{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T09:19:41Z","timestamp":1780046381843,"version":"3.53.1"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T00:00:00Z","timestamp":1754179200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Long\u2010term glucocorticoid therapy is known to induce osteoporosis by disrupting bone metabolism and altering gut microbiota. In this study, C57BL\/6J mice were used to establish a glucocorticoid\u2010induced osteoporosis (GIOP) model by intramuscular injection of dexamethasone. The mice were then divided into control, GIOP, and experimental groups; the experimental group was orally administered selenium\u2010enriched egg white protein (Se\u2010EWP) in order to evaluate its bone\u2010protective effects and potential mechanisms. The results showed that Se\u2010EWP significantly improved bone strength, trabecular microstructure, and bone mineral density in GIOP mice; enhanced the expression of bone synthesis\u2010related proteins, such as osterix and osteocalcin; and decreased the expression of bone catabolism\u2010related proteins, such as tartrate\u2010resistant acid phosphatase, receptor activator of nuclear factor\u2010\u03baB ligand, and cathepsin K. In addition, Se\u2010EWP was found to alleviate glucocorticoid\u2010induced gut microflora dysbiosis by increasing beneficial bacteria and microbial diversity. Se\u2010EWP significantly modulated the 5\u2010hydroxytryptamine receptor 1B (Htr1b) pathway, which is crucial for bone homeostasis and gut\u2013bone communication. The modulation of the Htr1b pathway suggests that Se\u2010EWP may exert its anti\u2010osteoporotic effects through this mechanism. These effects were statistically significant (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). Correlation analysis revealed significant associations between changes in the intestinal microbiota and bone metabolism parameters, further supporting the hypothesis that Se\u2010EWP exerts its effects through the gut\u2013bone axis. This suggests a novel therapeutic mechanism of action for Se\u2010EWP through the gut\u2013bone axis. In conclusion, this study provides strong evidence supporting the application of Se\u2010EWP in the prevention and treatment of GIOP.\n                  <\/jats:p>","DOI":"10.1111\/1750-3841.70450","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T03:49:33Z","timestamp":1754279373000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Selenium\u2010Enriched Egg White Protein Alleviates Glucocorticoid\u2010Induced Osteoporosis Mice via Gut Microbiota\u2010Driven Htr1b Inhibition"],"prefix":"10.1111","volume":"90","author":[{"given":"Da","family":"Wang","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering Northwest A&amp;F University  Xianyang Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Northwest A&amp;F 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