{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T07:51:10Z","timestamp":1788162670900,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,6,19]],"date-time":"2021-06-19T00:00:00Z","timestamp":1624060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Ribavirin (RBV) is an effective antiviral drug, whose use is prohibited in animal husbandry worldwide. In this work, a novel immunizing hapten of RBV, named Hapten 4, was designed by comparing the conformational and electronic properties of RBV and haptens based on computational chemistry. Hapten 4 was synthesized and conjugated with carrier proteins to produce monoclonal antibody (mAb). The obtained mAb 4C3 for RBV exhibited an IC\n                      <jats:sub>50<\/jats:sub>\n                      value of 6.24\u00a0ng\/ml in an indirect competitive enzyme\u2010linked immunosorbent assay (icELISA) and displayed no cross\u2010reaction with five other antiviral drugs, including amantadine. The applicability of the developed icELISA was verified in chicken, with a calculated limit of detection of 4.23\u00a0\u00b5g\/kg. The recoveries in spiked chicken were 79.2%\u2013107.3% with a coefficient of variation less than 15.9%. The results indicated that the produced antibody from the new hapten was reliable and would be useful for RBV screening in chicken.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Application<\/jats:title>\n                    <jats:p>RBV is a broad\u2010spectrum antiviral drug, which is commonly used illegally in poultry farms. A high\u2010affinity mAb 4C3 against RBV was produced and used to develop icELISA with acceptable sensitivity and accuracy. The constructed icELISA has excellent performance for detecting RBV residues in chicken.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.15789","type":"journal-article","created":{"date-parts":[[2021,6,19]],"date-time":"2021-06-19T15:01:16Z","timestamp":1624114876000},"page":"2851-2860","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Synthesis of hapten, production of monoclonal antibody, and development of immunoassay for ribavirin detection in chicken"],"prefix":"10.1111","volume":"86","author":[{"given":"Jianyu","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Beijing Key Laboratory of Detection Technology for Animal\u2010Derived Food Safety Beijing Laboratory for Food Quality and Safety China Agricultural University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Beijing Key Laboratory of Detection Technology for Animal\u2010Derived Food Safety Beijing Laboratory for Food Quality and Safety China Agricultural University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuezhi","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Beijing Key Laboratory of Detection Technology for Animal\u2010Derived Food Safety Beijing Laboratory for Food Quality and Safety China Agricultural University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiya","family":"Zhang","sequence":"additional","affiliation":[{"name":"Henan Province Engineering Research Center for Food Safety Control of Processing and Circulation, College of Food Science and Technology Henan Agricultural University  Zhengzhou Henan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongfang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Beijing Key Laboratory of Detection Technology for Animal\u2010Derived Food Safety Beijing Laboratory for Food Quality and Safety China Agricultural University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9553-259X","authenticated-orcid":false,"given":"Kai","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Beijing Key Laboratory of Detection Technology for Animal\u2010Derived Food Safety Beijing Laboratory for Food Quality and Safety China Agricultural University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuebin","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Genetic Toxicology Shenzhen Center for Disease Control and Prevention  People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suxia","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Beijing Key Laboratory of Detection Technology for Animal\u2010Derived Food Safety Beijing Laboratory for Food Quality and Safety China Agricultural University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0167-9559","authenticated-orcid":false,"given":"Zhanhui","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Beijing Key Laboratory of Detection Technology for Animal\u2010Derived Food Safety Beijing Laboratory for Food Quality and Safety China Agricultural University  Beijing People's Republic of 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