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The bioactive phytochemicals in Moringa seed extract (MSE) indicated large numbers of phytochemicals (21 compounds) with dominant abundance for cycloisolongifolene, 8,9\u2010dehydro\u20109\u2010vinyl, and chamazulene accounting for 12.7% and 12.19% of the total detected compounds. The MSE showed a potent anticancer effect toward Caco\u20102, MDA, and HepG\u20102 cells with half\u2010maximal inhibitory concentration (IC\n                    <jats:sub>50<\/jats:sub>\n                    ) values of 9.15\u00a0\u00b1\u00a01.18, 4.85\u00a0\u00b1\u00a00.11, and 7.36\u00a0\u00b1\u00a00.22\u00a0\u00b5g\/mL, respectively, with higher safety (\u226531\u2010folds) toward normal human cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    of 150.7\u00a0\u00b1\u00a011.11\u00a0\u00b5g\/mL). It appears that MSE stimulates selective\u2010dose\u2010dependent cell shrinkage, and nuclear condensation in the tumor cells, which finally induces the apoptosis pathway to increase its anticancer action. Additionally, MSE showed a potent capability to stimulate cell cycle arrest in both main checkpoint phases (G0\/G1 and G2\/M) of cell population growth. The apoptotic death stimulation was confirmed through upregulation of tumor protein p53 (p53) and cyclin\u2010dependent kinase inhibitor p21 (p21) expression by more than three\u2010 to sixfold and downregulation of B\u2010cell lymphoma 2 expression (threefold) in MSE\u2010treated cells compared to 5\u2010fluorouracil (5\u2010FU)\u2010treated tumor cells. Furthermore, the MSE revealed strong anti\u2010inflammatory activity with significant antioxidant activity by lowering nitric oxide levels and enhancing the superoxide dismutase activity. On the other hand, the MSE revealed broad\u2010spectrum antibacterial activity in a dose\u2010dependent manner against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    minimum inhibitory concentration (MIC of 1.25\u00a0mg\/mL), followed by\n                    <jats:italic>Salmonella typhimurium<\/jats:italic>\n                    (MIC of 1.23\u00a0mg\/mL), whereas\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    was the least sensitive to MSE activity (MIC of 22.5\u00a0mg\/mL) with significant antibiofilm activity against sensitive pathogens.\n                  <\/jats:p>","DOI":"10.1111\/1750-3841.17223","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T22:24:23Z","timestamp":1719959063000},"page":"5130-5149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<i>Moringa oleifera<\/i>\n                    seed methanol extract with consolidated antimicrobial, antioxidant, anti\u2010inflammatory, and anticancer activities"],"prefix":"10.1111","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0724-8477","authenticated-orcid":false,"given":"Esmail M.","family":"El\u2010Fakharany","sequence":"first","affiliation":[{"name":"Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI) City of Scientific Research and Technological Applications (SRTA\u2010City)  New Borg El\u2010Arab City Alexandria Egypt"},{"name":"Pharmaceutical and Fermentation Industries Development Center City of Scientific Research and Technological Applications (SRTA\u2010City)  New Borg El\u2010Arab City Alexandria Egypt"},{"name":"Pharos University in Alexandria  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wafaa B.","family":"Elsharkawy","sequence":"additional","affiliation":[{"name":"Physics Department, College of Science and Humanities Studies Prince Sattam bin Abdulaziz University  AlKharj Saudi\u00a0Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousra A.","family":"El\u2010Maradny","sequence":"additional","affiliation":[{"name":"Pharmaceutical and Fermentation Industries Development Center City of Scientific Research and Technological Applications (SRTA\u2010City)  New Borg El\u2010Arab City Alexandria Egypt"},{"name":"Microbiology and Immunology, Faculty of Pharmacy Arab Academy for Science, Technology and Maritime Transport (AASTMT)  El\u2010Alamein Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamada","family":"El\u2011Gendi","sequence":"additional","affiliation":[{"name":"Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute City of Scientific Research and Technological Applications (SRTA\u2010City)  New Borg El\u2011Arab City Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants11050594"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/agriculture8090145"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2022.102031"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2014.15.20.8571"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-30845-z"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph13080193"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2021.05.003"},{"key":"e_1_2_8_9_1","first-page":"243","article-title":"Analysis of bioactive compounds in methanol extract of Aplotaxis auriculata rhizome using GC\u2013MS","volume":"6","author":"Arunmathi C.","year":"2017","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4236\/jct.2014.58089"},{"key":"e_1_2_8_11_1","first-page":"53","article-title":"Isolongifolene attenuates oxidative stress and behavioral impairment in rotenone\u2010induced rat model of Parkinson's disease","volume":"8","author":"Balakrishnan R.","year":"2018","journal-title":"International Journal of Nutrition, Pharmacology, Neurological Diseases"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2021.08.017"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2309"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2014.931393"},{"key":"e_1_2_8_16_1","first-page":"1","article-title":"Chemical composition of the essential oil from Thapsia garganica L. 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