{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:55:59Z","timestamp":1791420959758,"version":"4.3.3"},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T00:00:00Z","timestamp":1600819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Rapid and sensitive detection of live\/infectious foodborne pathogens is urgently needed in order to prevent outbreaks and food recalls. This study aimed to (1) evaluate the incorporation of propidium monoazide (PMA) into PCR or LAMP assays to selectively detect viable\n                    <jats:italic>Salmonella<\/jats:italic>\n                    Enteritidis following sublethal heat or UV treatment, and autoclave sterilization; and (2) compare the detection of PMA\u2010PCR and PMA\u2010LAMP to DNA\u2010based PCR and LAMP (without PMA), RNA\u2010based RT\u2010PCR and RT\u2010LAMP, and culture\u2010based methods. Nucleic acids (DNA or RNA) from 1\u2010mL\n                    <jats:italic>S<\/jats:italic>\n                    . Enteritidis samples were used for PCR, RT\u2010PCR, LAMP, and RT\u2010LAMP assays. Serially diluted samples were plated on Xylose Lysine Tergitol\u20104 agar for cultural enumeration. Comparable detection of overnight cultured\n                    <jats:italic>S<\/jats:italic>\n                    . Enteritidis was obtained by PMA\u2010PCR, PCR, and RT\u2010PCR, though 1 to 2 log less sensitive than cultural assays. PMA\u2010LAMP and RT\u2010LAMP showed similar detection of overnight cultures, being 1 to 2 log less sensitive than the LAMP assay, and \u223c4 log less than culture\u2010based detection. Autoclaved\n                    <jats:italic>S<\/jats:italic>\n                    . Enteritidis did not test positive by RNA\u2010based methods or PMA\u2010PCR, but PMA\u2010LAMP showed detection of 1 log CFU\/mL. PMA\u2010PCR and RT\u2010PCR showed comparable detection of sublethal heat\u2010treated cells to cultural assays, while PMA\u2010LAMP showed 1 to 2 log less detection. Our results suggest that PMA\u2010PCR and PMA\u2010LAMP assays are not suitable for selective viable cell detection after UV treatment. While PMA\u2010LAMP assay needs optimization, PMA\u2010PCR shows promise for live\/viable\n                    <jats:italic>S<\/jats:italic>\n                    . Enteritidis detection. PMA\u2010PCR shows potential for routine testing in the food industry with results within 1\u2010day, albeit depending on the inactivation method employed.\n                  <\/jats:p>","DOI":"10.1111\/1750-3841.15459","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T03:53:59Z","timestamp":1600833239000},"page":"3509-3516","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Propidium monoazide for viable\n                    <i>Salmonella enterica<\/i>\n                    detection by PCR and LAMP assays in comparison to RNA\u2010based RT\u2010PCR, RT\u2010LAMP, and culture\u2010based assays"],"prefix":"10.1111","volume":"85","author":[{"given":"Chayapa","family":"Techathuvanan","sequence":"first","affiliation":[{"name":"Department of Food Science University of Tennessee  2605 River Drive Knoxville TN 37996\u20104591 U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7388-4674","authenticated-orcid":false,"given":"Doris Helen","family":"D'Souza","sequence":"additional","affiliation":[{"name":"Department of Food Science University of Tennessee  2605 River Drive Knoxville TN 37996\u20104591 U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.1.420-425.2001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02137-09"},{"key":"e_1_2_7_4_1","unstructured":"Centers for Disease Control and Prevention (CDC). 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