{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T11:34:16Z","timestamp":1784806456144,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:00:00Z","timestamp":1789776000000},"content-version":"am","delay-in-days":383,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:00:00Z","timestamp":1758240000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011983","name":"American Radium Society","doi-asserted-by":"publisher","award":["58\u20103060\u20103\u2010022"],"award-info":[{"award-number":["58\u20103060\u20103\u2010022"]}],"id":[{"id":"10.13039\/100011983","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Sugar beet (\n                      <jats:italic>Beta vulgaris<\/jats:italic>\n                      L.) storage rots significantly reduce sugar quality and economic viability of the industry. Early storage rot detection can mitigate sugar loss through timely interventions, with volatile organic compounds (VOCs) serving as potential biochemical markers. However, conventional static headspace gas chromatography\u2010mass spectrometry (HS\u2010GC\u2010MS) methods for VOC detection typically require elevated incubation temperatures, unsuitable for accurately replicating real storage conditions (4\u00b0C\u201320\u00b0C), thus limiting their practical applicability. This study aimed to develop and optimize a low\u2010temperature static HS\u2010GC\u2010MS analytical method for accurate VOC profiling in stored sugar beets. A comparison between healthy and rotted sugar beet samples identified ethanol and ethyl acetate as two promising VOC markers for early detection of storage rots. Through systematic optimization of sample preparation, calibration strategies, and static headspace sampling parameters, a sensitive and reliable low\u2010temperature detection protocol was established. The optimized method effectively addressed matrix effects and enhanced analyte detection, yielding limits of detection (LOD) of 0.03 ppm for ethyl acetate and 1.4 ppm for ethanol, with recovery rates of 105% and 101%, respectively. This optimized approach provides a robust analytical foundation essential for integrating VOC profiling with sensor and machine learning technologies, significantly advancing real\u2010time sugar beet storage rot monitoring and management.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70546","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T09:58:51Z","timestamp":1758275931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Biomarkers of Rotted Sugar Beet: A Low\u2010Temperature Volatile Organic Compounds Analysis Framework Using Static Headspace Gas Chromatography\u2010Mass Spectrometry"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1978-5614","authenticated-orcid":false,"given":"Sujia","family":"Li","sequence":"first","affiliation":[{"name":"Department of Plant Sciences North Dakota State University  Fargo North Dakota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashish","family":"Christopher","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences North Dakota State University  Fargo North Dakota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences North Dakota State University  Fargo North Dakota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malick","family":"Bill","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS, Edward T. Schafer Agricultural Research Center Sugar Beet Research Unit  Fargo North Dakota USA"},{"name":"Department of Plant Pathology North Dakota State University  Fargo North Dakota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewumbua","family":"Monono","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Biosystems Engineering North Dakota State University  Fargo North Dakota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulaymon","family":"Eshkabilov","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Biosystems Engineering North Dakota State University  Fargo North Dakota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Braaten","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering North Dakota State University  Fargo North Dakota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyam L.","family":"Kandel","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS, Edward T. Schafer Agricultural Research Center Sugar Beet Research Unit  Fargo North Dakota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8276-2150","authenticated-orcid":false,"given":"Minwei","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences North Dakota State University  Fargo North Dakota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2019.125915"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2024.1342417"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","unstructured":"Bill M. S.Adhikari G. O.Bruni et\u00a0al.2025.\u201cFungal and Bacterial Species Associated With Storage Diseases in Sugar Beet From the Red River Valley of Minnesota and North Dakota.\u201dPlant Disease ahead of print August 13.","DOI":"10.1094\/PDIS-10-24-2188-RE"},{"key":"e_1_2_8_5_1","volume-title":"Storage","author":"Campbell L. G.","year":"2006"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp311504d"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.01.012"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12817"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1754-6834-6-158"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.21608\/ejp.2018.87799"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1071\/EN09013"},{"key":"e_1_2_8_12_1","first-page":"680","article-title":"Effects of Wilting, Drought and Temperature Upon Rotting of Sugar Beets During Storage","volume":"6","author":"Gaskill J. O.","year":"1950","journal-title":"Proceedings of the American Society of Sugar Beet Technologists"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00194-0"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00727-24"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471914584"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-019-0728-0"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiw004"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00226-1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(81)80134-3"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(01)00484-2"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2023.115715"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf000514h"},{"key":"e_1_2_8_23_1","unstructured":"Pihlstr\u00f6m T. 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