{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T12:45:41Z","timestamp":1789130741711,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Diabetes is marked by postprandial hyperglycemia (PHG), an abnormal rise in blood glucose after meals. A key therapeutic goal to reduce PHG is the inhibition of \u03b1\u2010amylase (\u03b1AM) and \u03b1\u2010glucosidase (\u03b1GL), enzymes that break down carbohydrates into sugars.\n                      <jats:italic>Cucurbita moschata<\/jats:italic>\n                      has been shown to inhibit both enzymes. However, its inhibition mechanism has not been explored. This study investigated the in vitro inhibition mechanisms of \u03b1AM and \u03b1GL and conducted a metabolomic analysis of\n                      <jats:italic>C. moschata<\/jats:italic>\n                      (edible part) water\u2010extract (CME), aiming to preliminarily identify its bioactive compounds (BCs). The inhibitory mechanisms were determined using Lineweaver\u2013Burk plots. The BCs were identified and quantified using HPLC\u2010QTOF\u2010MS, employing both targeted and untargeted metabolomic approaches. CME had a significant higher effect (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) on \u03b1AM activity than against \u03b1GL with IC\n                      <jats:sub>50<\/jats:sub>\n                      of 28.99 and 698.42\u00a0mg\/mL, respectively. The extract showed mixed and uncompetitive type inhibitions on \u03b1AM and \u03b1GL, respectively. The lowest inhibition constant (\n                      <jats:italic>\n                        K\n                        <jats:sub>i<\/jats:sub>\n                      <\/jats:italic>\n                      ) was 47.68\u00a0mg\/mL on \u03b1AM activity at 20\u00a0mg\/mL. Untargeted metabolic profiling by UPLC\u2010MS\u2010ESI\u2010QTOF putatively identified 30 compounds in CME, such as amino acids, vitamins, phytohormones, fatty acids, cucurbitacins and phenolic acids, and flavonoids. Functional analysis of CME identified significant pathways, including pantothenate and CoA biosynthesis and phenylpropanoids, among others. The targeted analysis by UPLC\u2010MS\u2010ESI\u2010QqQ allowed us to identify 12 compounds, with\n                      <jats:sc>l<\/jats:sc>\n                      \u2010phenylalanine,\n                      <jats:italic>p<\/jats:italic>\n                      \u2010hydroxybenzoic, and\n                      <jats:italic>p<\/jats:italic>\n                      \u2010coumaric acid as majors. This study demonstrated the inhibitory potential of CME on \u03b1AM and \u03b1GL activities, which may be attributed to its metabolites. Thus, this plant represents a valuable source of BC against PHG.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Practical Application<\/jats:bold>\n                      : The research highlights that\n                      <jats:italic>Cucurbita moschata<\/jats:italic>\n                      has significant potential in managing postprandial hyperglycemia in diabetic patients by inhibiting enzymes like \u03b1\u2010amylase and \u03b1\u2010glucosidase. In addition, the identification of its compounds emphasizes its importance as a source of bioactive compounds. Therefore,\n                      <jats:italic>C. moschata<\/jats:italic>\n                      could be effectively utilized in the development of nutraceuticals or as an ingredient in functional foods specifically designed for postprandial hyperglycemia management. Thus, integrating\n                      <jats:italic>C. moschata<\/jats:italic>\n                      as part of the daily diet could offer patients with diabetes a natural alternative to control their blood glucose levels after eating.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.17476","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T16:04:10Z","timestamp":1729613050000},"page":"9923-9935","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mechanistic in vitro study of the effect of\n                    <i>Cucurbita moschata<\/i>\n                    (Cucurbitaceae) on carbohydrate digestive enzymes"],"prefix":"10.1111","volume":"89","author":[{"given":"Jonatan Jafet","family":"Uuh\u2010Narvaez","sequence":"first","affiliation":[{"name":"Faculty of Chemical Engineering Autonomous University of Yucat\u00e1n  M\u00e9rida Yucat\u00e1n Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A.","family":"Guerrero\u2010Analco","sequence":"additional","affiliation":[{"name":"Laboratory of Chemistry of Natural Products, Network of Advanced Molecular Studies Institute of Ecology A. C.  Xalapa Veracruz Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan L.","family":"Monribot\u2010Villanueva","sequence":"additional","affiliation":[{"name":"Laboratory of Chemistry of Natural Products, Network of Advanced Molecular Studies Institute of Ecology A. C.  Xalapa Veracruz Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7664-6647","authenticated-orcid":false,"given":"Maira Rubi Segura","family":"Campos","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Engineering Autonomous University of Yucat\u00e1n  M\u00e9rida Yucat\u00e1n Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,22]]},"reference":[{"issue":"2","key":"e_1_2_8_2_1","first-page":"121","article-title":"In\u2010vitro antioxidant and \u03b1\u2010amylase inhibition activity of Cucurbita maxima","volume":"2","author":"Al\u2010Shaheen S. J. 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