{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T23:33:20Z","timestamp":1783035200366,"version":"3.54.6"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T00:00:00Z","timestamp":1585267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004480","name":"Natural Science Foundation of Shanxi Province","doi-asserted-by":"publisher","award":["201901D111287"],"award-info":[{"award-number":["201901D111287"]}],"id":[{"id":"10.13039\/501100004480","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Naked oat globulin was hydrolyzed by alcalase, flavourzyme, pepsin, and trypsin in sequence. The hydrolysates (NOGH) were purified using gel chromatography, reversed\u2010phase high performance liquid chromatography (RP\u2010HPLC). Finally, fraction D7d with the highest ACE\u2010inhibitory was subjected to liquid chromatography\u2010mass spectrometry analysis and 14 peptides were identified. Of which, peptide SSYYPFK (890.4\u00a0Da) was chose to synthesize based on\n                      <jats:italic>in silico<\/jats:italic>\n                      analysis. The SSYYPFK demonstrated high ACE\u2010inhibitory activity (IC\n                      <jats:sub>50<\/jats:sub>\n                      : 91.82\u00a0\u00b5M) with competitive inhibition mode, and could effectively (\n                      <jats:italic>P &lt;\u00a0<\/jats:italic>\n                      0.05) lower the systolic blood pressure and diastolic pressure of spontaneously hypertensive rats at the concentration of 100 to 150\u00a0mg\/kg body weight. Molecular docking simulation demonstrated that SSYYPFK could bind with the active site S1 of ACE via short hydrogen bonds. It could remain the ACE\u2010inhibitory activity after simulated gastrointestinal hydrolysis. Moreover, SSYYPFK showed acceptable renin and endothelin\u20101 suppressing capacity (47.59% and 27.88% at 1.5\u00a0mg\/mL, respectively). These results indicated that SSYYPFK may have similar antihypertensive mechanism with captopril, and could be develop to natural antihypertensive products.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Application<\/jats:title>\n                    <jats:p>\n                      One novel ACE\u2010inhibitory peptide SSYYPFK (890.4\u00a0Da) was identified from naked oat globulin hydrolysates. It exhibited relatively high renin and intracellular endothelin\u20101 suppressing capacity, and could effectively (\n                      <jats:italic>P<\/jats:italic>\n                      &lt;\u00a00.05) lower the systolic blood pressure and diastolic pressure of spontaneously hypertensive rats. This peptide could be used as natural and safe nutraceuticals and\/or functional ingredients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.15115","type":"journal-article","created":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T12:31:36Z","timestamp":1585312296000},"page":"1328-1337","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Isolation of novel ACE\u2010inhibitory peptide from naked oat globulin hydrolysates\n                    <i>in silico<\/i>\n                    approach: Molecular docking,\n                    <i>in vivo<\/i>\n                    antihypertension and effects on renin and intracellular endothelin\u20101"],"prefix":"10.1111","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5482-0595","authenticated-orcid":false,"given":"Yajun","family":"Zheng","sequence":"first","affiliation":[{"name":"College of Food Science of Shanxi 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