{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:43:43Z","timestamp":1787161423176,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T00:00:00Z","timestamp":1648425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2022,5]]},"abstract":"\n Abstract<\/jats:title>\n \n Bifidobacterium animalis<\/jats:italic>\n CP\u20109 was a commensal strain isolated from human breast milk. In this study, genetic and 90\u2010day oral toxicity were assessed in rodents for its safety. Ames test as well as in vivo bone marrow micronucleus and spermatocyte chromosomal aberration were surveyed in mice.\n B. animalis<\/jats:italic>\n CP\u20109 exhibited no mutagenic activity in the Ames test at the highest tested dosage (5000\u00a0\u00b5g\/plate) with or without metabolic activation. No evidence of in vivo genetic toxicity was observed at the maximum tested dosage of 10\u00a0g\/kg body weight (BW). Furthermore, there was no statistically significant difference of the biochemical and histological parameters in the rats administrated with\n B. animalis<\/jats:italic>\n CP\u20109 at dosages of 0, 0.25, 0.5, or 1.5\u00a0g\/kg BW\/day. No indication of concern for pathogenicity was exhibited during evaluation of\n Bifidobacterium<\/jats:italic>\n ssp. generally, or\n B. animalis<\/jats:italic>\n specifically. It was noted that\n B. animalis<\/jats:italic>\n CP\u20109 was able to survive in gastric acid\u2010like and high bile salt environment, and showed strong adhesion to the intestinal epithelial cells, Caco\u20102. Intriguingly,\n B. animalis<\/jats:italic>\n CP\u20109 decreased olic acid\u2010induced triglyceral (TG) accumulation in the Caco\u20102 cells, and viable\n B. animalis<\/jats:italic>\n CP\u20109 had a better bacteriostatic activity compared to another well\u2010documented\n B. animalis<\/jats:italic>\n ssp.\n lactis<\/jats:italic>\n , BB\u201012. Based on the present study,\n B. animalis<\/jats:italic>\n CP\u20109 can be a safe probiotic supplement and may improve the health of host.\n <\/jats:p>\n <\/jats:sec>\n \n Practical Application<\/jats:title>\n \n Although the health benefits of probiotics are well known, the safety of a probiotic product is acquired particularly for a long\u2010term consumption. We conduct the safety of\n B. animalis<\/jats:italic>\n CP\u20109 isolated from human breast milk, and demonstrate no toxicity concern in vitro and in vivo. Hence,\n B. animalis<\/jats:italic>\n CP\u20109 powder can be used as a commercial and safe probiotic supplement with some health benefits.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/1750-3841.16129","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T00:37:55Z","timestamp":1648514275000},"page":"2211-2228","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Safety and the probiotic potential of\n Bifidobacterium animalis<\/i>\n CP\u20109"],"prefix":"10.1111","volume":"87","author":[{"given":"Hui\u2010Yun","family":"Tsai","sequence":"first","affiliation":[{"name":"Department of Nutrition and Health Science Fooyin University Kaohsiung Taiwan"},{"name":"Aging and Disease Prevention Research Center Fooyin University Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2989-3362","authenticated-orcid":false,"given":"Ya\u2010Chin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences and Biotechnology Fooyin University Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chorng\u2010An","family":"Liao","sequence":"additional","affiliation":[{"name":"Aging and Disease Prevention Research Center Fooyin University Kaohsiung Taiwan"},{"name":"Biomed Analysis Center Fooyin University Hospital Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Yan","family":"Su","sequence":"additional","affiliation":[{"name":"Aging and Disease Prevention Research Center Fooyin University Kaohsiung Taiwan"},{"name":"School of Pharmacy Kaohsiung Medical University Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7567-2897","authenticated-orcid":false,"given":"Cheng\u2010Hsieh","family":"Huang","sequence":"additional","affiliation":[{"name":"Aging and Disease Prevention Research Center Fooyin University Kaohsiung Taiwan"},{"name":"Ph. 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Program in Environmental and Occupational Medicine College of Medicine, Kaohsiung Medical University and National Health Research Institutes Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Hsi","family":"Chiu","sequence":"additional","affiliation":[{"name":"Aging and Disease Prevention Research Center Fooyin University Kaohsiung Taiwan"},{"name":"Department of Medical Laboratory Sciences and Biotechnology Fooyin University Kaohsiung Taiwan"},{"name":"Biomed Analysis Center Fooyin University Hospital Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7780-7983","authenticated-orcid":false,"given":"Yao\u2010Tsung","family":"Yeh","sequence":"additional","affiliation":[{"name":"Aging and Disease Prevention Research Center Fooyin University Kaohsiung Taiwan"},{"name":"Department of Medical Laboratory Sciences and Biotechnology Fooyin University Kaohsiung Taiwan"},{"name":"Biomed Analysis Center Fooyin University Hospital Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(98)00198-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635589709514581"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407076101"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/026119290503300602"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.03.009"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/368080"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01430-09"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/lam.13275"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/254239"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2021.04.006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.5.1209"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1086\/497832"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-08051-7"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.106.3.824-829.1971"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-019-02598-0"},{"key":"e_1_2_8_17_1","unstructured":"Food Safety National Standard (China)Protocol GB15193.4\u20102014 Bacterial Reverse Mutation Test is identical to the Organisation for Economic Co\u2010operation and Development (OECD) Test Guideline 471 (OECD 1997)."},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-011-2364-8_1"},{"key":"e_1_2_8_19_1","unstructured":"G. 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