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Aflatoxin B\n                      <jats:sub>1<\/jats:sub>\n                      (AFB\n                      <jats:sub>1<\/jats:sub>\n                      ), a prevalent foodborne mycotoxin, causes oxidative stress, inflammation, and liver injury. However, the role of functional components of mulberry leaf in AFB\n                      <jats:sub>1<\/jats:sub>\n                      \u2010induced liver injury is still elusive. This study investigated the hepatoprotective potential of mulberry complex extract (MCE) and mulberry leaf polysaccharide (MLP) in a murine model of AFB1\u2010induced liver injury. Twenty\u2010four male mice were randomly divided into four groups, exposed to AFB\n                      <jats:sub>1<\/jats:sub>\n                      (0.75\u00a0mg\/kg), and treated with MCE (500\u00a0mg\/kg) or MLP (300\u00a0mg\/kg) for four weeks. Both treatments significantly improved liver function and reduced hepatic oxidative stress and inflammation. Additionally, they markedly inhibited NLRP3\/Caspase\u20101\/GSDMD\u2010mediated hepatocyte pyroptosis. These benefits were associated with activation of the Nrf2 pathway, upregulation of antioxidant genes and phase II detoxification enzymes, restoration of intestinal barrier integrity, and modulation of gut microbiota to enhance short\u2010chain fatty acid production. Notably, MCE and MLP interventions increased Nrf2 gene expression by 2.8\u2010 and 2.3\u2010fold, respectively. This study provides new evidence supporting the hepatoprotective effects of mulberry leaf extracts against AFB\n                      <jats:sub>1<\/jats:sub>\n                      \u2010induced toxicity. The findings highlight the potential of MCE and MLP as promising natural therapeutic agents for preventing or mitigating mycotoxin\u2010related liver damage through modulation of oxidative stress, inflammation, pyroptosis, and gut\u2010liver axis interactions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Applications<\/jats:title>\n                    <jats:p>Based on this research, mulberry leaf extracts could be developed into dietary supplements or functional food ingredients to help protect the liver in people regularly exposed to aflatoxin\u2010contaminated foods. Additionally, they show promise as natural additives in animal feed to reduce liver damage and improve the health of livestock consuming contaminated feedstuffs, potentially leading to safer animal products.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70600","type":"journal-article","created":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T19:34:07Z","timestamp":1759692847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Mulberry Leaf Extracts Ameliorate Aflatoxin B\n                    <sub>1<\/sub>\n                    \u2010Induced Liver Injury by Modulating Nrf2 Pathway and Gut Microbiota"],"prefix":"10.1111","volume":"90","author":[{"given":"Lixiao","family":"Shi","sequence":"first","affiliation":[{"name":"School of Life Sciences Anhui University  Hefei Anhui China"},{"name":"Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes Anhui University  Hefei Anhui China"},{"name":"Anhui Province Joint Construction Discipline Key Laboratory of Nanobody Technology  Hefei China"},{"name":"Anhui Healcurer Heath Biotech Co., Ltd \u2013 Anhui University Joint Postgraduate Training Base of Anhui Province  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejing","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui University  Hefei Anhui China"},{"name":"Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes Anhui University  Hefei Anhui China"},{"name":"Anhui Province Joint Construction Discipline Key Laboratory of Nanobody Technology  Hefei China"},{"name":"Anhui Healcurer Heath Biotech Co., Ltd \u2013 Anhui University Joint Postgraduate Training Base of Anhui Province  Hefei 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