{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:10:16Z","timestamp":1790295016891,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,5,30]],"date-time":"2021-05-30T00:00:00Z","timestamp":1622332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Hydrolysates containing angiotensin I\u2010converting enzyme (ACE)\u2010inhibitory peptide were prepared from protein of Alaska pollack skins using alcalase and trypsin. The protein hydrolysate was separated by ultrafiltration, Sephadex G\u201025 gel filtration chromatography and reversed phase high\u2010performance liquid chromatography (HPLC), from which a novel purified peptide was obtained. Both random coil structure and \u03b2\u2010sheet in the purified peptide were revealed in Fourier transform infrared spectrum. The amino sequence of the purified peptide was identified as GPLGVP, VLYPVK, VFLENVLR, and FEEF by HPLC\u2010Q\u2010TOF\u2010MS (HPLC\u2010quadrupole time\u2010of\u2010flight mass spectrometry). The peptide GPLGVP whose molecular weight was 538.31\u00a0Da showed the highest ACE inhibitory activity (IC\n                      <jats:sub>50\u00a0<\/jats:sub>\n                      =\u00a0105.8 \u00b5M). The purified peptide featured a noncompetitive inhibition kinetic mechanism was shown in the Lineweaver\u2013Burk plots and was susceptible to enzymes as indicated in the studies on stability of gastrointestinal proteases. Moreover, the peptide GPLGVP can combine ACE catalytic pocket through hydrogen bonds and other forces with high binding power as disclosed in molecular docking simulation, which provides the inhibitory effect of GPLGVP on ACE.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.15754","type":"journal-article","created":{"date-parts":[[2021,5,30]],"date-time":"2021-05-30T23:01:18Z","timestamp":1622415678000},"page":"2457-2467","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Purification and characterization of a novel angiotensin I\u2010converting enzyme\u2010inhibitory peptide derived from Alaska pollack skins"],"prefix":"10.1111","volume":"86","author":[{"given":"Guilan","family":"Yang","sequence":"first","affiliation":[{"name":"Yantai Institute of Coastal Zone Research Chinese Academy of Sciences  Yantai PR China"},{"name":"School of Pharmacy Binzhou Medical University  Yantai PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Qin","sequence":"additional","affiliation":[{"name":"Yantai Institute of Coastal Zone Research Chinese Academy of Sciences  Yantai PR China"},{"name":"Center for Ocean Mega\u2010Science Chinese Academy of Sciences  Qingdao PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4017-2520","authenticated-orcid":false,"given":"Wenjun","family":"Li","sequence":"additional","affiliation":[{"name":"Yantai Institute of Coastal Zone Research Chinese Academy of Sciences  Yantai PR China"},{"name":"Center for Ocean Mega\u2010Science Chinese Academy of Sciences  Qingdao PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2019.04.002"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.06.112"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2013.03.091"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0032-9592(00)00297-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.10041"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2011.11.006"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9040316"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/md16090299"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.17582\/journal.pjz\/20190416040458"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.03.047"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1965.tb02091.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2015.10.025"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.4037"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.12031"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2999-3_5"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ifset.2016.03.014"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2015.11.020"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2016.01.009"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2006.01.057"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2009.04.086"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11061351"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9FO01980D"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2016.07.006"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0RA00093K"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.05.087"},{"key":"e_1_2_8_27_1","unstructured":"PRC N. H. A. F.(2015).Report on Chinese residents\u2019 chronic diseases and nutrition."},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2018.11.017"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2017.09.006"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2020.02.010"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.02.107"},{"key":"e_1_2_8_32_1","unstructured":"World Health Organization (WHO). (2018).Global health observatory data: Raised blood pressure situation and trends."},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2018.12.018"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf8028557"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.01.032"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8FO00569A"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.127921"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2016.11.021"}],"container-title":["Journal of Food Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.15754","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1750-3841.15754","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.15754","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T19:44:03Z","timestamp":1762199043000},"score":1,"resource":{"primary":{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/10.1111\/1750-3841.15754"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,30]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1111\/1750-3841.15754"],"URL":"https:\/\/doi.org\/10.1111\/1750-3841.15754","archive":["Portico"],"relation":{},"ISSN":["0022-1147","1750-3841"],"issn-type":[{"value":"0022-1147","type":"print"},{"value":"1750-3841","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,30]]},"assertion":[{"value":"2021-01-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}