{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T10:51:32Z","timestamp":1781952692957,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:sec>\n                    <jats:title>ABSTRACT<\/jats:title>\n                    <jats:p>\n                      Ethyl carbamate (EC) is a natural carcinogen widely found in fermented alcoholic beverages. The compound is mainly generated through the reaction of urea and citrulline with ethanol. The transcription factor Btn2p may affect arginine metabolism, and thus regulate EC in different fermentation systems. Therefore, in this study, we investigated the effects of Btn2p on arginine metabolism in\n                      <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                      in different culture systems and analyzed the potential regulatory mechanisms of EC formation. In addition, we studied the ethanol tolerance of\n                      <jats:italic>BTN2<\/jats:italic>\n                      \u2010modified yeast to determine its applicability in huangjiu fermentation and to provide a theoretical basis for subsequent studies. We found that\n                      <jats:italic>BTN2<\/jats:italic>\n                      knockout inhibited two major EC precursors, and the inhibitory effect was better in mixed cultures with\n                      <jats:italic>Pediococcus pentosaceus<\/jats:italic>\n                      . In addition,\n                      <jats:italic>BTN2<\/jats:italic>\n                      knockout promoted the activities of urease and ornithine transcarbamoylase, but reduced the activity of arginine deiminase, which led to the reduction of urea and citrulline concentrations. The growth conditions of\n                      <jats:italic>BTN2<\/jats:italic>\n                      \u2010modified strains under different ethanol concentrations were also studied for future applications in huangjiu fermentation. The results showed that\n                      <jats:italic>BTN2<\/jats:italic>\n                      overexpression promoted cell growth and increased ethanol tolerance, whereas\n                      <jats:italic>BTN2<\/jats:italic>\n                      knockout reduced the ethanol tolerance of cell. The findings indicated that Btn2p was involved in arginine metabolism, possibly through the regulation of urea and citrulline metabolism, and\n                      <jats:italic>BTN2\u2010<\/jats:italic>\n                      knockout strains can be used as a potential target for EC reduction.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70333","type":"journal-article","created":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T08:51:15Z","timestamp":1750150275000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Regulation of Arginine Metabolism by Transcription Factor Btn2p in a Mixed Culture of\n                    <i>Saccharomyces cerevisiae<\/i>\n                    and\n                    <i>Pediococcus pentosaceus<\/i>\n                    and Subsequent Ethanol Tolerance"],"prefix":"10.1111","volume":"90","author":[{"given":"Huqi","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Biological and Chemical Engineering Zhejiang University of Science and Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Xia","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering Zhejiang University of Science and Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tangchao","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering Zhejiang University of Science and Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anan","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering Zhejiang University of Science and Technology  Hangzhou 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