{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:01:45Z","timestamp":1772982105720,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      A high\u2010performance liquid chromatography with diode array detection (HPLC\u2010DAD) method was developed and validated to quantify puerarin encapsulated in exosome\u2010like nanoparticles derived from\n                      <jats:italic>Pueraria lobata<\/jats:italic>\n                      (ENsP). The method demonstrated high specificity, excellent linearity (\n                      <jats:italic>r<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      = 0.9999), and low limits of detection (LOD: 0.60 \u00b5g\/mL) and quantification (LOQ: 1.83 \u00b5g\/mL). Matrix effects were negligible, and accuracy and precision tests revealed high recovery rates (95.8%\u2013105.5%) with low relative standard deviations (0.58%\u20134.10%). Horwitz ratio values (0.15\u20130.66) and relative expanded uncertainties (13.8%\u201316.6%) complied with CODEX standards (\u226422%). The puerarin content in ENsP was determined to be 1.08 \u00b1 0.02\u00a0mg\/g, and its stability was maintained over 10 days at 4\u00b0C. In a cyclophosphamide (CPA)\u2010induced immunosuppressed BALB\/c mouse model, oral administration of ENsP significantly improved small intestinal morphology, including restoration of intestinal length and the villus\u2010to\u2010crypt ratio, and enhanced expression of barrier\u2010related genes and proteins ZO\u20101 and MUC2. These results highlight ENsP as a stable and biocompatible carrier with functional bioactivity, offering promising potential for pharmaceutical and nutraceutical applications targeting intestinal barrier integrity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Applications<\/jats:title>\n                    <jats:p>\n                      This study developed and validated an HPLC\u2010DAD method for the quantification of functional compounds in exosome\u2010like nanoparticles derived from\n                      <jats:italic>Pueraria lobata<\/jats:italic>\n                      (ENsP). This advancement establishes a foundation for quality control and the further development of functional food ingredients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70525","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T11:12:09Z","timestamp":1756897929000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Validated HPLC Analysis of Puerarin in\n                    <i>Pueraria lobata<\/i>\n                    \u2013Derived Exosome\u2010Like Nanoparticles With Intestinal Barrier Enhancement"],"prefix":"10.1111","volume":"90","author":[{"given":"Junho","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Food Science and Biotechnology Gachon University  Seongnam Gyeonggi\u2010do Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Hwan","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Gachon University  Seongnam Gyeonggi\u2010do Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miey","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Gachon University  Seongnam Gyeonggi\u2010do Republic of Korea"},{"name":"Institute for Aging and Clinical Nutrition Research Gachon University  Seongnam Gyeonggi\u2010do Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8353-3619","authenticated-orcid":false,"given":"Hae\u2010Jeung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Gachon University  Seongnam Gyeonggi\u2010do Republic of Korea"},{"name":"Institute for Aging and Clinical Nutrition Research Gachon University  Seongnam Gyeonggi\u2010do Republic of Korea"},{"name":"Department of Health Sciences and Technology, GAIHST Gachon University  Incheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2023.109511"},{"key":"e_1_2_9_3_1","unstructured":"AOAC (Association of Official Analytical Chemists).2016.Official methods of analysis of AOAC international. 20th ed. 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