{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:08:33Z","timestamp":1790780913038,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T00:00:00Z","timestamp":1698364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2023,12]]},"abstract":"\n Abstract<\/jats:title>\n \n Secoisolariciresinol diglucoside (\n SDG<\/jats:bold>\n ) and tracheloside (\n TCL<\/jats:bold>\n ) are the main lignan components of flaxseed cake and safflower seed cake, which are by\u2010products of oil extraction. Both\n SDG<\/jats:bold>\n and\n TCL<\/jats:bold>\n are metabolized into mammalian lignan enterolactone (\n EL<\/jats:bold>\n ) with the involvement of intestinal bacteria. In this research, we evaluated the anti\u2010osteoporosis effects of\n SDG<\/jats:bold>\n and the in vivo metabolites\n EL<\/jats:bold>\n and enterodiol (\n ED<\/jats:bold>\n ) prepared in our previous work, as well as the newly isolated chemical constituents from safflower seed, including\n TCL<\/jats:bold>\n , the lactone ring opening product of TCL (\n OTCL<\/jats:bold>\n ) and two alkaloids on the alloxan\u2010induced zebrafish model. All the compounds showed significant anti\u2010osteoporosis effects at 80\u00a0\u00b5M, with\n p<\/jats:italic>\n \u00a0<\u00a00.05 for\n EL<\/jats:bold>\n and\n p<\/jats:italic>\n \u00a0<\u00a00.001 for other compounds compared with the model.\n SDG<\/jats:bold>\n and\n TCL<\/jats:bold>\n showed the most significant and concentration\u2010dependent effects, with\n p<\/jats:italic>\n \u00a0<\u00a00.001 compared with model at 20\u00a0\u00b5M. The alkaloids,\n N<\/jats:italic>\n \u2010coumaroylserotonin glucoside and\n N<\/jats:italic>\n \u2010feruloylserotonin glucoside, also showed anti\u2010osteoporosis at 20\u00a0\u00b5M with\n p<\/jats:italic>\n \u00a0<\u00a00.01, whereas\n EL<\/jats:bold>\n ,\n ED<\/jats:bold>\n , and\n OTCL<\/jats:bold>\n showed no significant effects. Quantitative real\u2010time polymerase chain reaction revealed that\n SDG<\/jats:bold>\n and\n TCL<\/jats:bold>\n upregulated the expression of osteogenic genes\n Runx2, SP7, OPG, Col1a1a, Alp, ON, OPN<\/jats:italic>\n , and\n OCN<\/jats:italic>\n in alloxan\u2010treated zebrafish. The in vivo metabolite of lignans,\n EL<\/jats:bold>\n , showed significant anti\u2010inflammatory effect (\n p<\/jats:italic>\n \u00a0<\u00a00.01) at 20\u00a0\u00b5M, which might also help to combat osteoporosis and other complications caused by excessive immune response in the body. The results provided scientific data for using the oil extraction by\u2010products as sources of anti\u2010osteoporosis compounds.\n <\/jats:p>\n <\/jats:sec>\n \n Practical Application<\/jats:title>\n This study found that lignans in flaxseed cake and safflower seed cake exhibited anti\u2010osteoporosis effects by upregulating the expression of osteogenic genes, making the oil extraction by\u2010products sources of anti\u2010osteoporosis compounds.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/1750-3841.16816","type":"journal-article","created":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T09:22:41Z","timestamp":1698398561000},"page":"5278-5290","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Anti\u2010osteoporosis effects of mammalian lignans and their precursors from flaxseed and safflower seed using zebrafish model"],"prefix":"10.1111","volume":"88","author":[{"given":"Xin\u2010Yue","family":"Yang","sequence":"first","affiliation":[{"name":"School of Life Sciences Inner Mongolia University Hohhot P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan\u2010Dan","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life Sciences Inner Mongolia University Hohhot P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong\u2010Cong","family":"Zhuang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Inner Mongolia University Hohhot P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1189-7901","authenticated-orcid":false,"given":"Chao\u2010Mei","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Life Sciences Inner Mongolia University Hohhot P. R. 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