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However, FC processing negatively affects fruit firmness, an important parameter for consumer acceptance. The objective of this work was to evaluate the effectiveness of the combined use of vacuum, calcium lactate, and pectin methylesterase (PME) to increase the shelf life and quality of FC strawberries. FC strawberry halves were subjected to vacuum infusion with water, PME (10UI), PME plus 1% calcium lactate, or 1% calcium lactate alone; FC strawberries without infusion were used as the control. Firmness, loss of fresh mass, anthocyanin, color, soluble solids content (SSC), titratable acidity (TA) SSC\/TA ratio, pH, visible spoilage and damage, visual quality, galacturonic acid content, degree of methylation, electrolyte efflux, PME activity, and respiration were measured initially and every 3 days for 9 days during 5\u00b0C storage. Vacuum infiltration of PME plus calcium lactate increased firmness by 90.7% for LCa\u00a0+\u00a0PME and 119.8% for LCa on Day 3, delayed weight loss to below 0.8% for all except PME treatments, maintained freshness and anthocyanin content, reduced electrolyte efflux by 38% compared to the control, and lowered respiration rates to 134.92 and 135.62\u00a0mg\u00a0kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      \u00a0h\n                      <jats:sup>\u22121<\/jats:sup>\n                      CO\n                      <jats:sub>2<\/jats:sub>\n                      for LCa\u00a0+\u00a0PME and LCa, respectively, whereas the control reached 163.36\u00a0mg\u00a0kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      \u00a0h\n                      <jats:sup>\u22121<\/jats:sup>\n                      CO\n                      <jats:sub>2<\/jats:sub>\n                      . Additionally, it minimized fruit damage. Therefore, vacuum infusion of 1% Lca\u00a0+\u00a0PME provided promising applications in extending the shelf life of cut strawberries by up to 3 days.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Applications<\/jats:title>\n                    <jats:p>This research offers a practical solution for the fresh\u2010cut fruit industry by improving the firmness and shelf life of strawberries through vacuum infusion with calcium lactate and PME. By maintaining texture, reducing weight loss, and slowing respiration, this method can help retailers provide fresher, higher\u2010quality strawberries with an extended shelf life of up to 3 days. Consumers would benefit from better\u2010tasting, longer\u2010lasting fresh\u2010cut fruit, reducing waste, and enhancing convenience.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70658","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T11:26:48Z","timestamp":1763378808000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Quality Maintenance and Increased Firmness of Fresh\u2010Cut Strawberries Using Vacuum Infusion of Calcium Lactate and Pectin Methylesterase"],"prefix":"10.1111","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0448-6998","authenticated-orcid":false,"given":"Alysson Caetano","family":"Soares","sequence":"first","affiliation":[{"name":"Fruit and Vegetables Laboratory, PPGAGRI, Department of Food Engineering Federal University of Sergipe (UFS)  S\u00e3o Crist\u00f3v\u00e3o Sergipe Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5647-4975","authenticated-orcid":false,"given":"M\u00f4nica Silva","family":"de Jesus","sequence":"additional","affiliation":[{"name":"Laboratory of Flavor and Chromatographic Analysis, Federal University of Sergipe  S\u00e3o Crist\u00f3v\u00e3o Sergipe Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5540-7831","authenticated-orcid":false,"given":"Steven A.","family":"Sargent","sequence":"additional","affiliation":[{"name":"Horticultural Sciences Department University of Florida\/IFAS  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0102-7128","authenticated-orcid":false,"given":"Jeffrey K.","family":"Brecht","sequence":"additional","affiliation":[{"name":"Horticultural Sciences Department University of Florida\/IFAS  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1589-0742","authenticated-orcid":false,"given":"Luiz Fernando Ganassali","family":"de Oliveira Junior","sequence":"additional","affiliation":[{"name":"Fruit and Vegetables Laboratory, PPGAGRI, Department of Food Engineering Federal University of Sergipe (UFS)  S\u00e3o Crist\u00f3v\u00e3o Sergipe Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2961-5557","authenticated-orcid":false,"given":"Marcelo Augusto Gutierrez","family":"Carnelossi","sequence":"additional","affiliation":[{"name":"Fruit and Vegetables Laboratory, PPGAGRI, Department of Food Engineering Federal University of Sergipe (UFS)  S\u00e3o Crist\u00f3v\u00e3o Sergipe Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.SCIENTA.2012.07.007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.POSTHARVBIO.2015.07.001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745\u20104514.1978.tb00193.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102621.2004.tb13354.x"},{"key":"e_1_2_9_6_1","volume-title":"Official Methods of Analysis of the Association of Official Analytical Chemists","author":"AOAC","year":"1997"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/IJE\/DYW319"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100\u201029452004000100009"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/FPLS.2018.01689"},{"key":"e_1_2_9_10_1","volume-title":"The Commercial Storage of Fruits, Vegetables, and Florist and Nursery Stocks","author":"Beaulieu J. 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