{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:13:56Z","timestamp":1762175636520,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T00:00:00Z","timestamp":1560902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017R1A2B4009097"],"award-info":[{"award-number":["2017R1A2B4009097"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Orostachys japonicus<\/jats:italic>\n                      has traditionally been used as a food product and a fork medicine in Asia to treat various diseases. Angiogenesis is a critical process that contributes to various chronic diseases via excessive delivery of oxygen and nutrients. Common anti\u2010angiogenic drugs have serious problems related to high costs and side effects; thus, natural products with low costs and no cytotoxicity have garnered increasing interest. In this study, we evaluated and compared the anti\u2010angiogenic effects and phenolic compound contents between wild (WOEs) and cultivated\n                      <jats:italic>O. japonicus<\/jats:italic>\n                      extracts (COEs) prepared under various extract conditions. WOEs and COEs suppressed cell proliferation of human umbilical vein endothelial cells (HUVECs) and inhibited vascular endothelial growth factor\u2010induced chemotactic migration, invasion, and capillary\u2010like tube formation in HUVECs. Among COEs, that prepared by 70% EtOH (70% CE) showed the most effective anti\u2010angiogenic activity in HUVECs. When compared to WOEs, total polyphenol and total flavonoid contents were 1.28 to 4.38 times higher in COEs, and 70% CE contained the greatest flavonoid contents (28.28\u00a0\u00b1\u00a00.93 mg%), as well as the highest levels of major phenolic compounds including gallic acid (21.84 \u00b5g\/mL), epicatechin\u2010gallate (6.58 \u00b5g\/mL), kaempferol (6.32 \u00b5g\/mL), and quercetin (8.55 \u00b5g\/mL). Although further studies are required to identify the molecular mechanisms behind these anti\u2010angiogenic effects, 70% CE could be used as an herbal medicine, functional food ingredient, and potent angiogenesis inhibitor.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Application<\/jats:title>\n                    <jats:p>\n                      Environmental factors such as altitude, nutrients, exposure to sunlight, and temperature can influence the type and quantity of bioactive components in plants. The advantage of cultivated plants is that the above\u2010mentioned factors can be artificially adjusted compared to wild plants. Based on economic efficiency, productivity, and consistent quality, anti\u2010angiogenesis activity of cultivated\n                      <jats:italic>O. japonicus<\/jats:italic>\n                      is of greater commercial value as a functional food than wild\n                      <jats:italic>O. japonicus<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.14675","type":"journal-article","created":{"date-parts":[[2019,6,20]],"date-time":"2019-06-20T02:54:30Z","timestamp":1560999270000},"page":"1764-1775","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Studies on the Anti\u2010Angiogenic Activities of Wild and Cultivated\n                    <i>Orostachys japonicus<\/i>\n                    Extracts in Human Umbilical Vein Endothelial Cells"],"prefix":"10.1111","volume":"84","author":[{"given":"Hyun\u2010Dong","family":"Cho","sequence":"first","affiliation":[{"name":"Industry\u2010Academy Cooperation Dong\u2010A Univ.  Busan 49315 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwan\u2010Woo","family":"Lee","sequence":"additional","affiliation":[{"name":"Dept. of Biotechnology Dong\u2010A Univ.  Busan 49315 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeong\u2010Seon","family":"Won","sequence":"additional","affiliation":[{"name":"Dept. of Biotechnology Dong\u2010A Univ.  Busan 49315 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong\u2010Young","family":"Shin","sequence":"additional","affiliation":[{"name":"Dept. of Development in Oriental Medicine Resources Sunchon Natl. Univ.  Suncheon 57922 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2391-9146","authenticated-orcid":false,"given":"Kwon\u2010Il","family":"Seo","sequence":"additional","affiliation":[{"name":"Dept. of Biotechnology Dong\u2010A Univ.  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