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Marine food products such as\n                      <jats:italic>Octopus vulgaris<\/jats:italic>\n                      ink (OI) could be sources of compounds addressing these concerns. This study aimed to evaluate the antimutagenic, cytoprotective, antiproliferative, proapoptotic, and antioxidant capacity of OI extracts on human cancer cell lines (22Rv1, HeLa, A549). The ARPE\u201019 cell line was used as a reference human cell line to evaluate the ink's cytotoxicity. The water extract exhibited the highest antimutagenic and cytoprotective effect, but the dichloromethane extract (DM) showed the lowest half lethal concentration against 22Rv1 cells. Structural elucidation of purified DM fractions (F1, F2, F3) identified an unreported compound, N\u2010(2\u2010ozoazepan\u20103\u2010yl)\u2010pyrrolidine\u20102\u2010carboxamide (OPC). DM\u2010F2 showed high antiproliferative effect (LC\n                      <jats:sub>50<\/jats:sub>\n                      = 27.6 \u00b5g\/mL), reactive species modulation, early\u2010apoptosis induction (42.9%), and nuclei disruption in 22Rv1 cells.\n                      <jats:italic>In silico<\/jats:italic>\n                      analysis predicted high OPC affinity with Cyclin D1 (\u22126.70\u00a0kcal\/mol), suggesting its potential impact on cell cycle arrest. These results highlight the antimutagenic, cytoprotective, and antiproliferative potential health benefits derived from underutilized marine food products such as OI. Further investigations at\n                      <jats:italic>in vitro<\/jats:italic>\n                      or\n                      <jats:italic>in vivo<\/jats:italic>\n                      levels are required to elucidate mechanisms and health benefits from OI.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Application<\/jats:title>\n                    <jats:p>\n                      <jats:italic>O. vulgaris<\/jats:italic>\n                      ink is an underutilized marine natural product that could be a source of biological compounds with potential health benefits such as antioxidant activity and cancer prevention.\n                    <\/jats:p>\n                  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