{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T20:26:32Z","timestamp":1777407992314,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,28]],"date-time":"2024-12-28T00:00:00Z","timestamp":1735344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007104","name":"Trakya \u00dcniversitesi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007104","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      This study aimed to examine the extraction of specific phenolic compounds from blackthorn using ultrasound\u2010assisted extraction (UAE) and to evaluate the influence of UAE on the phenolic composition, bioaccessibility, and cytotoxic effect evaluated on ovarian cancer (OVCAR\u20103 and SKOV\u20103) and healthy (HaCaT) cell lines. The UAE parameters were optimized by modeling with the response surface method. Temperature, time, and ultrasound amplitude were utilized to determine the optimal extraction conditions. Optimization experiments were conducted to assess the influence of independent variables on the maximum concentrations of six phenolic compounds identified via high\u2010performance liquid chromatography (HPLC) and the 1,1\u2010diphenyl\u20102\u2010picrylhydrazyl (DPPH) radical scavenging activity. The optimal conditions for UAE were determined as an extraction temperature of 33.177\u00b0C, a duration of 4.334 min, and an amplitude of 38.980%. The phenolic profile of the extract obtained under optimized UAE conditions (B\u2010UAE\n                      <jats:sub>opt<\/jats:sub>\n                      ) was re\u2010examined, confirming that it remained consistent at the individual component level. Using an in vitro digestion model, UAE was found to increase the bioaccessibility of antioxidant activity in the small intestine. The IC\n                      <jats:sub>50<\/jats:sub>\n                      values for OVCAR\u20103 were 64 and 67\u00a0mg mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      for blackthorn and B\u2010UAE\n                      <jats:sub>opt<\/jats:sub>\n                      , while the IC\n                      <jats:sub>50<\/jats:sub>\n                      values for SKOV\u20103 were 53\u00a0mg mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      and 50\u00a0mg mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      for blackthorn and B\u2010UAE\n                      <jats:sub>opt<\/jats:sub>\n                      , respectively. This study showed that UAE is an exceptionally effective method for extracting phenolic compounds from blackthorn, as it preserves the phenolic profile, enhances bioaccessibility, and maintains cytotoxic effects without any detrimental impact.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.17614","type":"journal-article","created":{"date-parts":[[2024,12,28]],"date-time":"2024-12-28T12:19:47Z","timestamp":1735388387000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of ultrasound\u2010assisted extraction on phenolic profile, bio\u2010accessibility, OVCAR\u20103, and SKOV\u20103 cytotoxicity in blackthorn (\n                    <i>Prunus spinosa<\/i>\n                    L.)"],"prefix":"10.1111","volume":"90","author":[{"given":"Ayten","family":"Bostanci","sequence":"first","affiliation":[{"name":"Faculty of Engineering, Department of Genetics and Bioengineering Trakya University  Edirne Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1766-3762","authenticated-orcid":false,"given":"Emel","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Faculty of Engineering, Department of Food Engineering Trakya University  Edirne Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5521-2233","authenticated-orcid":false,"given":"Irem","family":"Damar","sequence":"additional","affiliation":[{"name":"Faculty of Engineering, Department of Food Engineering Trakya University  Edirne Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2009.10.016"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf072990e"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1556\/1326.2017.00327"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2015.06.014"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2010.11.023"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2006.12.011"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10030374"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2016.1274906"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11694\u2010022\u201001723\u20105"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.131001"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11130\u2010010\u20100159\u20103"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf073506a"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X0900401211"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.62313\/ijpbp.2024.161"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy10070966"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/horticulturae9091052"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.5222\/MMJ.2019.87864"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13399\u2010021\u201002150\u20100"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/horticulturae8111053"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.1878100"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24030573"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2022.104540"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27051691"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11203289"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijfs.13883"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2005.09.003"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750\u20103841.13398"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28083456"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217\u2010023\u201004263\u20104"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0113833"},{"key":"e_1_2_10_32_1","unstructured":"Popescu I. &Caudullo G.(2016).Prunus spinosa in Europe: Distribution habitat usage and threats. 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