{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T13:32:50Z","timestamp":1789651970959,"version":"4.0.0"},"reference-count":57,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      In the study, papain was used to hydrolyze tilapia (\n                      <jats:italic>Oreochromis mossambicus<\/jats:italic>\n                      ) skin to obtain a tilapia skin hydrolysate (TSH) with dual angiotensin\u2010converting enzyme (ACE) and dipeptidyl peptidase IV (DPP\u2010IV) inhibitory activities. The resulting TSH was sequentially fractionated by ultrafiltration, size exclusion separation chromatography, and reverse\u2010phase high\u2010performance liquid chromatography. Its inhibitory effects on ACE and DPP\u2010IV were determined by commercial reagent kits. Two peptides purified from TSH were identified as Gly\u2010Pro\u2010Leu\u2010Gly\u2010Ala\u2010Leu (GPLGAL) and Lys\u2010Pro\u2010Ala\u2010Gly\u2010Asn (KPAGN) by the ultra\u2010performance liquid chromatography\u2013tandem mass spectrometry (UPLC\u2013MS\/MS). Inhibitory concentration (IC\n                      <jats:sub>50<\/jats:sub>\n                      ) of GPLGAL on ACE and DPP\u2010IV were 117.20\u00a0\u00b1\u00a01.69 and 187.10\u00a0\u00b1\u00a02.75\u00a0\u00b5M, respectively. IC\n                      <jats:sub>50<\/jats:sub>\n                      of KPAGN on ACE and DPP\u2010IV were 137.40\u00a0\u00b1\u00a02.33 and 259.20\u00a0\u00b1\u00a02.85\u00a0\u00b5M, respectively. The molecular simulation demonstrated that the binding affinities of GPLGAL to ACE and DPP\u2010IV proteins were \u22128.5 and \u22127.4\u00a0kcal\/mol, respectively, whereas those of KPAGN to ACE and DPP\u2010IV proteins were \u22127.9 and \u22126.7\u00a0kcal\/mol, respectively. GPLGAL interacted with 21 amino acid residues of the ACE active site, whereas KPAGN engaged with 19 amino acid residues. Additionally, GPLGAL interacted with 10 amino acid residues of the DPP\u2010IV active site, whereas KPAGN engaged with 13 amino acid residues. The two peptides predominantly occupied the active sites of ACE (His513, Tyr523, and Ala354) and DPP\u2010IV (Tyr662 and Arg125) through hydrogen bonding. This leads to the deactivation of ACE and DPP\u2010IV.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Application<\/jats:title>\n                    <jats:p>Accelerate tilapia skin development and high\u2010value utilization; provide foundation for preparing the peptides with dual ACE and DPP\u2010IV inhibiting activity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.17059","type":"journal-article","created":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T02:30:32Z","timestamp":1713493832000},"page":"3603-3617","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Two novel angiotensin\u2010converting enzyme (ACE) and dipeptidyl peptidase IV (DPP\u2010IV) inhibiting peptides from tilapia (\n                    <i>Oreochromis mossambicus<\/i>\n                    ) skin and their molecular docking mechanism"],"prefix":"10.1111","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1485-1080","authenticated-orcid":false,"given":"Jiayi","family":"Chen","sequence":"first","affiliation":[{"name":"College of Food Science and Technology Guangdong Ocean University  Zhanjiang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwu","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Guangdong Ocean University  Zhanjiang P. R. China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety  Zhanjiang P. R. China"},{"name":"Guangdong Province Engineering Laboratory for Marine Biological Products  Zhanjiang P. R. China"},{"name":"Guangdong Provincial Engineering Technology Research Center of seafood  Zhanjiang P. R. China"},{"name":"Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution  Zhanjiang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Guangdong Ocean University  Zhanjiang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7030-6249","authenticated-orcid":false,"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Guangdong Ocean University  Zhanjiang P. R. China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety  Zhanjiang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shucheng","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Guangdong Ocean University  Zhanjiang P. R. China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety  Zhanjiang P. R. China"},{"name":"Guangdong Province Engineering Laboratory for Marine Biological Products  Zhanjiang P. R. China"},{"name":"Guangdong Provincial Engineering Technology Research Center of seafood  Zhanjiang P. R. China"},{"name":"Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution  Zhanjiang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2023.135249"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.12462"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2019.103455"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.981163"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/md17070431"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2015.10.070"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.2253"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2016.10.030"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S51325"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10499\u2010023\u201001117\u20104"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.11532"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41538\u2010018\u20100033\u20105"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/md21100516"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197\u2010020\u201004944\u2010y"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2016.10.019"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750\u20103841.13735"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2020.109161"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2022.115304"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.128719"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijfs.13939"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2016.05.008"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2017.07.026"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10010076"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12121866"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859\u2010018\u20102462\u20101"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2023.115254"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c08225"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01579"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11172576"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/d1fo01103k"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2014.12.028"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12082914"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13942"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2017.06.065"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11947\u2010019\u201002371\u20102"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.03.123"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41706"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2019.01.016"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.15835"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.868681"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12112216"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2021.4928"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.132089"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/md19120651"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2fo00275b"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.957778"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodhyd.2018.02.048"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41440\u2010023\u201001233\u2010x"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.08.032"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1590\/fst.106921"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.133821"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.138370"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2017.1325902"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2022.105151"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2020.109534"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2022.111176"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2023.102638"}],"container-title":["Journal of Food Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1750-3841.17059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T17:38:23Z","timestamp":1762191503000},"score":1,"resource":{"primary":{"URL":"https:\/\/ift.onlinelibrary.wiley.com\/doi\/10.1111\/1750-3841.17059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,18]]},"references-count":57,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1111\/1750-3841.17059"],"URL":"https:\/\/doi.org\/10.1111\/1750-3841.17059","archive":["Portico"],"relation":{},"ISSN":["0022-1147","1750-3841"],"issn-type":[{"value":"0022-1147","type":"print"},{"value":"1750-3841","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,18]]},"assertion":[{"value":"2023-09-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}