{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T03:27:39Z","timestamp":1785900459985,"version":"3.56.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T00:00:00Z","timestamp":1703116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972167"],"award-info":[{"award-number":["31972167"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFC1603800"],"award-info":[{"award-number":["2018YFC1603800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Cronobacter sakazakii<\/jats:italic>\n                    , an important Gram\u2010negative foodborne pathogen, can cause neonatal meningitis and sepsis with high rates of infection and death. Gene\n                    <jats:italic>ESA_RS15745<\/jats:italic>\n                    encodes a sugar transporter protein, which is not only essential for osmotic pressure maintenance during bacterial growth and reproduction but also associated with their desiccation tolerance, motility, and biofilm formation. Here, a mutant strain of\n                    <jats:italic>ESA_RS15745<\/jats:italic>\n                    (\n                    <jats:italic>\u0394ESA_RS15745<\/jats:italic>\n                    ) and the complementation strain (\n                    <jats:italic>cpESA_RS15745<\/jats:italic>\n                    ) were constructed using a suicide vector knockout and gene complementation.\n                    <jats:italic>\u0394ESA_RS15745<\/jats:italic>\n                    was found to have a decrease in its ability to transport maltose and trehalose and resist desiccation, whereas an increase in the ability of motility and biofilm formation, implying that\n                    <jats:italic>ESA_RS15745<\/jats:italic>\n                    may positively regulate sugar transport and desiccation tolerance and negatively regulate motility and biofilm formation. To further investigate the molecular mechanisms underlying the function of related genes, RNA\u2010seq was performed to explore the differentially expressed genes in the mutants. RNA\u2010seq results showed the upregulation of 114 genes (mainly including those regulating chemotaxis and flagellar motility) and the downregulation of 22 genes (mainly including those regulating sugar transport). qRT\u2010PCR analysis supported the RNA\u2010seq results and showed that\n                    <jats:italic>ESA_RS15745<\/jats:italic>\n                    may influence the dehydration tolerance though decreasing the intracellular trehalose content and negatively regulate the motility though the chemotactic signaling pathway. In addition, the biofilm formation of\n                    <jats:italic>C. sakazakii<\/jats:italic>\n                    should also be speculated to negatively regulate by\n                    <jats:italic>ESA_RS15745<\/jats:italic>\n                    by consuming the extracellular carbohydrates concentration and then downregulating the intracellular cyclic diguanosine monophosphate. This study offers a reference for comprehending the molecular mechanism of gene\n                    <jats:italic>ESA_RS15745<\/jats:italic>\n                    in\n                    <jats:italic>C. sakazakii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1750-3841.16872","type":"journal-article","created":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T04:03:35Z","timestamp":1703131415000},"page":"581-595","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects and molecular mechanism of sugar transporter\n                    <i>ESA_RS15745<\/i>\n                    on desiccation resistance, motility, and biofilm formation of\n                    <i>Cronobacter sakazakii<\/i>"],"prefix":"10.1111","volume":"89","author":[{"given":"Li","family":"Ping","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering Tianjin University of Science and Technology  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5172-1575","authenticated-orcid":false,"given":"Zhang","family":"Zhengyang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering Tianjin University of Science and Technology  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si","family":"Mohan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering Tianjin University of Science and Technology  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Ruihong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering Tianjin University of Science and 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