{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T10:09:29Z","timestamp":1780394969848,"version":"3.54.1"},"reference-count":66,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T00:00:00Z","timestamp":1757808000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Osteoporosis is characterized by a disrupted bone remodeling process, with bone resorption exceeding bone formation, resulting in reduced bone mass and impaired bone defect healing. Porphyran (PYP), a sulfated polysaccharide extracted from seaweed, inhibits osteoclast differentiation in vitro. This study aimed to explore the effects of PYP on osteoblasts and osteoclasts and on bone defect healing in ovariectomized (OVX) mice. In vitro, osteogenic and osteoclastic differentiation was assessed in PYP\u2010treated MC3T3\u2010E1 and BMMs using ALP staining, Alizarin Red staining, TRAP staining, immunofluorescence, qPCR, and western blotting. In vivo, 8\u2010week\u2010old female C57BL\/6 mice underwent ovariectomy followed by right maxillary incisor extraction to establish osteoporotic bone defects. The mice were divided into Sham, OVX, and PYP (OVX with PYP treatment) groups. The PYP group received daily oral gavage of PYP (200\u00a0mg\/kg), while the Sham and OVX groups received equivalent saline volumes. Extraction socket healing progression was assessed at 0, 7, and 21 days post\u2010extraction using ELISA, qRT\u2010PCR, \u00b5CT scanning, and histomorphometric analysis. The results showed that PYP did not enhance osteoblast differentiation but inhibited osteoclast differentiation by suppressing the NF\u2010\u03baB signaling pathway and promoted alveolar socket healing in OVX mice. In conclusion, porphyran promotes alveolar bone healing in osteoporotic mice by suppressing osteoclastogenesis via NF\u2010\u03baB signaling inhibition, suggesting its therapeutic potential for bone metabolic disorders.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Applications<\/jats:title>\n                    <jats:p>Porphyran (PYP), a natural seaweed compound, shows promise for creating bone\u2010health supplements or dental recovery treatments. It helps reduce bone loss after procedures such as tooth extractions in osteoporosis patients while protecting the body's natural bone\u2010building process. This plant\u2010based solution could lead to safer clinical therapies for doctors and accessible natural products for people wanting stronger bones. Its gentle action makes it suitable for both medical use and everyday consumer health products.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70447","type":"journal-article","created":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T11:49:51Z","timestamp":1757850591000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Porphyran Inhibits Osteoclast Formation Based on NF\u2010\u03baB Signaling Pathways and Accelerates Alveolar Bone Healing in Ovariectomized Mice"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0161-1906","authenticated-orcid":false,"given":"Pei","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Prosthodontics, Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Department of Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering Shanghai 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