{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:34:31Z","timestamp":1790220871618,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T00:00:00Z","timestamp":1569196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      This study provides phenotypic and molecular analyses of the antibiotic resistance of\n                      <jats:italic>Ensifer adhaerens<\/jats:italic>\n                      strain YX1 (CICC 11008s), a strain that was identified using a polyphasic taxonomy approach. The antibiotic resistance profile of\n                      <jats:italic>E. adhaerens<\/jats:italic>\n                      YX1 was assessed using the Clinical &amp; Laboratory Standards Inst. (CLSI) method. The strain was susceptible to ciprofloxacin, levofloxacin, norfloxacin, ofloxacin, gentamicin, tobramycin, chloramphenicol, tetracycline, imipenem, and ceftazidime, and resistant to kanamycin, streptomycin, fosfomycin, and nitrofurantoin. The antibiotic resistance genes\n                      <jats:italic>nsfA<\/jats:italic>\n                      ,\n                      <jats:italic>nsfB<\/jats:italic>\n                      ,\n                      <jats:italic>fosA<\/jats:italic>\n                      ,\n                      <jats:italic>aph<\/jats:italic>\n                      , and\n                      <jats:italic>aadA1<\/jats:italic>\n                      were not detected in\n                      <jats:italic>E. adhaerens<\/jats:italic>\n                      YX1 via PCR using gene\u2010specific primers. Subsequently, the genome sequence of\n                      <jats:italic>E. adhaerens<\/jats:italic>\n                      was screened for antibiotic genes. Although no antibiotic resistance genes were identified using the ResFinder database, five genes copies of one resistance gene,\n                      <jats:italic>adeF<\/jats:italic>\n                      , were detected using the Comprehensive Antibiotic Resistance Database (CARD). The results of this study will be useful for understanding the phenotypic and genotypic aspects of\n                      <jats:italic>E. adhaerens<\/jats:italic>\n                      antibiotic resistance. No safety issues were identified for\n                      <jats:italic>E. adhaerens<\/jats:italic>\n                      YX1 in terms of antibiotic resistance. Performing similar studies will be conducive to the safety assessment and control of the use of\n                      <jats:italic>E. adhaerens<\/jats:italic>\n                      in the food and feed industry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Application<\/jats:title>\n                    <jats:p>\n                      Few relevant reports are currently available regarding antibiotic resistance assessments or other safety evaluations for\n                      <jats:italic>Ensifer adhaerens<\/jats:italic>\n                      . Because of a lack of relevant information on the safety of this bacterium, including the genetic basis of antibiotic resistance in the production strain, it has not been recommended for use in the \u201cqualified presumption of safety\u201d (QPS) list and subsequent updated lists. The current study shows no safety issue of\n                      <jats:italic>E. adhaerens<\/jats:italic>\n                      YX1 in terms of its antibiotic resistance. These results are important as they provide an initial basis for an understanding of the antibiotic resistance\/susceptibility of\n                      <jats:italic>E. adhaerens<\/jats:italic>\n                      YX1 (CICC 11008s), which produces vitamin B\n                      <jats:sub>12<\/jats:sub>\n                      and is widely used in the food and feed industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.14804","type":"journal-article","created":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T19:59:15Z","timestamp":1569268755000},"page":"2925-2931","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification and Antibiotic Resistance Assessment of\n                    <i>Ensifer adhaerens<\/i>\n                    YX1, a Vitamin B\n                    <sub>12<\/sub>\n                    \u2010Producing Strain Used as a Food and Feed Additive"],"prefix":"10.1111","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3741-7443","authenticated-orcid":false,"given":"Ting","family":"Zhao","sequence":"first","affiliation":[{"name":"China National Research Inst. of Food &amp; Fermentation Industries Co., Ltd China Center of Industrial Culture Collection  Building 6, No. 24 Yard, Jiuxianqiao Middle Road, Chaoyang District Beijing 100015 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Cheng","sequence":"additional","affiliation":[{"name":"China National Research Inst. of Food &amp; Fermentation Industries Co., Ltd China Center of Industrial Culture Collection  Building 6, No. 24 Yard, Jiuxianqiao Middle Road, Chaoyang District Beijing 100015 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010hua","family":"Cao","sequence":"additional","affiliation":[{"name":"China National Research Inst. of Food &amp; Fermentation Industries Co., Ltd China Center of Industrial Culture Collection  Building 6, No. 24 Yard, Jiuxianqiao Middle Road, Chaoyang District Beijing 100015 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur C.","family":"Ouwehand","sequence":"additional","affiliation":[{"name":"DuPont Nutrition &amp; Health  Sokeritehtaantie 20 02460 Kantvik Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cui\u2010feng","family":"Jiao","sequence":"additional","affiliation":[{"name":"Hebei Yuxing Bio\u2010engineering Co., Ltd.  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