci<\/jats:italic>\n                      . All SSR\u2010PCR products generated from these primers were analyzed by capillary electrophoresis (CE) for HRM data validation. The SSR coupled with HRM melting curve analysis generated 14 HRM profiles sufficient to genotype all varieties, highlighting their potential use for varietal discrimination. The\n                      <jats:italic>locus ssrOeUA\u2010DCA16<\/jats:italic>\n                      generated a specific melting curve that allow a high\u2010throughput discrimination of the Picual and Cobran\u00e7osa varieties in olive oil samples. Further, the\n                      <jats:italic>UDO99\u2010024<\/jats:italic>\n                      was also tested by SSR\u2010HRM assay in commercial olive oil samples with promising results. Considering time, cost, and performance SSR\u2010HRM proved to be a reliable method suitable for varietal tracing of olive oils.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Application<\/jats:title>\n                    <jats:p>Olive oil authenticity is a form of protecting producers and consumers against fraudulent practices. Herein, we present a DNA barcode suitable for the identification of olive varieties, allowing an accurate identification of the olive varieties in olive oil samples using SSR\u2010HRM assay. Its applicability in commercial olive oil samples is viable. This methodology can be used as a tool for Extra Virgin Olive Oil (EVOO) adulterations detection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.14333","type":"journal-article","created":{"date-parts":[[2018,9,16]],"date-time":"2018-09-16T06:31:32Z","timestamp":1537079492000},"page":"2415-2423","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Microsatellite High\u2010Resolution Melting (SSR\u2010HRM) to Track Olive Genotypes: From Field to Olive Oil"],"prefix":"10.1111","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3536-0300","authenticated-orcid":false,"given":"S\u00f3nia","family":"Gomes","sequence":"first","affiliation":[{"name":"School of Life Science and Environment, Dept. of Genetics and Biotechnology, Blocos Laboratoriais bdg Univ. of Tr\u00e1s\u2010os\u2010Montes and Alto Douro  5000\u2013801 Vila Real Portugal"},{"name":"Faculty of Sciences, BioISI \u2010 Biosystems &amp; Integrative Sciences Inst. Campo Grande Univ. of Lisboa  C8 bdg 1749\u2010016 Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Breia","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro\u2010Environmental and Biological Sciences  CITAB 5000\u2013801 Vila Real Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Natl. Inst. for Agricultural and Veterinary Research (INIAV)  P.O. 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